PentaStarch UWS Pancreatic Islet Purification • 20240802

Following first introduction of the use UWS (the UW organ preservation solution) for pancreatic islet isolation in 1989 , next also purification of the islets in UWS was presented in 1991, with two options.
One option was an iso-osmotic density gradient solution of Percoll in UWS. The second option was a density gradient solution created by increasing the Pentastarch concentration in UWS (7) . Note that Pentastarch is a component of UWS.

The Percoll-UWS poster was posted recently (8)
Here the remastered poster presentation on Pentastarch-UWS :
Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

The poster was designed and produced on my personal Macintosh computer and Imagewriter dotmatrix printer, the night of June 3-4, 1991, using the MacDraw II application.
The Apple Imagewriter-II matrix printer, allows printing landscape across a chain of several sheets of paper, strips, the width of the poster (here 3 sheets long). The strips of the poster (here 3 strips) were then glued on site along the perforation, creating the 36/24 inch poster.
Finally the photo print of the purified islets was pasted in the poster at the spot at the venue in Lyon. In Lyon a photo of a dithizone stained Pentastarch purified islet sample was shown. To save time the archive was not searched for that image, and another image of Pentastarch purified islets was inserted in this remastered edition, a reflection contrast micrograph of an ultrathin section of Pentastarch purified islets immuno-stained with gold on insulin in the islet cells.

Nowadays, it is a challenge opening such old Mac files … Luckily, I was able to open this MacDraw II poster file in LibreOffice Vanilla and now learned to use the app to further correct most conversion issues. Pixelmator Pro was used for additional ‘repair’ and insertion of the photo scan.

Notes

1. Minneapolis 1989 Poster UW Solution Islet Isolation | 20220822 | Michel van der Burg | michelvanderburg•com | URL https://michelvanderburg.com/2022/08/22/minneapolis-1989-poster-uw-solution-islet-isolation-20220822/

2. Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

3. Van der Burg MPM, Guicherit OR, Ploeg RJ, Scherft JP, Frölich M, Gooszen HG. Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

4. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Gooszen HG. Sigma’s industrial grade dextran is not suitable for islet isolation. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

5. Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. CCK and GIP control of canine isolated islet function. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

6. Pancreatic islet transplantation Thesis Repository Leiden University | 20221125 | Michel van der Burg | Miracles•Media | URL https://michelvanderburg.com/2022/11/25/pancreatic-islet-transplantation-thesis-repository-leiden-university-20221125/

7. Pentastarch is the synthetic colloid component of UWS (ViaSpan / Belzer solution), a purified fraction (250 kD) of hydroxyethyl starch. Pentastarch powder was used here to increase the Pentastarch concentration and density of the UWS.

8. Percoll UWS Pancreatic Islet Purification • 20240731 | Michel van der Burg | michelvanderburg•com

Citation info : PentaStarch UWS Pancreatic Islet Purification • 20240802 | Michel van der Burg | michelvanderburg•com | TakeNode d11f1cef-a343-4466-8814-3e52a0261cae

Percoll UWS Pancreatic Islet Purification • 20240731

Lyon 1991 Percoll Poster | 20240731 • 20240808 | Michel van der Burg | michelvanderburg•com | TakeNode 8cd3161a-2405-4586-aaeb-08096e1a636e

Here one of these poster presentations, on Percoll-UWS :
Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

The poster was designed and produced on my personal Macintosh computer and Imagewriter dotmatrix printer, the night of June 3-4, 1991, using the MacDraw II application, inserted with a bar graph prepared in the Cricket Graph app.
The Apple Imagewriter-II matrix printer, allows printing landscape across a chain of several sheets of paper, strips, the width of the poster (here 3 sheets long). The strips of the poster (here 5 strips) were then glued on site along the perforation, creating a 36/41 inch poster.
Finally the RCM photo print of the purified human islets was pasted in the poster at the spot at the venue in Lyon.

Nowadays, it is a challenge opening such old Mac files … Luckily, I was able to open this MacDraw II poster file in LibreOffice Vanilla (open source app) with nice results. Only minor issues appeared with text spacing of the top row of the table , and the caption in the photo section. Some color is showing in the graphs , not evident on my grayscale equipment in the 90s. …. The poster image of 20240731 has been replaced by a remastered image , see Note Update 20240808 below.

