Xeno Islets • @1MEMO 20250113

Pig Islet Isolation, Fondation Transplantation, Strasbourg 1992 • Michel van der Burg • Miracles.Media • @1MEMO 20250113_1 • TakeNode b1f66781-cf63-4e64-a7b3-24cbdf5c107f

For the xenotransplantation research we started in 1991 at our Leiden Surgery Department, I studied pig islet isolation during a working visit February 1992, at the ‘Fondation Transplantation’ in Strasbourg-Hautepierre, France — here watching our student Vincent Nieuwenhuijs (left) at work in the international team – with Agnaldo Soares I guess – using the Ricordi method for islet isolation — and presented the recent Leiden islet work (1).

Notes

1) Van der Burg MPM, Gooszen HG. Islet preservation using UW, and islet function after
autotransplantation in dogs. Fondation Transplantation, CHU HautePierre Strasbourg
(France) February 18, 1992.

Update 20250124 – Paper reference

Citation info : Xeno Islets • Michel van der Burg • Miracles.Media • @1MEMO 20250113 • URL michelvanderburg.com/2025/01/13/

No Title • @1MEMO 20250112

No Title • Oil- Acrylic painting by Hans Dudart • Miracles.Media • @1MEMO 20250112_1 • TakeNode cbd20789-ebf5-4ede-925b-9b861c6719a4

Scan of print (10×15 cm; photo G.J. v.d. Poort) of oil- acrylic painting (60x50cm) by Hans Dudart.

Hans Dudart (1955-2003) worked as an artist in Holland, and as a valued co-worker in our Experimental Surgical Laboratory of the Leiden University Hospital (AZL, now LUMC) in Leiden, Holland. He died too soon from type 1 diabetes.

Citation info : No Title • Michel van der Burg • Miracles.Media • @1MEMO 20250112 • URL michelvanderburg.com/2025/01/12/

Pure Islets • @1MEMO 20250111

Pure Islets • Michel van der Burg • Miracles.Media • @1MEMO 20250111_1 • TakeNode a14f79f7-b04a-41ff-ab27-1de482a9fe4c

Slide film of May 11, 1989 developed by Audio Visual Center Leiden University.

Purified islets after using UW solution as the isolation medium, immuno-stained for insulin.

Citation info : Pure Islets • Michel van der Burg • Miracles.Media • @1MEMO 20250111 • URL michelvanderburg.com/2025/01/11/

Islet Transplants Performance 5th ESOT 1991 • @1MEMO 20250109

Islet Transplant Performance 5th ESOT 1991 • Michel van der Burg • Miracles.Media • @1MEMO 20250109_1 • TakeNode 75edbb4e-4360-43b8-8783-363ffd34c0ef

Poster (1) presented at the 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991. The poster was originally created on a Macintosh computer Oct 6, 1991, using the MacDraw II application, with graphs from Cricket Graph. That 1991 poster was remastered today in LibreOffice.

We presented 2 posters at the 5th ESOT, this poster (1), and another one on the effects of denervation with pancreas transplantation (2).

Two years after the 4th ESOT (in Barcelona, Nov 1989) where we introduced the UW-Solution as an islet isolation solution before density gradient purification, we now had a talk at this 5th ESOT on the use of the UW-Solution as the islet isolation solution, both before purification, and also during the purification in an innovative density gradient (3) — the foundation for current clinical purification methods. The talk was recorded on tape (except for the intro), and will be posted here soon too.

Notes

1) Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. Postprandial performance after canine islet transplantation: the importance of gut factors. 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991.

2) Guicherit OR, Van der Burg MPM, Lemkes HHPJ, Frölich M, Gooszen HG. Pancreatic
denervation causes reduced insulin sensitivity. 5 th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) Oct 7–10 1991.

3) Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991.

Citation info : Islet Transplant Performance 5th ESOT 1991 • Michel van der Burg • Miracles.Media • @1MEMO 20250109 • URL michelvanderburg.com/2025/01/09/