Tag: clinical

Human Islet Isolation • DRI Miami 1998 • @1MEMO 20250204

Summer 1997, Onno Terpstra, the head of our Surgery Department, asked me to explore our options for starting clinical islet isolation and transplantation in our center – the Leiden University Medical Center (LUMC), and prepare a roadmap.

September 1997, at the IPITA congress in Milan (1) , I learned from Camillo Ricordi, the scientific director of the Diabetes Research Institute (DRI) in Miami – where many guest collaborators work – that visiting the DRI some weeks might be a good option to gain sufficient first work experience in his clinical islet transplantation group.

December 1997, with a ‘Roadmap’, I got the green light to start the operationalization of clinical islet transplantation in the LUMC, first with a working visit to Miami to study the state of the art ‘Ricordi’ method of human islet isolation, and then start the human islet isolation work in our Surgery lab in Leiden.

In 1990 I had already gained some initial research experience in our Leiden Islet Laboratory with a small number of isolations of islets from human donor organs, using our innovative method of density gradient purification based on the UW solution (2).

During the first three months of 1998 I worked intensively with my student colleague Josephine Rijkelijkhuizen so that she would be able to continue our xenotransplantation research project as independently as possible in our Surgery research team.

April 1998, after participating in the 1st Workshop on Clinical Islet Transplantation in New Orleans (3) – the ideal introduction – a fruitful working visit to the DRI in Miami followed (4,5), with intensive collaboration with the DRI team on, among other things, six human islet isolations, and also the simultaneously (parallel) conducted research into the purification of the human islets in our Iodixanol gradient in UW-Solution (6).

Here a slideshow illustrating this team up with the DRI team in Miami , working on human islet isolation and purification in the innovative Iodixanol-UWS gradient, April-May 1998.

Notes

1) Innovative Islet Purification in Iodixanol-UWS • IPITA 97 • Michel van der Burg • Miracles.Media • @1MEMO 20250128 • TakeNode • URL michelvanderburg.com/2025/01/28/

2) Percoll UWS Purification Pancreatic Islets • ESOT 1991 Talk • Michel van der Burg • Miracles.Media • @1MEMO 20250114 • URL michelvanderburg.com/2025/01/14/

3) Invited participant 1st Workshop on Clinical Islet Transplantation, New Orleans (LA, USA) April 17–19, 1998

4) Working visit April – May 1998 at the “fast track” center of the Diabetes Research Institute (Scientific director Prof. C. Ricordi, MD) of the University of Miami (Miami, FL, USA)

5) DRI Dream Team • Michel van der Burg • Miracles.Media • @1MEMO 20250105 • URL michelvanderburg.com/2025/01/05/

6) Human Islet Purification Miami 1998 • Michel van der Burg • Miracles.Media • 20181102 • URL michelvanderburg.com/2018/11/02/

Citation info : Human Islet Isolation • DRI Miami 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250204 • TakeNode 86e4a319-a769-44d5-8fa5-de2ca90ab63c • URL michelvanderburg.com/2025/02/04/

Optiprep For Human Islet Purification • Cell Transplant Society 1999 • @1MEMO 20250203

Optiprep For Human Islet Purification • Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20250203 • TakeNode bad757b8-674c-4371-938c-ac73287bc457

Poster (#57) presentation Michel van der Burg, Cell Transplant Society congress 1999 in Montreux, Switzerland (1,2)

Outcome of the first series of human islet purifications with the novel Iodixanol -UWS density gradient — developed in the Leiden Islet Laboratory of the LUMC — performed during my working visit April – May 1998 at the “fast track” center of the Diabetes Research Institute (Scientific director Prof. C. Ricordi, MD) of the University of Miami (Miami, FL, USA).

Notes

1) Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Terpstra OT, Ricordi C. Optiprep for human islet purification. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999 .

2) Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Terpstra OT, Ricordi C. Optiprep for human islet purification (Abstract). Cell Transplant 1999; 8: 184. Download abstract below 1MEMO_20250202_3🔗
https://michelvanderburg.com/wp-content/uploads/2025/02/1memo_20250202_3.pdf

Citation info : Optiprep For Human Islet Purification • Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20250203 • URL michelvanderburg.com/2025/02/03/

Iodixanol-UWS Purification for Porcine Islet Transplantation • AIDSPIT 1998 • @1MEMO 20250131

Iodixanol-UWS Purification for Porcine Islet Transplantation • AIDSPIT 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250131

Slideshow (digitally remastered) of first talk on pancreatic islet xeno-transplantation following adult pig islet purification in our novel Iodixanol-UWS density gradient. Presented at the 17th Workshop of the AIDSPIT Study Group in Igls, Austria, Tuesday, Jan 27, 1998 (1, 2).