Notes

Update 20240808

Remastered poster ‘Lyon 1991 Percoll Poster | 20240731 • 20240808’

Following the first publication here last month (20240731) of the original 1991 MacDraw poster opened (converted) in LibreOffice Vanilla (1), I learned how to do some editing in the LibreOffice app to further restore poster layout details such as line spacing, and font style. An archive photo of another poster presentation in Pittsburgh in 1992 (that will be posted here soon) guided this restoration.

Current workflow :
1) opening the MacDraw II file in LibreOffice Vanilla
2) editing in in LibreOffice Vanilla , saving as LibreOffice Vanilla file (OpenDocument Drawing – extension odg).
3) opening the photo tiff image in the Mac’s Preview app, and copying the displayed image to the clipboard
4) pasting the clipboard image in the LibreOffice doc , and dragging to adjust size and location
5) exporting the file as PDF (image resolution 300 DPI ; PDF/A-1b ; PDF/UA).
6) Converting the PDF in the Preview app to TIFF 144 pixels/inch.
7) Adding caption , metadata etc with JPEG conversion in PhotoMill.

This 20240731 post now has this updated 20240808 – poster image.

Citation info : Percoll UWS Pancreatic Islet Purification • 20240731 | Michel van der Burg | michelvanderburg•com

Stefan & Ursula Breslauer in Westerborkfilm | 20240305

Filmmaker Rudolf Breslauer also filmed two of his children in the Westerborkfilm…

Stefan (left) & Ursula Breslauer, children of Rudolf Breslauer, the filmmaker of the Westerbork film at the farm of Camp Westerbork in 1944 – identified by the dutch photographer Sake Elzinga, who received Breslauer’s family photo albums last year when the family of Ursula – the only survivor – visited an expo on Breslauer in the Westerbork museum in the Netherlands.

Camp commander (SS-Obersturmführer) Albert Gemmeker ordered the Westerbork film , made by the German Jewish prisoner, photographer, Rudolf Breslauer in the spring of 1944.

Today 80 years ago – March 5, 1944 – the camp is an ‘Arbeitslager’ – a work camp – when Rudolf Breslauer starts filming the daily life of the Westerbork prisoners — inside : in the barracks, for example a religious service, cabaret, workshops, factories, aircraft and battery recycling, medical care, and outside the barracks : construction of a greenhouse, a football match, women working out, chopping wood, incoming transports, and eventually also the departure of a deportation train. After Breslauer films the deportation of Jews, Roma and Sinti to Bergen-Belsen and Auschwitz on May 19, 1944 the filming stops.
The haunting image of the 9-year-old dutch Sinti-girl Settela, standing in the closing doors of the goods train, and the unique footage of that deportation train that leaves the Westerbork camp, became iconic after the war.

Deportation Breslauer family

Werner Rudolf Breslauer , his wife Bella Weihsmann, sons Stefan and Max Michael (Mischa), and daughter Ursula were deported autumn 1944 from Westerbork to Theresienstadt and Auschwitz. Only Ursula survived.

Stefan & Ursula Breslauer in Westerborkfilm | 20240305 | Settela•Com | Frame 127475 from Westerbork Film 🎦 2021 | 20220302 | Settela•Com | ISSN 2949 9313 | Footage filmed by Rudolf Breslauer in 1944, courtesy of NIOD | Sound and Vision

Notes

Clip from : Westerbork Film 🎦 2021 | Complete Remastered Edition | YouTube https://youtu.be/ZiLNDziwEtc

Westerbork Film 🎦 2021 | 20220302 | Settela•Com | URL https://settela.com/2022/03/02/westerbork-film-🎦-2021-complete-remastered-edition-20220302/

Kinderen van filmmaker Breslauer herkend in historische Westerborkfilm. Dutch national news broadcaster NOS Nieuws, in cooperation with RTV Drenthe, 13:18 Monday 4 March 2024 | URL https://nos.nl/artikel/2511414-kinderen-van-filmmaker-breslauer-herkend-in-historische-westerborkfilm

Scene with Stefan & Ursula Breslauer, starting at 56:13 in the 1986 RVD edition of the Westerborkfilm:
Stefan & Ursula Breslauer in Westerbork Film RVD | 20240305 | Settela•Com | URL https://youtube.com/clip/UgkxfNzA72JeGgVoOFp_VTI4EQQr3yTwXu6_

