Screenshot April 11, 1995 at 22:04 of the desktop with file search window on my Apple Macintosh PowerBook 160. Desktop shows icon (renamed ‘Net’) of web browser NCSA Mosaic. A PICT file image that I recently gained access to.
#Apple #Powerbook #Mosaic #internet #history #desktop #intransition
Citation info : Desktop 1995 Macintosh PowerBook 160 • Michel van der Burg • Miracles•Media • @1MEMO 20250514 • TakeNode e29d72db-e6a0-4e6c-9474-9822ec7f854f • • URL 1-memo.com/2025/05/14 •
Jun 21, 2007, I started pioneering at YouTube, creating Channel012.
The first YouTube video ‘Fire Breather’ was shot at a 4:3 aspect ratio (640×480 pixels) and produced at a 16:9 aspect ratio with black bars for my first YouTube upload on Dec 30, 2007.
Next a 2nd edition of ‘Fire Breather’ was produced at the original 4:3 aspect ratio, and uploaded the next month, Jan 18, 2008.
YouTube displayed that 2nd edition at a higher quality. Sometime later though I decided to change visibility of that 2nd edition to ‘unlisted’ in order to preserve and favor the 1st edition views count.
More recently that forgotten unlisted 2nd edition video appeared to be lost somehow…until I learned today that YouTube had changed visibility in 2021 of all unlisted videos uploaded before 2017 to ‘private’ automatically for security reasons with a worldwide system change.
Today I changed visibility at YouTube of that 2nd edition (video shown above) to public.
Still ‘Fire Breather • 2nd Edition’ • @1MEMO 20250509 • TakeNode bc90fb98-6a51-4c98-9ad6-1502d11fb7d2
Channel012 :
YouTube – Channel012 – First upload , Dec 30, 2007 : Fire Breather • @1MEMO 20071230 • youtube.com/channel012 • URL https://youtu.be/cJDIJg0qhl8
Fire Breather | 2nd Edition • @1MEMO 20080118 • TakeNode 541d345c-ff23-495f-b0c2-8564ce90af17
YouTube team. Update to YouTube Unlisted Links. Jun 23, 2021. URL https://blog.youtube/news-and-events/update-youtube-unlisted-links/
Inspiration :
Rosas-Salazar Vladimir (2025), “Fading Echoes: Remembering and Forgetting through Videotapes.” [in]Transition 12(1). DOI: https://doi.org/10.16995/intransition.16950
Tags :
#lost #film #edition #unlisted #YouTube #memory #fragment #digital #intransition #fire #firebreather #performance #Holland #art #pioneer #channel012
Citation info : Lost in Transition • Michel van der Burg • Miracles.Media • @1MEMO 20250509 • URL 1-memo.com/2025/05/09 •
Fire breather Ronald Klok in the ‘Vischhandel’ bar in Haarlem, Holland (Dec 8, 2007). Vertical edition of my first YouTube video – published 17 years ago, Dec 30, 2007.
Citation info : Fire Breather • Michel van der Burg • Miracles.Media • @1MEMO 20250507 • TakeNode c44eb912-70c2-4f07-a0c2-4b20a3ecbb50
Summer 1997, Onno Terpstra, the head of our Surgery Department, asked me to explore our options for starting clinical islet isolation and transplantation in our center – the Leiden University Medical Center (LUMC), and prepare a roadmap.
September 1997, at the IPITA congress in Milan (1) , I learned from Camillo Ricordi, the scientific director of the Diabetes Research Institute (DRI) in Miami – where many guest collaborators work – that visiting the DRI some weeks might be a good option to gain sufficient first work experience in his clinical islet transplantation group.
December 1997, with a ‘Roadmap’, I got the green light to start the operationalization of clinical islet transplantation in the LUMC, first with a working visit to Miami to study the state of the art ‘Ricordi’ method of human islet isolation, and then start the human islet isolation work in our Surgery lab in Leiden.
In 1990 I had already gained some initial research experience in our Leiden Islet Laboratory with a small number of isolations of islets from human donor organs, using our innovative method of density gradient purification based on the UW solution (2).
During the first three months of 1998 I worked intensively with my student colleague Josephine Rijkelijkhuizen so that she would be able to continue our xenotransplantation research project as independently as possible in our Surgery research team.
April 1998, after participating in the 1st Workshop on Clinical Islet Transplantation in New Orleans (3) – the ideal introduction – a fruitful working visit to the DRI in Miami followed (4,5), with intensive collaboration with the DRI team on, among other things, six human islet isolations, and also the simultaneously (parallel) conducted research into the purification of the human islets in our Iodixanol gradient in UW-Solution (6).
Here a slideshow illustrating this team up with the DRI team in Miami , working on human islet isolation and purification in the innovative Iodixanol-UWS gradient, April-May 1998.
