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Assessment of isolated islet equivalents • Cell Transplant Society 1996 • @1MEMO 20251205


Assessment of isolated islet equivalents • Cell Transplant Society 1996 • Michel van der Burg • Miracles.Media • 1MEMO_20251205_1 • Poster 3rd International Congress Cell Transplant Society, Miami (USA) Sep 29 – Oct 2, 1996


Assessment of isolated islet equivalents • Cell Transplant Society 1996 • Michel van der Burg • Miracles.Media • 1MEMO_20251205_1 • Poster 3rd International Congress Cell Transplant Society, Miami (USA) Sep 29 – Oct 2, 1996 • TakeNode 1300e7cd-85a4-4cd0-9cbc-a88746e625ab • #IEQ #diabetes #islets #transplant #methods #CTRMS

IEQ – Islet Equivalent

IEQ islet stands for Islet Equivalent, a standard unit used to measure the total volume of islets (clusters of insulin cells and other hormone-producing cells in the pancreas). It is defined as a single spherical islet with a diameter of 150 µm. The IEQ is a crucial parameter for assessing the quantity of islets for procedures like clinical islet transplantation, which is a treatment for type 1 diabetes.

Modified international procedure

Following a consensus report in 1989 – 1990 by Ricordi et al (1a, 1b) on islet isolation assessment, the total volume of isolated islets is generally expressed in the number of islet equivalents (IEQs) — defined as islets of 150 µm diameter— and many centers use the advocated now classic international procedure (CIP) of sizing all islets in classes with 50-µm increments (i.e., 50–100, 100–150, etc.) for calculation of the number of IEQs. For each class the number of IEQs are calculated by multiplying the islet counts with a conversion factor — based on the mean volume of that class.

A modified international procedure — based on our conventional Leiden procedure (2,3) with islet sizing in classes with 25-µm increments — is suggested for accurate assessment of the IEQs especially when isolation yields mostly smaller islets, in our paper ‘Assessment of isolated islet equivalents’ presented at the 3rd International Congress Cell Transplant Society, Miami (USA) Sep 29 – Oct 2, 1996, in both a poster (4) , and proceedings paper (5). A photo of the original poster , (retrieved somewhat wrinkled 30 years later from my archive), is posted here, as well as a pdf from the (corrected proof) manuscript of the proceedings paper below (6).

The Cell Transplant Society was founded in 1991, and has been renamed the Cell Transplant and Regenerative Medicine Society (CTRMS).

Notes

1a. Ricordi C, Gray DW, Hering BJ, Kaufman DB, Warnock GL, Kneteman NM, Lake SP, London NJ, Socci C, Alejandro R, et al. Islet isolation assessment in man and large animals. Acta Diabetol Lat. 1990 Jul-Sep;27(3):185-95. doi: 10.1007/BF02581331. PMID: 2075782.

1b. Quantitative And Qualitative Standards For Islet Isolation Assessment In Man And Large Mammals. International Workshop during the 2nd Congress on Pancreas and Islet Transplantation (1989). Camillo Ricordi. Archive Michel van der Burg • Miracles•Media • 1MEMO_20251206_07 . Available from Dec 6, 2025 URL https://michelvanderburg.com/2025/12/06/

2. Van der Burg MPM, Guicherit OR, Frölich M, Scherft JP, Prins FA, Bruijn JA, Gooszen HG. Assessment of islet isolation efficacy in dogs. Cell Transplant. 1994 Jan-Feb;3(1):91-101. doi: 10.1177/096368979400300113. PMID: 8162296.

3. Paper (Notes 2) included as Chapter 3 in my doctoral thesis (Leiden University, November 1994) : Assessment of islet isolation efficacy in dogs. In : Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation ( Chapter 3). (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Nov 24th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 . Direct link to file Chapter 3 , URL https://scholarlypublications.universiteitleiden.nl/access/item%3A3486610/view

4. Van der Burg MPM, Scheringa M, Basir I, Bouwman E. Assessment of isolated islet equivalents. Poster presented at the Third Int. Congress Cell Transplant Soc., Miami (USA) Sept. 29 – Oct. 2, 1996.
In : Assessment of isolated islet equivalents • Cell Transplant Society 1996 • Michel van der Burg • Miracles.Media • @1MEMO 20251205 • URL https://michelvanderburg.com/2025/12/05 • File 1MEMO_20251205_1 • TakeNode 1300e7cd-85a4-4cd0-9cbc-a88746e625ab

5. Van der Burg MPM, Scheringa M, Basir I, Bouwman E. Assessment of isolated islet equivalents. Transplant Proc. 1997 Jun;29(4):1971-1973. doi: 10.1016/s0041-1345(97)00188-7. PMID: 9193483.

