Leiden Islet Laboratory History • 20240803

Leiden Islet Laboratory History • 20240803 | Michel van der Burg | michelvanderburg•com | TakeNode fad1be86-34f2-453f-b477-2abc62e467be

Summer 1985… start of our Leiden Islet Transplant Laboratory by Paul van Suylichem (right) and Michel van der Burg (middle), both from the Surgery department — wondering how to start here our islet lab in this space – a former weighing chamber (left : stable weighing table) made available by the Cell Biology department of the Leiden University, headed by Prof J.P. Scherft. Where to put our equipment… the laminar flow cabinet, large centrifuge, fridge, freezer, shaking waterbath, microscopes, and a lot of disposables coming ?

I had started working in 1981 for the Pancreas project. Pancreas transplantation research with the surgeon Hein Gooszen , working on his PhD (1), and surgeon Reinout van Schilfgaarde – who headed the project – and in 1985 also started this islet project.

Summer 1986, when Reinout van Schilfgaarde together with Paul van Suylichem moved to the Groningen University Hospital (2), Hein Gooszen and I decided to make a fresh start with the now operational Leiden Islet Lab…


Fifteen years of research later we were ready to roll with the isolation of human islets of Langerhans, in our new GMP facility in the Leiden Univerity Medical Center (LUMC). September 2001, the National Health Care Institute positively assessed our permit application for Transplantation of Islets of Langerhans in the LUMC (3,6), followed by approval May 2003 of the islet bank by the Health and Youth Care Inspectorate of the Ministry of Health, Welfare and Sport (VWS).

Notes

Post last update : 20241010-1605

  1. Gooszen HG. Canine segmental pancreatic autotransplantation. Analysis of the effects of ductobliteration. Thesis, Leiden, 1984
  2. Benoemd – Te Groningen dr.R.van Schilfgaarde tot hoogleraar in de heelkunde. Ned Tijdschr Geneeskd. 1986;130:2240 | URL https://www.ntvg.nl/artikelen/personalia-7848
  3. Advies vergunningsaanvraag transplantatie Eilandjes van Langerhans | Op 27 september 2001 uitgebracht aan de minister van Volksgezondheid, Welzijn en Sport. | Rapport 01/71 | C.R. Smand, J.P. Verduijn / College voor Zorgverzekeringen. URL https://data.bibliotheken.nl/id/nbt/p229940978
  4. Update 20240807. Added some history, and made minor changes.
  5. Update 20240828 minor correction date (Fresh start lab ‘At the end of 1986’ changed to Summer 1986.)
  6. Update 20241010, added : Press release Sep 27, 2001 by National Health Care Institute (CVZ). Transplantatie Eilandjes van Langerhans naar LUMC 27 september 2001. CVZ adviseert over vergunning. John van der Pas, persvoorlichter CVZ. Source : Razende Robot Reporter | Nieuwsbank Interactief Nederlands persbureau .
    Retrieved 20090313 from Nieuwsbank.nl (link expired, domain has new owner).
    20241010 PDF (Ads , and author phone number redacted by M.P.M. van der Burg | Miracles•Media) file 20241010_CVZ • URL michelvanderburg.com/2024/10/10

Citation info : Leiden Islet Laboratory History • 20240803 | Michel van der Burg | michelvanderburg•com | TakeNode fad1be86-34f2-453f-b477-2abc62e467be

Pancreatic islet transplantation Thesis Repository Leiden University | 20221125

Pancreatic islet transplantation Thesis Repository Leiden University | 20221125 | Michel van der Burg | Miracles•Media

Pancreatic islet transplantation , the doctoral thesis by Michel van der Burg – digital publication ISBN 9789080216402 – is freely available (Open Access) now in the Scholarly Publications repository of Leiden University. Persistent URL of this record https://hdl.handle.net/1887/3486604

The dissertation ‘Pancreatic islet transplantation’ was published first as a paperback in 1994 ( ISBN paperback 9789080216419 ) — and obviously that is considered virtually inaccessible in this digital age. Though it is still available ‘new’ — collectors item ;)
Last October the digital edition ISBN eBook (pdf) 9789080216402 has been produced and first released by the author / publisher Michel van der Burg at Miracles•Media as a free download , available from October 25th, 2022.

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Stellingen | Addendum e-Book ISBN_9789080216402 | 20221026

Stellingen | Addendum e-Book ISBN_9789080216402 | 20221026 | Michel van der Burg | Miracles•Media

Free download available now of the Addendum e-book (pdf) ‘Propositions | Stellingen (dutch)’ belonging to the dissertation ‘Pancreatic islet transplantation’ by Michel P.M. van der Burg (addendum eBook ISBN 9789080216402) .

Preview & Download available at Miracles.Media page https://miracles.media/pancreatic-islet-transplantation-ebook-isbn-9789080216402/

Note : The Dutch dissertation system has a tradition of accompanying the generally very specialised PhD thesis with ten or more propositions (other ‘theses’), that should be ‘defensible’, and while at least some should be related to the dissertation, others generally reflect the PhD candidate’s broader interests, and the last one is often playful.

