Viability of Fresh vs Cultured Pig Islets for Transplants • Cell Transplant Society 1999 Montreux • 20241204


Oral presentation Michel van der Burg, Cell Transplant Society congress 1999 in Montreux, Switzerland

A novel iodixanol density gradient for purification of pancreatic islets was developed in the difficult pig model of islet isolation. Viability of freshly isolated islets is not guaranteed.
After short-term storage of the islets in culture, pig islets restored normal blood sugar levels after xeno transplantation in diabetic mice.


1-Day Cultered Islets (extra slide) • @1MEMO 20241204_1

Slideshow (pdf)

Viability of Fresh vs Cultured Pig Islets for Transplants • Montreux Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20241204_2 • TakeNode e745d625-27c2-4eb8-b4c2-4300623c3303 • File 1MEMO_20241204_2

1MEMO_20241204_2Download

Rijkelijkhuizen JKRA, Bouwman E, Van der Burg MPM. Viability of fresh vs cultured pig islets for transplants. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999

Other Presentations in Montreux

Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Adult pig islet recovery during Liberase isolation, OptiPrep purification and culture for transplantation in nude mice. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999 . The Sydney 1999 presentation of this work has been posted – @1MEMO 20241203

Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Terpstra OT, Ricordi C. Optiprep for human islet purification. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999 . The Igls 1999 presentation of this work has been posted – @1MEMO 20241202

Update 20260319 : Vimeo video replaced by YouTube copy

Citation info : Viability of Fresh vs Cultured Pig Islets for Transplants • Cell Transplant Society 1999 Montreux • Michel van der Burg • Miracles.Media • @1MEMO 20241204 • TakeNode 3ba1c894-eb71-4c3f-89aa-198c48cd480c • URL michelvanderburg.com/2024/12/04/

Iodixanol Purification of Porcine Islets • Sydney IPITA 1999 • 20241203

Slides oral presentation Michel van der Burg, IPITA congress 1999 in Sydney, Australia

A novel iodixanol density gradient for purification of pancreatic islets was developed in the difficult pig model. The pig islets restored normal blood sugar levels after xeno transplantation in diabetic mice.

Slideshow (pdf) oral presentation Michel van der Burg, IPITA congress 1999 in Sydney, Australia

Recovery of adult pig islets during isolation using Liberase vs collagenase-P, purification in OptiPrep-UWS, and culture for transplantation in nude mice • Sydney IPITA 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20241203_2 • TakeNode 5f53ae49-6fb8-4bef-a434-ac2ce8151c2a • File 1MEMO_20241203_2

1MEMO_20241203_2Download

Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Recovery of adult pig
islets during isolation using Liberase vs collagenase-P, purification in OptiPrep-UWS, and
culture for transplantation in nude mice. 7th World congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999

Updates

Update 20260319 : Vimeo video replaced by YouTube copy

Citation info : Iodixanol Purification of Porcine Islets • Sydney IPITA 1999 • Michel van der Burg • Miracles.Media • 1MEMO 20241203 • TakeNode ea1f1abd-6227-4ae6-985b-f8188310cd24 • URL michelvanderburg.com/2024/12/03/

Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • 20241202

Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202_1

I started an archive at Archive.org November 11, 2010 with this slideshow (pdf) of my January 1999 oral presentation in Igls (Innsbruck), Austria, at the 18th Workshop of the AIDSPIT (Artificial Insulin Delivery Systems, Pancreas and Islet Transplantation) Study Group. (Now known as AIDPIT).

The presented results of this innovative islet purification method — developed over nearly 10 years (from 1989) in our Leiden islet laboratory (LUMC) — were the outcome of work I had done spring 1998 with the human islet transplantation group of Camillo Ricordi at the Diabetes Research Institute in Miami , Florida, USA.

Slideshow (pdf) Archive.org

Description

Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999

Slides oral presentation Michel van der Burg, Aidspit meeting 25 Jan 1999, in Igls, Austria

Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification. 18th Workshop Aidspit, Igls (Austria) Jan. 24-26, 1999

Program :
18TH WORKSHOP OF THE AIDSPIT STUDY GROUP – JANUARY 24-26, 1999 – IGLS/AUSTRIA . MONDAY, JAN 25, 1999 – KONGRESSHAUS IGLS, MAIN LECTURE HALL
SESSION 2 – ISLET ISOLATION AND TRANSPLANTATION
CHAIRMEN:K. FEDERLIN, GIESSEN, GERMANY – G. POZZA, MILAN, ITALY 9.00 – 10.30 FREE COMMUNICATIONS
9.45- Efficacy of the novel iodixanol-UWS density gradient for human islet purification.
Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C; Leiden, The Netherlands and Miami, USA

File : mvdb19990118_1534_AIDSPIT.pdf | Uploaded 20101111 and replaced by the same pdf with updated metadata of the document on 20101112 (see Notes section).