Settela Film | 20220630 | Michel van der Burg | Settela•Com

Deportation Westerbork Film | 20210719 | Michel van der Burg | Settela•Com

English introduction to Westerborkfilm :
Westerborkfilm Introduction | 20220507 | Michel van der Burg | Settela•Com | URL https://settela.com/2022/05/07/westerborkfilm-introduction-20220507/

Citation info : Stefan & Ursula Breslauer in Westerborkfilm | 20240305 | Settela•Com | ISSN 2949-9313 | URL https://settela.com/2024/03/05

Deportation | 20240226

Camp commander (SS-Obersturmführer) Albert Gemmeker oversees the deportation of Jews, Sinti, and Roma from the Westerbork transit camp May 19, 1944 .

Gemmeker is the producer of the Westerborkfilm — he ordered the film. Cameraman is the German Jewish prisoner Rudolf Breslauer.

Source : Frame 2608 from Westerborkfilm Introduction | 20220507 | Settela•Com | ISSN 2949-9313 | URL https://settela.com/2022/05/07/westerborkfilm-introduction-20220507/

‘Entartete Art’ 1935 – Coleman Hawkins & Leo de la Fuente in Holland | 20231123

‘Entartete Art’ 1935 – Coleman Hawkins & Leo de la Fuente in Holland

American saxophonist Coleman Hawkins announces and plays – accompanied by Leo de la Fuente on piano – on his tenor saxophone ‘I wished that I were twins’.

In 1934, Coleman Hawkins left the Fletcher Henderson Orchestra and Amerika, moved to Europe, and joined the Jack Hylton Orchestra in England. Hylton and his band made regular ‘continental’ tours, and started another European tour January 1935 accompanied by Coleman Hawkins in Holland. At the end of January 1935 Hawkins joins the dutch band The Rambers … for 8 days … because Hawkins was denied entry to Germany because of his race, while Hylton and his band continued their tour without him and play for eight days at the Berlin Philharmonic Hall.

To end this special week with Hawkins well, the bandleader of The Ramblers – Theo Uden Masman – arranged with Decca for recordings 4 february 1935 in Pulchri Studio in The Hague, Holland, including this : I wish I were twins…that was also recorded on film by Polygoon (Polygoontoon) for the dutch cinema news for next week .

Coleman Hawkins is accomponied here on film by the dutch jazz pioneer Leo de la Fuente on piano , playing ‘I wished that I were twins’ . After the recordings, Hawkins moves further into Europe.

Leo – Leonard Henriques – de la Fuente, who was born Jewish in Amsterdam 28 March 1902, was deported by the nazi’s to Auschwitz on 2 November 1942, and died 30 April 1944 ‘somewhere in Mid Europe’.

entartete art jazz 1935 coleman hawkins leo de la fuente in holland 20231123 1

entartete art jazz 1935 coleman hawkins leo de la fuente in holland 20231123 2

entartete art jazz 1935 coleman hawkins leo de la fuente in holland 20231123 3

References

About Leonard Henriques de la Fuente, his wife Hendrika de Vries and his daughters Benvenida and Mietje. Joods Monument. URL https://www.joodsmonument.nl/en/page/680262/about-leonard-henriques-de-la-fuente-his-wife-hendrika-de-vries-and

Biografie Leo de la Fuente. Muziekencyclopedie.nl | Beeld & Geluid. URL https://www.muziekencyclopedie.nl/action/entry/Leo+de+la+Fuente

The Hawk in Holland. Jacky de Vries. Theater Sentiment – De verhalen over de artiesten van weleer – Jaargang 3 Nummer 4. URL https://theatersentiment.nl/the-hawk-in-holland/

Jack Hylton 1892-1965. Abaigh McKee. Politics & Propaganda | Music and the Holocaust | URL https://holocaustmusic.ort.org/politics-and-propaganda/jack-hylton/

Film edited from source (video footage) : Polygoon Hollands Nieuws (Polygoontoon, producer) dutch cinema news reel week 7 , 1935 | Sound & Vision (Open Images)