6. Manuscript (corrected proof for Transplant Proc paper PMID: 9193483). Van der Burg MPM, Scheringa M, Basir I, Bouwman E. Assessment of isolated islet equivalents • 1MEMO_20251205_2 • TakeNode 48380f3c-b3f7-416e-90bb-ad6c53129e46 • PDF available below , File 1MEMO_20251205_2

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#IEQ #diabetes #islets #transplant #methods #CTRMS

Citation info : Assessment of isolated islet equivalents • Cell Transplant Society 1996 • Michel van der Burg • Miracles.Media • @1MEMO 20251205 • TakeNode 1300e7cd-85a4-4cd0-9cbc-a88746e625ab / 48380f3c-b3f7-416e-90bb-ad6c53129e46 • URL https://michelvanderburg.com/2025/12/05

GLP-1 blood sugar regulation in islet transplants • AIDSPIT 1994 • @1MEMO 20251114

Poster 13th Workshop of the AIDSPIT Study Group , Igls (Austria) January 23–25, 1994• Digitally remastered by author, publisher, M.P.M. van der Burg • Miracles•Media • 20251114 • TakeNode 810cc881-d084-4d2e-b252-d432918b1946 • #GLP1

GLP-1 blood sugar regulation in islet transplants

GLP-1 (glucagon-like peptide-1) is now well-known from the anti-diabetic drugs that mimic the action of this natural hormone GLP-1, to treat type 2 diabetes and obesity. They work by stimulating insulin release, reducing glucagon secretion, slowing stomach emptying, and suppressing appetite, which leads to lower blood sugar and weight loss.

May 1992 we were the first to show direct potentiation of the insulin response from isolated islets by a (near-)physiological dose of the gut hormone GLP-1 during perifusion (in vitro) of canine islets at 7.5-10 mM glucose levels (1,2).

Jan 1994 we next showed this incretin effect of GLP-1 also after islet transplantation (in vivo, in dogs) in this poster (3) and abstract (4) presented at the 13th Workshop of the AIDSPIT (aka AIDPIT) Study Group , Igls, Austria, January 23–25, 1994.

Background of the work was posted earlier this year (5) and the work was published Nov 1994 in my PhD thesis Pancreatic islet transplantation , available online (6).

Notes

1) Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Terpstra JL, Gooszen HG. Function after intrasplenic transplantation of islets of Langerhans in dogs (translated from dutch: Functie na intralienale transplantatie van eilandjes van Langerhans in de hond). Surgeon days ’92 of the Dutch Surgery Society, Rotterdam (The Netherlands) May 21–22, 1992. In : Gut factors controlling pancreatic islets • 20240910 | Michel van der Burg | michelvanderburg•com URL https://michelvanderburg.com/2024/09/10/gut-factors-controlling-pancreatic-islets-20240910/

2) van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Pept. 1995 Dec 7;60(1):61-7. doi: 10.1016/0167-0115(95)00122-4. PMID: 8747785.

3) Metabolic control after canine islet transplantation. M.P.M. van der Burg, P.T.R. van Suylichem, O.R. Guicherit, J.H.M. van Deijnen, M. Frölich, and H.G. Gooszen (Universities of Leiden & Groningen, The Netherlands). Poster presented at the 13th Workshop of the AIDSPIT Study Group , Igls, Austria, January 23–25, 1994 •

4) Metabolic control after canine islet transplantation. Van der Burg M.P.M., Guicherit O.R., van Suylichem P.T.R., Frölich M., van Deijnen J.H.M. , Gooszen H.G. (Leiden, The Netherlands; Groningen, The Netherlands). Horm metab Res 1994; 26: 63. (Abstract). View/Download file 1MEMO_20251113_2

