Ductus Pancreaticus

① memo 20190503 ~ Ductus Pancreaticus ~ Human pancreas model with a ‘cut’ showing the pancreatic duct for drainage of the digestive pancreatic juice to the intestinal tract – this week in the Corpus museum in Oegstgeest near Leiden, Holland.
I used that pancreatic duct many years for (retrograde) infusion of digestive enzymes (mostly collagenase) in the pancreas to digest the connective tissue in the gland and release the’isolated’ islets of Langerhans for transplantion of purified islets aiming to cure diabetes .

Human Islet Purification Miami 1998 • 20181102

Efficacy of the novel iodixanol-UWS density gradient for human islet purification

Slideshow (video) of my January 1999 oral presentation in Igls (Innsbruck), Austria, at the 18th Workshop of the AIDSPIT (Artificial Insulin Delivery Systems, Pancreas and Islet Transplantation) Study Group. (Now known as AIDPIT).

These results of this innovative islet purification method developed in our Leiden islet laboratory (LUMC) were the outcome of work I had done spring 1998 with the islet group of Camillo Ricordi in the “fast track” center for diabetes research at the Diabetes Research Institute in Miami , Florida, USA.

Notes

The slideshow (pdf) was first uploaded Nov. 11, 2010 at at Scribd , and Archive.org • URL http://archive.org/details/EfficacyOfTheNovelIodixanol-uwsDensityGradientForHumanIslet

Original video title (file name) : 20181102 ~ Human Islet Purification Miami 1998

Updates:
20241202 – Text updated with extra info. Youtube video in post replaced by Vimeo edition. Copied 1-memo post to michelvanderburg.com .

Citation info : Human Islet Purification Miami 1998 • Michel van der Burg • Miracles.Media • 20181102 • URL michelvanderburg.com/2018/11/02/ • TakeNode 3f2ec4d2-afe0-4580-b055-92a2f231a4fa

Out Of Service | 20140911

Out Of Service | 20140911 | 1-memo•com | In the morning of 9/11 2001 logistics forced me to officially announce a temporarily – 1 week – ‘out of service’ of our Leiden islet isolation and transplant center. Our center was started earlier that summer of 2001 at the Leiden University Medical Center. So, I decided to have a short vacation abroad – heaven, after being on call 24/7 almost non stop for years. Later that day at home, I was downstairs watching TV — because upstairs near my workroom the central heating was being replaced – when I saw the CNN breaking news about the plane crashing into the World Trade Center. That day would become a landmark in history and the course of my life.

Background image : Microscopy picture I took of purified human Islets of Langerhans – tiny clumps (size around 1/10 mm or 1/25 inch) of 10s to 1000s of cells that produce hormones like insulin – these cells are just visible when you enlarge this 50x microscopy picture : as tiny translucent ‘balls’ or ‘spheres’. The red color is from a stain that binds to the insulin cells.
Iodixanol-UWS purified human islets, stained with dithizone. Original magnification 50x.

Title: mvdb20041118_114555m0400.tif
Creator: Michael P.M. van der Burg
Caption/Description: Iodixanol-UWS purified human islets, stained with dithizone. Original magnification 50x. Copyright Notice: Copyright © 2004 M.P.M. van der Burg. All rights reserved. Digital Creation Date: 18 Nov 2004

References

Image published in : Transplantatie van eilandjes van Langerhans bij patiënten met diabetes mellitus type 1. Ned Tijdschr Geneeskd 2005 1 januari;149(1) – URL https://www.ntvg.nl/system/files/publications/2005100120001a.pdf

Efficacy of the novel iodixanol-UWS density gradient for human islet purification (Presentation publ. Archive Org – michelvanderburg, 19990125 )
https://archive.org/details/EfficacyOfTheNovelIodixanol-uwsDensityGradientForHumanIslet

25 Years of the Ricordi Automated Method for Islet Isolation. Piemonti L, Pileggi A. in CellR4 2013; 1(1): e128. – URL https://www.cellr4.org/article/128

Credit : Out Of Service | 20140911 | 1-memo•com | TakeNode ID e22f209c-14da-40d8-b11a-74a29b1e80b4

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