Isolation of porcine pancreatic islets (from the pancreas of large sows) was markedly improved by keeping the islets in the UW donor organ preservation solution (UWS) both during the isolation of islets (using the Liberase enzyme blend) and purification of the islets using a novel Iodixanol-UWS density gradient.
Transplantation of the pig islets (after 1 day storage in culture solution) restored normal blood sugar levels in diabetic mice.


20250131 1

Notes

1) Van der Burg MPM, Zwaan RP, Bouwman E. Markedly improved outcome of adult porcine islet isolation, purification, and culture using Liberase-PI versus collagenase-P, and a novel gradient of Optiprep in University of Wisconsin solution. 17th Workshop of the Study Group on Artificial Insulin Delivery Systems, Pancreas and Islet Transplantation (AIDSPIT) of the European Association for the Study of Diabetes (EASD), Igls (Austria) January 25—27,1998

2) Van der Burg MPM, Zwaan RP, Bouwman E. Markedly improved outcome of adult porcine islet isolation, purification, and culture using Liberase-PI versus collagenase-P, and a novel gradient of Optiprep in University of Wisconsin solution (Abstract). Horm Metab Res 1998; 30(1): A23 . DOI: 10.1055/s-2007-978834 .
Download abstract 1MEMO_20250131_2 below :

1MEMO_20250131_2🔗Download
Updates

Update 20260123 : Handout pdf (1MEMO_20250131_3) for download added.
Download handout presentation (1MEMO_20250131_3) below.

1MEMO_20250131_3Download

Citation info : Iodixanol-UWS Purification for Porcine Islet Transplantation • AIDSPIT 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250131 • TakeNode 1f185515-7aeb-44ac-b721-2cec76df67b7 • URL michelvanderburg.com/2025/01/31/

Innovative Islet Purification in Iodixanol-UWS • IPITA 97 • @1MEMO 20250128

No porcine islet loss during density gradient purification in a novel iodixanol in University of Wisconsin solution. First talk on our introduction of the innovative Iodixanol-UWS density gradients for islet purification , showing no islet loss in the difficult model of pig islet isolation — presented at the 6th IPITA congress in Milan (Italy) , Thursday, 25th september 1997 (1).

Background

In 1995 the Leiden Islet Lab had moved into the Surgery Lab in the new LUMC building, and the islet team had grown, focussing on xenotransplantation. With more people isolating pig islets, more convenient islet isolation solutions were tried.

Both Percoll-UWS (2) and Histopaque-UWS (3) were being used from 1992 in first series of islet isolations from juvenile pig pancreases.

August 1995, I started designing the clinical promising islet purification solution of Iodixanal in UW-Solution (UWS). I mixed Optiprep – a 60 % solution of iodixanol in water – with a 2-times concentrated UWS, to prepare this density gradient solution. The first standard operation procedure of this Iodixanol-UWS gradient for our pig islet isolation research was completed November 1995.

This purification solution was a logical follow-up of our highly succesful Percoll-UWS density gradient (2) — a similar solution of impermeants (that do not readily enter cells) with similar osmolarity (close to that of the tissue) , more practical though, and with the important advantage that iodixanol is clinical tested.

July 1996, I introduced the Iodixanol-UWS gradient to replace the Percoll-UWS gradient for large scale pancreatic islet research in this difficult model of islet isolation from juvenile pig pancreases.

The work was presented September 1997. First a poster was presented that month at the Xenotransplantation Congress in Nantes , France (4). Next, two talks followed at the 6th Congress of the International Pancreas and Islet Transplant Association (IPITA) in Milan (Italy).

Here the (remastered) slideshow of that first talk at the 6th IPITA Congress in Milan, presented Thursday, 25th september 1997 (1) . The slideshow of the 2nd talk , presented at the last day of that congress, was posted yesterday (5).

Notes

1) Van der Burg MPM, Basir I, Bouwman E. No porcine islet loss during density gradient purification in a novel iodixanol in University of Wisconsin solution. Sixth Congress of the Int. Pancreas and Islet Transplant Association (IPITA), Milan (Italy) Sept. 24–27, 1997.