TAGS #addendum #stellingen #propositions #ebook #transplantation #islets #technology #thesis #medicine #ISBN9789080216402 #book #release #distibution #cover #FrenchFlap #flap #publish #blurb #science #donor #pancreas #insulin #therapy #clinical #isletsofLangerhans #isolation #University #Wisconsin #solution #UWS #density #gradient #separation #biology #diabetes #IDDM #laboratory #research #cure #Leiden #University #LUMC #dissertation #PhD #DoctorofPhilosophy #publisher #scan #ISBN9789080216419 #Holland #Netherlands #TakeNode #MiraclesMedia #1Memo #michelvanderburg

Pancreatic islet transplantation | eBook ISBN 9789080216402

Pancreatic islet transplantation | eBook ISBN 9789080216402 | 20221025 | Michel van der Burg | Miracles•Media

Pancreatic islet transplantation : studies on the technique and efficacy of islet isolation and transplantation. PhD Thesis (English) by Michel P.M. van der Burg.

The doctoral thesis ‘Pancreatic islet transplantation’ was published first as a paperback in 1994 — and obviously that is considered virtually inaccessible in this digital age. Now this e-Book edition has been produced and released by the author / publisher Michel van der Burg | Miracles•Media as a free download , available from October 25th, 2022.

Download

Download eBook ‘Pancreatic islet transplantation’ by Michel van der Burg | ISBN eBook (pdf) 978-90-802164-0-2 for free at the author’s / publishers’ site Miracles•Media here :
https://miracles.media/pancreatic-islet-transplantation-ebook-isbn-9789080216402/

Support – For people who would like to also support my work – you can … ‘Buy me a coffee’ there too ;)

eBook details

Title : Pancreatic islet transplantation : Studies on the technique and efficacy of islet isolation and transplantation

Author : Michel P.M. van der Burg

Publisher : M.P.M. van der Burg | Miracles.Media

Genre : Medicine Technology & Science & Nature

Released : October 25, 2022

Language : EN English & Dutch summary

Length : 192 pages / 68 MB

Format : PDF (full access, no DRM, searchable PDF – designed for long term accessibility and archive PDF/A-1b – PDF/UA)

Version : Reprinted 1st edition (2nd impression) as eBook (pdf ISBN 9789080216402) by author / publisher M.P.M. van der Burg from scanned paperback published in 1994 by M.P.M. van der Burg (ISBN paperback 9789080216419).

Requirements : You can view this book on any device, best with a tablet or larger screen.

Addendum : Propositions | Stellingen (dutch) – published – release here will follow later.

Review / Award : Glaxo Wellcome Gastrointestinal Research Award – 2nd Prize – awarded October 5, 1995 to Michel P.M. van der Burg for the dissertation “Pancreatic islet transplantation” (received at the Fall meeting of the Dutch Gastroenterology Society, Veldhoven, The Netherlands)

File name : 9789080216402_ISBN.pdf

TakeNode ID : b9a348fd-9f3e-4f02-af51-e1ab25d61522

Publisher’s description

Pancreatic islet transplantation by a new method is introduced here : the isolation of islets in an organ preservation solution, the University of Wisconsin solution. A new concept allowing for the first time large-scale isolation and transplantation of consistently near 100% pure islets of Langerhans in a preclinical dog model with fasting normoglycemia up to 3 years posttransplant. Detailed metabolic studies demonstrated normal insulin levels after meals with preservation of gut hormone action stimulating insulin secretion at the mild hyperglycemia after meals. The introduction of the University of Wisconsin solution organ preservation solution for islet isolation at the start of these studies in 1989 is a new concept … and has been shown by now in 2022 , world-wide , to make the future of islet isolation and transplantation methods for insulin-dependent diabetes mellitus (IDDM).

TAGS #ebook #transplantation #islets #technology #thesis #medicine #ISBN9789080216402 #book #release #distibution #cover #FrenchFlap #flap #publish #blurb #science #donor #pancreas #insulin #therapy #clinical #isletsofLangerhans #isolation #University #Wisconsin #solution #UWS #density #gradient #separation #biology #diabetes #IDDM #laboratory #research #cure #Leiden #University #LUMC #dissertation #PhD #DoctorofPhilosophy #publisher #scan #ISBN9789080216419 #Holland #Netherlands #TakeNode #MiraclesMedia #1Memo #michelvanderburg

Cover | Pancreatic islet transplantation | ISBN 9789080216402 | 20221024

Cover | Pancreatic islet transplantation | ISBN 9789080216402 | 20221024 | Michel van der Burg | Miracles•Media | PUBLISHED ! Here the full cover with a French flap back-cover of the e-Book (pdf) edition of my doctoral thesis ‘Pancreatic islet transplantation’ – ISBN 9789080216402 – published and registered today, Oct 24th, 2022 . In short : Pancreatic islet isolation in the University of Wisconsin solution is a new concept introduced here … allowing transplantation of pure islets as an effective treatment in insulin-dependent diabetes. Distribution of the e-Book and full information follows this week.