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5) Background of this work was posted in Dutch Islets Transplant Team • @1MEMO 20250125 • URL https://michelvanderburg.com/2025/01/25/dutch-islets-transplant-team-1memo-20250125/

6) Michael P.M. van der Burg. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation. (Doctoral Thesis, Faculty of Medicine, Leiden University Medical Center (LUMC) , Leiden University), Boskoop: M.P.M. van der Burg | Miracles.Media (ISBN electronic, pdf, 9789080216402 ; ISBN print 9789080216419, 9080216410), 1994: 192 p. URL Thesis Repository Leiden University https://hdl.handle.net/1887/3486604

PM – Recording of the 13th AIDSPIT seminar on lessons from clinical islet transplantation was posted yesterday (7).

7) Clinical Islet Transplantation 13th AIDSPIT • @1MEMO 20251113 • URL https://michelvanderburg.com/2025/11/13/clinical-islet-transplantation-13th-aidspit-1memo-20251113/

#GLP1 #diabetes #islets #transplant #AIDSPIT

Updates

20251127 title updated – insert : AIDSPIT 1994

Citation info : GLP-1 blood sugar regulation in islet transplants • AIDSPIT 1994 • Michel van der Burg • Miracles.Media • @1MEMO 20251114 • TakeNode 810cc881-d084-4d2e-b252-d432918b1946 • URL https://michelvanderburg.com/2025/11/14

Clinical Islet Transplantation 13th AIDSPIT • @1MEMO 20251113


Seminar on lessons from clinical islet transplantation

Chairman K. Federlin (Giessen, Germany) opened the seminar Tuesday, January 25, 1994 and David Scharp began with his lesson from the Center in St. Louis (USA) at the 13th Workshop of the AIDSPIT * Study Group , Igls, Austria, January 23–25, 1994.

Notes


Video image: Iodixanol-UWS purified human islets, stained with dithizone (March 18, 1999) • Michel van der Burg , Leiden Islet Laboratory LUMC .

Second, remastered, edition created from the 1st video edition published 20170208 via Vimeo at 1-memo.com .

* AIDSPIT later known as AIDPIT.

Recorded with Olympus mini-tape dictaphone, and re-recorded from the speaker of the dictaphone.

My presentations at the event

Cell preservation in University of Wisconsin solution during isolation of canine islets of Langerhans. Van der Burg M.P.M., Guicherit O.R., Frölich M., Gooszen H.G. (Leiden, The Netherlands) . Horm metab Res 1994; 26: 63. (Abstract). File 1MEMO_20251113_2

Metabolic control after canine islet transplantation. Van der Burg M.P.M., Guicherit O.R., van Suylichem P.T.R., Frölich M., van Deijnen J.H.M. , Gooszen H.G. (Leiden, The Netherlands; Groningen, The Netherlands). Horm metab Res 1994; 26: 63. (Abstract). File 1MEMO_20251113_2

View/Download file 1MEMO_20251113_2

1MEMO_20251113_2Download

Citation info : Clinical Islet Transplantation 13th AIDSPIT • Michel van der Burg • Miracles.Media • @1MEMO 20251113 • TakeNode 24209f1e-67d5-4540-9115-89efcc6090c9 • URL https://michelvanderburg.com/2025/11/13/clinical-islet-transplantation-13th-aidspit-1memo-20251113/

Apple Log HLG • Wild Fall Flowers • @1MEMO 20251112


New take, now using Apple Log with FCP app (HD 50fps ProRes), at the same spot as 2 days ago, when filming HDR-HLG 4K 60fps with standard camera app on iPhone 16 Pro (@1MEMO 20251110). Edited in HLG (Rec. 2020 HLG) project of FCP. HLG video uploaded at YouTube.
Using Vimeo SDR conversion files for uploads elsewhere – eg :

https://1memo.substack.com

https://pixelfed.social/1memo

#applelog #iphone #HLG

Citation info : Apple Log HLG • Wild Fall Flowers • Michel van der Burg • Miracles•Media • @1MEMO 20251112 • TakeNode e2c63818-db81-42be-b852-5b5b4c567d00