2) Percoll UWS Purification Pancreatic Islets • ESOT 1991 Talk • Michel van der Burg • Miracles.Media • @1MEMO 20250114 • URL michelvanderburg.com/2025/01/14/

3) Basir I, van der Burg MPM, Scheringa M, Töns A, Bouwman E. Improved outcome of pig islet isolation by Pefabloc inhibition of trypsin. Transplant Proc. 1997 Jun;29(4):1939-41. doi: 10.1016/s0041-1345(97)00168-1. PMID: 9193462

4) Van der Burg MPM, Basir I, Bouwman E. Non-traumatic porcine islet density gradient purification in a novel iodixanol in University of Wisconsin solution. The 4th Int. Congress for Xenotransplantation, Nantes (France) Sept. 7–11, 1997. Online in : Purification Islets in Iodixanol-UWS • Leiden Start 1996 • Michel van der Burg • Miracles.Media • @1MEMO 20250126 • URL michelvanderburg.com/2025/01/26/

5) Van der Burg MPM, Basir I, Zwaan RP, Bouwman E. Porcine islet preservation during isolation in University of Wisconsin solution. Sixth Congress of the Int. Pancreas and Islet Transplant Association (IPITA), Milan (Italy) Sept. 24–27, 1997. Online in : Porcine Islet Preservation In UWS • IPITA 97 • Michel van der Burg • Miracles.Media • @1MEMO 20250127• URL michelvanderburg.com/2025/01/27/

Milan Papers • @1MEMO 20250205 • Manuscripts (Preprints) and Abstracts copies of the presentations at the IPITA 1997 meeting in Milan (Italy). PDF downloads at michelvanderburg•com/2025/02/05/

Citation info : Innovative Islet Purification in Iodixanol-UWS • IPITA 97 • Michel van der Burg • Miracles.Media • @1MEMO 20250128 • TakeNode • URL michelvanderburg.com/2025/01/28/

Porcine Islet Preservation In UWS • IPITA 97 • @1MEMO 20250127

August 1995, I started designing the clinical promising islet purification solution of Iodixanal in UW-Solution (UWS). I mixed Optiprep – a 60 % solution of iodixanol in water – with a 2-times concentrated UWS, to prepare this density gradient solution. The first standard operation procedure of this Iodixanol-UWS gradient for our pig islet isolation research was completed November 1995.

This purification solution was a logical follow-up of our highly succesful Percoll-UWS density gradient — a similar solution of impermeants (that do not readily enter cells) with similar osmolarity (close to that of the tissue) , more practical though, and with the important advantage that iodixanol is clinical tested.

The Leiden Islet Lab had just moved into the Surgery Lab in the new LUMC building, and the islet team had grown, focussing on xenotransplantation. With more people isolating pig islets, more convenient solutions were tried.

Both Percoll-UWS and Histopaque-UWS were being used from 1992 for small scale islet isolations from juvenile pig pancreases.

July 1996, I introduced the Optiprep-UWS gradient to replace our Percoll-UWS gradient for larger scale porcine islet isolations.

The work was first presented September 1997, both in a poster presented at the Xenotransplantation Congress in Nantes , France (1), and later that month in two talks at the 6th Congress of the International Pancreas and Islet Transplant Association (IPITA) in Milan (Italy). The slideshow of the first talk (2) will be online at this site soon.
Here the remastered slideshow of the 2nd talk at the 6th IPITA Congress in Milan, presented Saturday, 27th September, 1997 (3).

Notes

1) Van der Burg MPM, Basir I, Bouwman E. Non-traumatic porcine islet density gradient purification in a novel iodixanol in University of Wisconsin solution. The 4th Int. Congress for Xenotransplantation, Nantes (France) Sept. 7–11, 1997. Online in : Purification Islets in Iodixanol-UWS • Leiden Start 1996 • Michel van der Burg • Miracles.Media • @1MEMO 20250126 • URL michelvanderburg.com/2025/01/26/

2) Van der Burg MPM, Basir I, Bouwman E. No porcine islet loss during density gradient purification in a novel iodixanol in University of Wisconsin solution. Sixth Congress of the Int. Pancreas and Islet Transplant Association (IPITA), Milan (Italy) Sept. 24–27, 1997.

