Author Proof Edition • Miracles.Media • 20241228_1 • TakeNode 7118abf6-259c-494c-8445-879afc6d862c
Below the update today added in the 20241224 post : Pancreatic Islet Isolation With UW Solution • A New Concept • Michel van der Burg • Miracles.Media • @1MEMO 20241224 • URL https://michelvanderburg.com/2024/12/24/pancreatic-islet-isolation-with-uw-solution-a-new-concept-20241224/
As reported in our poster and the manuscript delivered in Barcelona of our paper ‘Pancreatic Islet Isolation With UW Solution: A New Concept’ we had been using collagenase in UW solution in this first short (n=4) series of experiments.
Please note, that I changed the description of the collagenase solution to Hanks’ solution (HBBS) when editing the author proof edition shortly before publication of the final paper August 1990 (PMID_2202137 ; 14) — to best convey our current insights and practice.
For hypothetical reasons only — because the UWS was expected to be detrimental perhaps to cell viablity at the higher temperatures during collagenase digestion of the pancreas (the first stage of islet isolation) — I decided to change the collagenase solution in June 1989 when I began a new series of islet isolations for our first canine islet transplants. From that point on, a modified Hanks’ solution was used for collagenase (17).
We did not observe a difference at the time , whether using one or the other solution for collagenase digestion , with respect to either islet purity, recovery, or viability at islet isolation and days later in culture.
Years later , again tor hypothetical reasons , collagenase in UWS was chosen for the isolation of pig islets, in experiments with a long cold ischemia time of the pancreas during transport with distant pancreas procurement from sows in a slaugtherhouse — Ballering (distant family) in Son — near the south border of The Netherlands, with injection of cold collagenase solution before transport, with the aim of better preservation of the islets (18).
Notes
17) Michael P.M. van der Burg. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation. (Doctoral Thesis, Faculty of Medicine, Leiden University Medical Center (LUMC) , Leiden University), Boskoop: M.P.M. van der Burg | Miracles.Media (ISBN electronic, pdf, 9789080216402 ; ISBN print 9789080216419, 9080216410), 1994: 192 p. Leiden University https://catalogue.leidenuniv.nl/permalink/31UKB_LEU/18s393l/alma9940161419602711
18) Van der Burg MPM, Graham JM. Iodixanol density gradient preparation in University of Wisconsin solution for porcine islet purification. ScientificWorldJournal. 2003 Dec 1;3:1154-9. doi: 10.1100/tsw.2003.107. PMID: 14646009; PMCID: PMC5974767.
Citation info : Author Proof Edition • Michel van der Burg • Miracles.Media • @1MEMO 20241228 • URL michelvanderburg.com/2024/12/28/