Notes
1) Innovative Islet Purification in Iodixanol-UWS • IPITA 97 • Michel van der Burg • Miracles.Media • @1MEMO 20250128 • TakeNode • URL michelvanderburg.com/2025/01/28/
2) Percoll UWS Purification Pancreatic Islets • ESOT 1991 Talk • Michel van der Burg • Miracles.Media • @1MEMO 20250114 • URL michelvanderburg.com/2025/01/14/
3) Invited participant 1st Workshop on Clinical Islet Transplantation, New Orleans (LA, USA) April 17–19, 1998
4) Working visit April – May 1998 at the “fast track” center of the Diabetes Research Institute (Scientific director Prof. C. Ricordi, MD) of the University of Miami (Miami, FL, USA)
5) DRI Dream Team • Michel van der Burg • Miracles.Media • @1MEMO 20250105 • URL michelvanderburg.com/2025/01/05/
6) Human Islet Purification Miami 1998 • Michel van der Burg • Miracles.Media • 20181102 • URL michelvanderburg.com/2018/11/02/
Citation info : Human Islet Isolation • DRI Miami 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250204 • TakeNode 86e4a319-a769-44d5-8fa5-de2ca90ab63c • URL michelvanderburg.com/2025/02/04/
Poster (#57) presentation Michel van der Burg, Cell Transplant Society congress 1999 in Montreux, Switzerland (1,2)
Outcome of the first series of human islet purifications with the novel Iodixanol -UWS density gradient — developed in the Leiden Islet Laboratory of the LUMC — performed during my working visit April – May 1998 at the “fast track” center of the Diabetes Research Institute (Scientific director Prof. C. Ricordi, MD) of the University of Miami (Miami, FL, USA).
1) Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Terpstra OT, Ricordi C. Optiprep for human islet purification. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999 .
2) Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Terpstra OT, Ricordi C. Optiprep for human islet purification (Abstract). Cell Transplant 1999; 8: 184. Download abstract below 1MEMO_20250202_3🔗
https://michelvanderburg.com/wp-content/uploads/2025/02/1memo_20250202_3.pdf
Citation info : Optiprep For Human Islet Purification • Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20250203 • URL michelvanderburg.com/2025/02/03/
Slideshow (digitally remastered) oral presentation Michel van der Burg, Cell Transplant Society congress 1999 in Montreux, Switzerland (1)
The Leiden Islet Laboratory developed a novel Iodixanol-UWS density gradient for purification of the islets of Langerhans from the pancreas in the difficult pig model.
Pig islet isolation from the pancreas of slaughterhouse sows was markedly improved by keeping the islets in the UW donor organ preservation solution (UWS) both during the isolation of islets (using the Liberase enzyme blend) and purification of the islets using our novel Iodixanol-UWS density gradient.
Xeno transplantation of these 1-day-cultured pig islets restored normal blood sugar levels in diabetic mice.
Iodixanol-UWS Purification and Culture of Pig Islets Xeno Transplants • Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20250202_2 • TakeNode 8bd79256-78b2-4d8e-8d48-6da41102dc2d • Download file available below 1MEMO_20250202_2
1) Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Adult pig islet recovery during Liberase isolation, OptiPrep purification and culture for transplantation in nude mice. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999 .
2) Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Adult pig islet recovery during Liberase isolation, OptiPrep purification and culture for transplantation in nude mice (Abstract). Cell Transplant 1999; 8: 185. Download abstract, available below 1MEMO_20250202_3
Citation info : Iodixanol-UWS Purification and Culture of Pig Islets Xeno Transplants • Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20250202 • TakeNode f9d1fdc5-21e4-4932-b995-4c97639945fe • URL michelvanderburg.com/2025/02/02/
Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Culture of isolated pig islets pre-xenotransplantation greatly improves the quality and survival of the graft (Kweek van geïsoleerde varkenseilandjes pre-xenotransplantatie verbetert aanzienlijk de kwaliteit en overleving van het transplantaat). Tenth Congress of the Dutch Transplantation Society (Bootcongres 1998), Kerkrade (The Netherlands) April 21–23, 1998.
Download (pdf) NTV bulletin (abstract) & poster , file 1MEMO_20250201
PRE-TRANSPLANT SHORT-TERM CULTURE OF ISOLATED PIG ISLETS MARKEDLY IMPROVES THE QUALITY AND SURVIVAL OF THE XENOGRAFT
Van der Burg MPM,* Rijkelijkhuizen JKRA, Zwaan RP, and Bouwman E, Leiden University Medical Centre, Leiden, The Netherlands.
Pre-transplant culture of isolated islets reduces the graft’s immunogenicity in various models. Little is known, however, on the effect of culture in pig islet xenotransplantation — probably, because porcine islets have been found difficult to culture. Because pilot transplants of freshly-isolated pig islets in strongly immunosuppressed rats resulted in primary non function, we first studied 1-7 day culture of the isolated pig islets at 37°C in RPMI plus 10% porcine serum. Islets were isolated from large sows (n =6) by an improved method, resulting in intact islets (size 185±16 µm) and no loss during purification, yielding 2448 islets (IEQs)/g with a >95% purity and 90±2% viability as assessed by acridine orange – propidium iodide (AOPI) staining. During culture, however, islet recovery was 24±9% at day 1 and 17±6% at day 7 (NS vs day 1). In order to delineate whether the culture conditions or the quality of freshly isolated islets caused the islet loss during culture, we compared graft survival in nude mice at 1 mo after transplantation under the kidney capsule of ~2500 fresh islets in normoglycemic recipients or ~1000 cultured islets in STZ-diabetic (>20 mM) recipients. After transplanting fresh islets, histology of the kidneys — sectioned every 500 µm — demonstrated substantial scarring and (near-)absence of islets. Cultured islet transplants, by contrast, rendered 5/6 recipients normoglycemic, and showed a substantial mass of well-preserved islets with little scarring at the grafts’ site. Thus, viability by AOPI-staining of fresh islets poorly predicts the survival in vivo and in vitro. A non-immune mediated disintegration of part of the fresh islets may substantially reduce the functional capacity of the graft, both direct by lowering the effective islet dose, and indirect first because scarring may hamper the engraftment of viable tissue, and second because the cellular debris most probably will attract macrophages and induce the release of harmful cytokines.
Citation info : Fresh vs Cultured Pig Islets for Xenografts • NTV Bootcongres 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250201 • URL michelvanderburg.com/2025/02/01/