Citation : ‘Entartete Art’ 1935 – Coleman Hawkins & Leo de la Fuente in Holland | 20231123 | Settela•Com | ISSN 2949-9313 | TakeNode 4f388407-5ccc-4816-aef6-6064fcee35b2

TAGS #Coleman #Hawkins #Leo #Fuente #music #jazz #Entartete #art #Europe #tour #tournee #Berlin #Germany #tenor #sax #saxophon #saxofoon #interbellum #Hawk #Holland #The #Hague #Pulchri #Studio #Netherlands #Decca #art #black #Jew #racism #diversity #nazi #politics #propaganda #holocaust #Polygoon #Polygoontoon #film #cinema #news #theater #band #pioneer #bandleader #Entartete #Kunst #degenerate #art #Jack #Hylton #orchestra #twins #Sound #Vision #broadcast #performance #history #England #1Memo #michelvanderburg #MiraclesMedia #SettelaCom

French Refugees October 1918 in Holland | 20231031

Silent film — Towards the end of World War I – in the summer 1918 , after the Allies defeated the Germans in the Second Battle of the Marne – large groups of Belgians and French refugees flee in front of the retreating enemy force to the neutral Netherlands.

The Dutch army and Red Cross prepared to receive a possible 100,000 evacuees in the border towns in the south of the Netherlands.

After wandering weeks in the north of France , and next crossing Belgium on foot, the first group of French refugees arrived at the Dutch border October 20, completely exhausted.

At the gate in the electric wire fence at the border to the Netherlands – the so-called ‘Death Wire’ (dutch : Dodendraad ) Dutch soldiers took them over from German escorts.

Some refugees had travelled by tram part of the route crossing Belgium to the belgian border town Molenbeersel.

At the dutch border the refugees were welcomed and ladies from relief committees provide the refugees with food and drinks on the road to Stramproy in the Netherlands.

Within days , thousands of French refugees arrive in the village of Stramproy and are transferred to the city of Weert .

On arrival in the city center of Weert the dutch army registered people and handed out soup and bread.

The weak, the sick, and maternity women were cared for by the Red Cross and taken to an aid post for further care.

The refugees were sheltered the night in buildings like convents and schools in Weert, before being housed elsewhere in the country the following days.

References

Source (video footage) : Belgische vluchtelingen 1e Wereldoorlog. Open Images | Beeld & Geluid (Sound &Vision).

Weert en het einde van W.O.-I. | Jan Henkens | Stichting Historisch Onderzoek Weert | URL http://www.showeert.nl

Noord-Franse vluchtelingen in Weert 1918 in Den Grooten Oorlog | Grevenbroekmuseum vzw | URL https://www.grevenbroekmuseum.be

Citation : French Refugees October 1918 in Holland | 20231031 | Settela•Com | ISSN 2949-9313 | TakeNode bf10307b-fc98-4d67-ad22-82ef8cb3fddd

TAGS #french #refugee #train #tram #steam #horse #woman #WorldWarI #WW1 #Weert #Molenbeersel #Stramproy #Germany #France #Belgium #Netherlands #Holland #Europe #border #electric #wire #Dodendraad #death #war #peace #fence #shelter #flag #flight #evacuation #Red #Cross #army #help #welcome #food #drink #soup #jenever #walk #battle #Marne #1Memo #michelvanderburg #MiraclesMedia #SettelaCom

Sara & Me • Selfie For The Sea | 20160608 • 20230907

Short film that I made June 8, 2016 as ‘Selfie For The Sea’ for World Oceans Day. My encounter with Sara the southern Sea lion diving in the open Caribbean Sea along the coast of Curaçao (Netherlands) was filmed by Gordy Cox in 2004.
Remastered 20230907 edition of vimeo•com/169906000 posted 20160608 . @CelebrateOceans @WorldOceansDay
Citation : Sara & Me • Selfie For The Sea | 20160608 • 20230907 | Michel van der Burg | 1-memo•com | TakeNode 7eb3c7e0-f4fb-48aa-8dbe-3363eef0e6bc

TAGS #SelfieForTheSea #WorldOceansDay #sea #lion #sealion #Sara #Netherlands #Curaçao #Caribbean #dive #water #mammal #encounter #selfie #ocean #splash #climate #remaster #vimeo #1Memo #michelvanderburg