Percoll UWS Pancreatic Islet Purification (20240731) now has a remastered poster image , see below.
Lyon 1991 Percoll Poster | 20240731 • 20240808
20240808 – remastered poster : Following the first publication here last month (20240731) of the original 1991 MacDraw poster opened (converted) in LibreOffice Vanilla (1), I learned how to do some editing in the LibreOffice app to further restore poster layout details such as line spacing, and font style. An archive photo of another poster presentation in Pittsburgh in 1992 (that will be posted here soon) guided this restoration.
Current workflow : 1) opening the MacDraw II file in LibreOffice Vanilla 2) editing in in LibreOffice Vanilla , saving as LibreOffice Vanilla file (OpenDocument Drawing – extension odg). 3) opening the photo tiff image in the Mac’s Preview app, and copying the displayed image to the clipboard 4) pasting the clipboard image in the LibreOffice doc , and dragging to adjust size and location 5) exporting the file as PDF (image resolution 300 DPI ; PDF/A-1b ; PDF/UA). 6) Converting the PDF in the Preview app to TIFF 144 pixels/inch. 7) Adding caption , metadata etc with JPEG conversion in PhotoMill.
The 20240731 post now has this updated 20240808 – poster image.
Citation info : Lyon 1991 Percoll Poster | 20240731 • 20240808 | Michel van der Burg | michelvanderburg•com | TakeNode 8cd3161a-2405-4586-aaeb-08096e1a636e
Following first introduction of the use UWS (the UW organ preservation solution) for pancreatic islet isolation in 1989 , next also purification of the islets in UWS was presented in 1991, with two options.
One option was an iso-osmotic density gradient solution of Percoll in UWS. The second option was a density gradient solution created by increasing the Pentastarch concentration in UWS (7) . Note that Pentastarch is a component of UWS.
The Percoll-UWS poster was posted recently (8)
Here the remastered poster presentation on Pentastarch-UWS :
Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
The poster was designed and produced on my personal Macintosh computer and Imagewriter dotmatrix printer, the night of June 3-4, 1991, using the MacDraw II application.
The Apple Imagewriter-II matrix printer, allows printing landscape across a chain of several sheets of paper, strips, the width of the poster (here 3 sheets long). The strips of the poster (here 3 strips) were then glued on site along the perforation, creating the 36/24 inch poster.
Finally the photo print of the purified islets was pasted in the poster at the spot at the venue in Lyon. In Lyon a photo of a dithizone stained Pentastarch purified islet sample was shown. To save time the archive was not searched for that image, and another image of Pentastarch purified islets was inserted in this remastered edition, a reflection contrast micrograph of an ultrathin section of Pentastarch purified islets immuno-stained with gold on insulin in the islet cells.
Nowadays, it is a challenge opening such old Mac files … Luckily, I was able to open this MacDraw II poster file in LibreOffice Vanilla and now learned to use the app to further correct most conversion issues. Pixelmator Pro was used for additional ‘repair’ and insertion of the photo scan.
2. Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
3. Van der Burg MPM, Guicherit OR, Ploeg RJ, Scherft JP, Frölich M, Gooszen HG. Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
4. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Gooszen HG. Sigma’s industrial grade dextran is not suitable for islet isolation. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
5. Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. CCK and GIP control of canine isolated islet function. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
7. Pentastarch is the synthetic colloid component of UWS (ViaSpan / Belzer solution), a purified fraction (250 kD) of hydroxyethyl starch. Pentastarch powder was used here to increase the Pentastarch concentration and density of the UWS.
8. Percoll UWS Pancreatic Islet Purification • 20240731 | Michel van der Burg | michelvanderburg•com
Citation info : PentaStarch UWS Pancreatic Islet Purification • 20240802 | Michel van der Burg | michelvanderburg•com | TakeNode d11f1cef-a343-4466-8814-3e52a0261cae
Lyon 1991 Percoll Poster | 20240731 • 20240808 | Michel van der Burg | michelvanderburg•com | TakeNode 8cd3161a-2405-4586-aaeb-08096e1a636e
Following first introduction of the use UWS (the UW organ preservation solution) for pancreatic islet isolation in 1989 , next also purification of the islets in UWS was presented in 1991, with two options. One option was an iso-osmotic density gradient solution of Percoll in UWS. The second option was a density gradient solution created by increasing the Pentastarch concentration in UWS . Note that Pentastarch is a component of UWS.
Here one of these poster presentations, on Percoll-UWS : Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
The poster was designed and produced on my personal Macintosh computer and Imagewriter dotmatrix printer, the night of June 3-4, 1991, using the MacDraw II application, inserted with a bar graph prepared in the Cricket Graph app. The Apple Imagewriter-II matrix printer, allows printing landscape across a chain of several sheets of paper, strips, the width of the poster (here 3 sheets long). The strips of the poster (here 5 strips) were then glued on site along the perforation, creating a 36/41 inch poster. Finally the RCM photo print of the purified human islets was pasted in the poster at the spot at the venue in Lyon.