3) Van der Burg MPM, Basir I, Zwaan RP, Bouwman E. Porcine islet preservation during isolation in University of Wisconsin solution. Sixth Congress of the Int. Pancreas and Islet Transplant Association (IPITA), Milan (Italy) Sept. 24–27, 1997.

Milan Papers • @1MEMO 20250205 • Manuscripts (Preprints) and Abstracts copies of the presentations at the IPITA 1997 meeting in Milan (Italy). PDF downloads at michelvanderburg•com/2025/02/05/

Update 20250128 – update intro design timeline

Update 20250205 – added Milan papers

Citation info : Porcine Islet Preservation In UWS • IPITA 97 • Michel van der Burg • Miracles.Media • @1MEMO 20250127 • TakeNode 6d6fca2e-014c-40cf-8110-725b267c0b0e • URL michelvanderburg.com/2025/01/27/

Purification Islets in Iodixanol-UWS • Leiden Start 1996 • @1MEMO 20250126

Purification Islets in Iodixanol-UWS • Leiden Start 1996 • Michel van der Burg • Miracles.Media • @1MEMO 20250126_1 • TakeNode c2a26daf-2c4f-4051-931d-16babc872c82

August 1995, I started designing the clinical promising islet purification solution of Iodixanal in UW-Solution (UWS). I mixed Optiprep – a 60 % solution of iodixanol in water – with a 2-times concentrated UWS, to prepare this density gradient solution. The first standard operation procedure of this Iodixanol-UWS gradient for our pig islet isolation research was completed November 1995.


This purification solution was a logical follow-up of our highly succesful Percoll-UWS density gradient — a similar solution of impermeants (that do not readily enter cells) with similar osmolarity (close to that of the tissue) , more practical though, and with the important advantage that iodixanol is clinical tested.
The Leiden Islet Lab had just moved into the Surgery Lab of the new LUMC building, and the islet team had grown, focussing on xenotransplantation. With more people isolating pig islets, more convenient solutions were tried.

Both Percoll-UWS and and the more convenient Histopaque-UWS were being used from 1992 for small scale islet isolations from juvenile pig pancreases.

July 1996, I introduced the Optiprep-UWS gradient to replace our Percoll-UWS gradient for larger scale porcine islet isolations , in comparison with the Histopaque gradient.

This work was first presented September 1997, both in this poster presented at the Xenotransplantation Congress in Nantes (France) and later that month also in a talk at the 6th Congress of the International Pancreas and Islet Transplant Association (IPITA) in Milan (Italy) — presentations that will be online at this site soon.

Notes

Van der Burg MPM, Basir I, Bouwman E. Non-traumatic porcine islet density gradient purification in a novel iodixanol in University of Wisconsin solution. The 4th Int. Congress for Xenotransplantation, Nantes (France) Sept. 7–11, 1997.

Update 20250128 – update intro design timeline

Citation info : Purification Islets in Iodixanol-UWS • Leiden Start 1996 • Michel van der Burg • Miracles.Media • @1MEMO 20250126 • URL michelvanderburg.com/2025/01/26/

DRI Dream Team • @1MEMO 20250105

DRI Dream Team • Miami 1998 • Michel van der Burg • Miracles.Media • 20250105_1 • TakeNode e6258505-55a8-4365-bb70-7a1dcd7b5426

Team up with researchers at the Diabetes Research Institute in Miami , working on human islet transplantation (1).

“For National Geographic” announced Camillo Ricordi for fun, that Monday , April 27th in 1998 at the Diabetes Research Institute, when the DRI islet isolation team posed in GMP outfit for this picture (2) — with Elina Linetsky on the left , and me standing second from right — taken after finishing our human islet isolation work, including islet purification in the iodixanol gradient in UW-Solution (3).

Notes

1) Working visit April – May 1998 at the “fast track” center of the Diabetes Research Institute (Scientific director Prof. C. Ricordi, MD) of the University of Miami (Miami, FL, USA)

2) Blown up image (from small GIF online in the early DRInet days), taken through a reflecting window.

3) Human Islet Purification Miami 1998 • Michel van der Burg • Miracles.Media • 20181102 • URL michelvanderburg.com/2018/11/02/

Citation info : DRI Dream Team • Michel van der Burg • Miracles.Media • @1MEMO 20250105 • URL michelvanderburg.com/2025/01/05/