TakeNode ID: c50c9112-5763-4117-b31a-e818723e18a2

TAGS #cover #FrenchFlap #flap #publish #blurb #science #donor #pancreas #insulin #therapy #clinical #isletsofLangerhans #isolation #UniversityofWisconsin #solution #UWS #density #gradient #separation #transplantation #medicine #biology #diabetes #IDDM #laboratory #research #cure #LeidenUniversity #LUMC #dissertation #PhD #DoctorofPhilosophy #publisher #scan #ebook #book #ISBN9789080216402 #ISBN9789080216419 #Holland #Netherlands #TakeNode #MiraclesMedia #1Memo #michelvanderburg

Blurb | Pancreatic islet transplantation | 20221023

Blurb | Pancreatic islet transplantation | 20221023 | Michel van der Burg | Miracles•Media | French flap interior of paperback back cover with illustration of islet isolation and transplantation in the doctoral thesis Pancreatic islet transplantation – ISBN 9789080216402. Studies aiming at transplantation of the pancreatic Islets of Langerhans to prevent, postpone or ameliorate the long-term crippling complications of insulin-dependent diabetes mellitus (IDDM). Paperback published in 1994 ISBN 9789080216419 | 9080216410 , later this month published as eBook (pdf) ISBN 9789080216402.

Illustration of Islet Isolation and Transplantation
(Captions at last page of the dissertation)

1. Paul Langerhans
2. Section of the normal dog pancreas showing islets stained reddish-brown (immunostained for insulin)
3. The author, watching the dog islet isolation procedure in the Minneapolis surgical research laboratory
4. Start of the operation in the dog (From left to right: Professor Hein G. Gooszen, Ms. M. Jane Field (Minneapolis), and Dr. Onno R. Guicherit, at our surgical laboratory)
5. The pancreas is removed
6. Infusion of the collagenase solution via the ducts in the dog pancreas – collagenase leaking from the pancreas is recirculated using a pump (Islet laboratory, at the Department of Cell Biology and Histology, Leiden)
7. During collagenase digestion at 37-39°C the pancreas falls apart (non-stained pancreatic exocrine tissue and free-ed small blood vessel, at low magnification)
8. Dissociated pancreatic tissue
9. Tissue is further dispersed by aspiration in a syringe, and sieved to remove undigested fragments, ducts and vessels ~ demonstrated by Ms. Jane Field at our islet laboratory.
10. Tissue suspension demonstrating low purity of the islets (stained red by dithizone) obtained by dextran gradient separation after isolation in the RPMI tissue culture solution (dark-brown exocrine fragments remain unstained)
11. Pure islet suspension (after dithizone staining) obtained by density separation in Percoll gradients (in University of Wisconsin solution) after islet isolation in the University of Wisconsin solution (islets are only slightly stained due to poor diffusion over the cell membrane in the preservation solution)
12. Section of highly purified islets obtained by dextran density separation after isolation in the University of Wisconsin solution
13. Islets are autotransplanted by infusion in the spleen of the dog
14. Transplanted islet in section of the spleen of one of the dogs shortly after the onset of fasting hyperglycemia at three months posttransplant (immunostained for insulin)
15. Highly purified human islets obtained by Percoll density separation after isolation in the University of Wisconsin solution (immunostained with gold for insulin; not counterstained)

TakeNode ID: 62602d86-7244-4d1e-8d10-560cf963ee76

TAGS #blurb #FrenchFlap #flap #cover #publish #science #donor #pancreas #insulin #therapy #clinical #isletsofLangerhans #isolation #UniversityofWisconsin #solution #UWS #density #gradient #separation #transplantation #medicine #biology #diabetes #IDDM #laboratory #research #cure #LeidenUniversity #LUMC #dissertation #PhD #DoctorofPhilosophy #publisher #scan #ebook #book #ISBN9789080216402 #ISBN9789080216419 #Holland #Netherlands #TakeNode #MiraclesMedia #1Memo #michelvanderburg

Reprint | 20221022


Reprint | 20221022 | Michel van der Burg | 1-memo•com | Silent film. Scanning for an ebook reprint – ISBN 9789080216402 – of the doctoral thesis Pancreatic islet transplantation. Studies on the isolation and transplantation of the pancreatic Islets of Langerhans to prevent, postpone or ameliorate the long-term crippling complications of insulin-dependent diabetes mellitus (IDDM). First published as a paperback in 1994 ISBN 9080216410 / 9789080216419 , and later this month published as eBook (pdf). TakeNode ID: 777e40a7-c148-4832-a24a-ff599c1845c5

TAGS #publish #science #donor #pancreas #insulin #therapy #clinical #islets of Langerhans #isolation #transplantation #medicine #biology #diabetes #IDDM #laboratory #research #cure #Leiden University #LUMC #dissertation #PhD #Doctor of Philosophy #publisher #scan #ebook #book #ISBN #9789080216402 #9789080216419 #Holland #Netherlands #TakeNode #1Memo #michelvanderburg