Nowadays, it is a challenge opening such old Mac files … Luckily, I was able to open this MacDraw II poster file in LibreOffice Vanilla (open source app) with nice results. Only minor issues appeared with text spacing of the top row of the table , and the caption in the photo section. Some color is showing in the graphs , not evident on my grayscale equipment in the 90s. …. The poster image of 20240731 has been replaced by a remastered image , see Note Update 20240808 below.
2. Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
3. Van der Burg MPM, Guicherit OR, Ploeg RJ, Scherft JP, Frölich M, Gooszen HG. Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
4. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Gooszen HG. Sigma’s industrial grade dextran is not suitable for islet isolation. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
5. Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. CCK and GIP control of canine isolated islet function. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.
Following the first publication here last month (20240731) of the original 1991 MacDraw poster opened (converted) in LibreOffice Vanilla (1), I learned how to do some editing in the LibreOffice app to further restore poster layout details such as line spacing, and font style. An archive photo of another poster presentation in Pittsburgh in 1992 (that will be posted here soon) guided this restoration.
Current workflow : 1) opening the MacDraw II file in LibreOffice Vanilla 2) editing in in LibreOffice Vanilla , saving as LibreOffice Vanilla file (OpenDocument Drawing – extension odg). 3) opening the photo tiff image in the Mac’s Preview app, and copying the displayed image to the clipboard 4) pasting the clipboard image in the LibreOffice doc , and dragging to adjust size and location 5) exporting the file as PDF (image resolution 300 DPI ; PDF/A-1b ; PDF/UA). 6) Converting the PDF in the Preview app to TIFF 144 pixels/inch. 7) Adding caption , metadata etc with JPEG conversion in PhotoMill.
This 20240731 post now has this updated 20240808 – poster image.
Citation info : Percoll UWS Pancreatic Islet Purification • 20240731 | Michel van der Burg | michelvanderburg•com
JR: Chronicles • LOTTE Museum of Art, Seoul, South Korea • May 3rd – August 6th, 2023
After its inaugural exhibition at the Brooklyn Museum in New York (1), and a European tour (2) at the Saatchi Gallery in London (UK), the Groninger Museum in the Netherlands (3), and next in Germany at the Kunsthalle in Munich, JR: Chronicles is now for the first time in Asia at the LOTTE Museum of Art in Seoul (South Korea).
The INSIDE OUT installation in the JR: Chronicles exhibition is screening our original film on Brussels’ Afghan refugees that was published first online for World Refugee Day June 20th 2016 : Béguinage Refugees Into The Wild (4) . This film wasn’t replaced by the special exhibition edition (with updated credits) we offered (Sep 2019) for the premiere of JR: Chronicles in the Brooklyn Museum. We later did publish that new 4K edition online , entitled “Béguinage Refugees Into The Wild ~ JR: Chronicles Edition” (5).
Post reference JR: Chronicles LOTTE Museum Seoul | 20230716 | Miracles•Media
Filmography
1. JR: New York Show – Afghan Refugees Inside Out Belgium. URL michelvanderburg.com/2020/02/06 2. JR Groninger Show – Afghan Refugees Inside Out . URL michelvanderburg.com/2022/01/07 3. JR: Chronicles Groninger Museum. URL michelvanderburg.com/2023/03/01 4. Béguinage Refugees Into The Wild. URL michelvanderburg.com/2016/06/20 . 5. Béguinage Refugees Into The Wild ~ JR: Chronicles Edition. URL michelvanderburg.com/2020/02/05
Short impression of visiting JR: Chronicles in the Groninger Museum in Groningen, The Netherlands — a major show of the work of the artist JR, organized by the Brooklyn Museum. Here focusing on the video wall of Inside Out projects, screening our short film Béguinage Refugees Into The Wild.
The exhibition is touring the world. First , the Brooklyn Museum in New York from October 4, 2019 – May 3, 2020. Next, the Saatchi Gallery in London from June – Oct 2021. And more recent the Groninger Museum in Holland (The Netherlands) from Nov 2021 untill June 2022 — filmed here June 2022. A promotional film with images of all these places was posted 20220107.
The show highlights JR’s global public participatory art project INSIDE OUT ( @insideoutproject ) .
A video wall is screening the Best of INSIDE OUT videos – including our film ‘Béguinage Refugees Into The Wild’ – a short documentary on the Inside Out project “Justice for Afghan refugees in Belgium”.
Our short film documents the Afghan refugees living in the Brussels Béguinage church , with music by Félix Snyers on the Béguinage church pipe organ, and their portrait’s (posters) by Chiara Ravano for the Inside Out project “Justice for Afghan refugees in Belgium” exhibition at Olivier Bonny’s workshop in Salon Mommen, Brussels, Belgium, March 2014. A Beguinage Project report by Kristen Cattell & Michel van der Burg.
License info JR: Chronicles Groninger Museum | 20230301 | Miracles•Media | TakeNode fbe93b03-5b53-4b3f-92ce-df537ffc1d96