Citation info (Archive file) : Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202 • URL https://archive.org/details/EfficacyOfTheNovelIodixanol-uwsDensityGradientForHumanIslet • TakeNode 54cc8b66-672e-4b13-913c-449038ed0521

Video Slideshow

The slideshow video embedded below, was first posted in 2018 as Human Islet Purification Miami 1998 • 20181102

Notes

The slideshow pdf file is available for download at Archive.org. I advise against the other download options presented by Archive.org , which do not correctly represent the slideshow.

The file was also uploaded to Scribd at the same time – URL https://www.scribd.com/document/42197150/Efficacy-of-the-novel-iodixanol-UWS-density-gradient-for-human-islet-purification-michelvanderburg-19990125

Abstract reference

Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification (Abstract). Acta Diabetol 1998; 35: 247.

Citation info (this post) : Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202 • URL michelvanderburg.com/2024/12/02/

Jon Anderson Love and Understanding • ♻1MEMO 20241201

♻1MEMO in Music , Poetry , Motion

Jon Anderson Love and Understanding • A fan found art thing of Akeldama’s found objects • new experiment

Music & Poetry & Motion

I recently found Akeldama’s work ‘recycling’ my 1memo’s in music, poetry, and motion, generally in the intro and outro.

Found art :

♻1MEMO 20210617

Poetry in the intro is a remix with @1MEMO 20210617
Poppy • 20210617 • 1-memo•com • URL https://1-memo.com/2021/06/17/poppy-20210617/ • YouTube https://youtu.be/s3b8-FeBQlQ

♻1MEMO 20230317

The outro is a remix of @1MEMO 20230317
Sloshing Sea | 20230317 | Michel van der Burg | 1-memo•com • URL https://1-memo.com/2023/03/17/sloshing-sea-20230317/ • YouTube https://youtu.be/SAH4NBTBZjw

Citation info : Jon Anderson Love and Understanding • Michel van der Burg • Miracles•Media • ♻1MEMO 20241201

Kate Rusby Little Jack Frost • ♻1MEMO 20241130

♻1MEMO in Music , Poetry , Motion

Kate Rusby Little Jack Frost • A fan found art thing of Akeldama’s found objects.

Music & Poetry & Motion

I recently found Akeldama’s work ‘recycling’ my 1memo’s in music, poetry, and motion, generally in the intro and outro. Some work’s are only found at the younger companion channel ResurrectionArt.

Found art :

♻1MEMO 20210209

Poetry in the intro is a remix with @1MEMO 20210209
Winter Willows • 20210209 • 1-memo•com • URL https://1-memo.com/2021/02/09/winter-willows-20210209/ • YouTube https://youtu.be/2bp-PrqvzI4

♻1MEMO 20210214

Poetry in the outro is a remix with @1MEMO 20210214
White Green Heart of Holland • 20210214 • 1-memo•com • URL https://1-memo.com/2021/02/14/white-green-heart-of-holland-20210214/ • YouTube https://youtu.be/Wcmx2wAELFY

Citation info : Kate Rusby Little Jack Frost • Michel van der Burg • Miracles•Media • ♻1MEMO 20241130

Surprise • 20241129


Herons in the Green Heart of Holland this week.
Sound recorded wireless at the reed with RØDE wireless GO II transmitters packed in a second furry windscreen. Audio processed in Final Cut Pro with audio Gain +24 dB.

Citation info : Surprise • Michel van der Burg • Miracles•Media • @1MEMO 20241129 • TakeNode 7d3396aa-c87a-4627-ba48-5aa5e9e23c1f

Edit 2 Røde Wireless Micro Stereo • Video Review #12 • 20241128


Second audio edit of the video first uploaded two days ago , dd 20241126. Audio processed using Final Cut Compressor to boost Gain, and the Equalization filter to reduce low frequencies. Settings shown at the end of this video.

Further details in the first post on these Røde Wireless Micro recordings dd 20241126.

Best use headphones ;)

Citation info : Edit 2 Røde Wireless Micro Stereo • Video Review #12 • Michel van der Burg • Miracles•Media • @1MEMO 20241128 • TakeNode 8724c61e-ab1a-450e-aebe-7eba1e97669a