Daily Cookbook • @1MEMO 20250209

‘Spruitjessoep’ , dutch Brussels sprout soup, in the Daily Cookbook (Dagkookboek by Marianne Stuit) I bought new — two copies for both me and my friend — in 1971-2. I was living in Oegstgeest, Holland, near Leiden, as a student Biology (Biochemistry) at the Leiden University. I vividly remember, sliding across the floor (with a sprained ankle) between my tiny kitchen and student room – in the attic of my landlady’s house – with a large enamel pot of Brussels sprout soup.

Citation info : Daily Cookbook • Michel van der Burg • Miracles.Media • @1MEMO 20250209 • TakeNode 092c41bb-18f0-4430-adb4-74b66b1c6933

Sunny • @1MEMO 20250206


Little wild flowers today, walking in the woods of the Green Heart of Holland

Citation info : Sunny • Michel van der Burg • Miracles•Media • @1MEMO 20250206 • TakeNode fe40edcc-13eb-4748-8d46-ddb7804d4f4a

Milan Papers • @1MEMO 20250205

Manuscripts (Preprints) and Abstracts copies of the presentations at the IPITA 1997 meeting in Milan (Italy).

1) Van der Burg MPM, Basir I, Zwaan RP, Bouwman E. Porcine islet preservation during isolation in University of Wisconsin solution. Transplant Proc. 1998 Mar;30(2):360-1. doi: 10.1016/s0041-1345(97)01307-9. PMID: 9532079.

PMID_9532079_20250205_1 • Michel van der Burg • Miracles.Media • @1MEMO 20250205_1 • CC BY-NC-ND • TakeNode 406dbe1e-66e3-4edc-acb8-83fdc8ddc5c8

2) Van der Burg MPM, Basir I, Bouwman E. No porcine islet loss during density gradient purification in a novel iodixanol in University of Wisconsin solution. Transplant Proc. 1998 Mar;30(2):362-3. doi: 10.1016/s0041-1345(97)01308-0. PMID: 9532080.

PMID_9532080_20250205_2 • Michel van der Burg • Miracles.Media • @1MEMO 20250205_2 • CC BY-NC-ND • TakeNode c60e0743-946a-4950-b156-7e671593e482

3) Abstracts

Van der Burg MPM, Basir I, Bouwman E. No porcine islet loss during density gradient purification in a novel iodixanol in University of Wisconsin solution (Abstract). Acta Diabetol 1997; 34: 101.

Van der Burg MPM, Basir I, Zwaan RP, Bouwman E. Porcine islet preservation during isolation in University of Wisconsin solution (Abstract). Acta Diabetol 1997; 34: 136.

1MEMO_ 20250205_3 • Michel van der Burg • Miracles.Media • @1MEMO 20250205_3 • CC BY-NC-ND • TakeNode e5b1c598-fc48-4f2a-93ae-526cd4be082e

Citation info : Milan Papers • Michel van der Burg • Miracles.Media • @1MEMO 20250205 • URL michelvanderburg.com/2025/02/05/

Human Islet Isolation • DRI Miami 1998 • @1MEMO 20250204

Summer 1997, Onno Terpstra, the head of our Surgery Department, asked me to explore our options for starting clinical islet isolation and transplantation in our center – the Leiden University Medical Center (LUMC), and prepare a roadmap.

September 1997, at the IPITA congress in Milan (1) , I learned from Camillo Ricordi, the scientific director of the Diabetes Research Institute (DRI) in Miami – where many guest collaborators work – that visiting the DRI some weeks might be a good option to gain sufficient first work experience in his clinical islet transplantation group.

December 1997, with a ‘Roadmap’, I got the green light to start the operationalization of clinical islet transplantation in the LUMC, first with a working visit to Miami to study the state of the art ‘Ricordi’ method of human islet isolation, and then start the human islet isolation work in our Surgery lab in Leiden.

In 1990 I had already gained some initial research experience in our Leiden Islet Laboratory with a small number of isolations of islets from human donor organs, using our innovative method of density gradient purification based on the UW solution (2).

During the first three months of 1998 I worked intensively with my student colleague Josephine Rijkelijkhuizen so that she would be able to continue our xenotransplantation research project as independently as possible in our Surgery research team.

April 1998, after participating in the 1st Workshop on Clinical Islet Transplantation in New Orleans (3) – the ideal introduction – a fruitful working visit to the DRI in Miami followed (4,5), with intensive collaboration with the DRI team on, among other things, six human islet isolations, and also the simultaneously (parallel) conducted research into the purification of the human islets in our Iodixanol gradient in UW-Solution (6).

Here a slideshow illustrating this team up with the DRI team in Miami , working on human islet isolation and purification in the innovative Iodixanol-UWS gradient, April-May 1998.

Notes

1) Innovative Islet Purification in Iodixanol-UWS • IPITA 97 • Michel van der Burg • Miracles.Media • @1MEMO 20250128 • TakeNode • URL michelvanderburg.com/2025/01/28/

2) Percoll UWS Purification Pancreatic Islets • ESOT 1991 Talk • Michel van der Burg • Miracles.Media • @1MEMO 20250114 • URL michelvanderburg.com/2025/01/14/

3) Invited participant 1st Workshop on Clinical Islet Transplantation, New Orleans (LA, USA) April 17–19, 1998

4) Working visit April – May 1998 at the “fast track” center of the Diabetes Research Institute (Scientific director Prof. C. Ricordi, MD) of the University of Miami (Miami, FL, USA)

5) DRI Dream Team • Michel van der Burg • Miracles.Media • @1MEMO 20250105 • URL michelvanderburg.com/2025/01/05/

6) Human Islet Purification Miami 1998 • Michel van der Burg • Miracles.Media • 20181102 • URL michelvanderburg.com/2018/11/02/

Citation info : Human Islet Isolation • DRI Miami 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250204 • TakeNode 86e4a319-a769-44d5-8fa5-de2ca90ab63c • URL michelvanderburg.com/2025/02/04/