Pig Islet Isolation, Fondation Transplantation, Strasbourg 1992 • Michel van der Burg • Miracles.Media • @1MEMO 20250113_1 • TakeNode b1f66781-cf63-4e64-a7b3-24cbdf5c107f
For the xenotransplantation research we started in 1991 at our Leiden Surgery Department, I studied pig islet isolation during a working visit February 1992, at the ‘Fondation Transplantation’ in Strasbourg-Hautepierre, France — here watching our student Vincent Nieuwenhuijs (left) at work in the international team – with Agnaldo Soares I guess – using the Ricordi method for islet isolation — and presented the recent Leiden islet work (1).
Notes
1) Van der Burg MPM, Gooszen HG. Islet preservation using UW, and islet function after autotransplantation in dogs. Fondation Transplantation, CHU HautePierre Strasbourg (France) February 18, 1992.
Update 20250124 – Paper reference
Citation info : Xeno Islets • Michel van der Burg • Miracles.Media • @1MEMO 20250113 • URL michelvanderburg.com/2025/01/13/
No Title • Oil- Acrylic painting by Hans Dudart • Miracles.Media • @1MEMO 20250112_1 • TakeNode cbd20789-ebf5-4ede-925b-9b861c6719a4
Scan of print (10×15 cm; photo G.J. v.d. Poort) of oil- acrylic painting (60x50cm) by Hans Dudart.
Hans Dudart (1955-2003) worked as an artist in Holland, and as a valued co-worker in our Experimental Surgical Laboratory of the Leiden University Hospital (AZL, now LUMC) in Leiden, Holland. He died too soon from type 1 diabetes.
Citation info : No Title • Michel van der Burg • Miracles.Media • @1MEMO 20250112 • URL michelvanderburg.com/2025/01/12/
Islet Transplant Performance 5th ESOT 1991 • Michel van der Burg • Miracles.Media • @1MEMO 20250109_1 • TakeNode 75edbb4e-4360-43b8-8783-363ffd34c0ef
Poster (1) presented at the 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991. The poster was originally created on a Macintosh computer Oct 6, 1991, using the MacDraw II application, with graphs from Cricket Graph. That 1991 poster was remastered today in LibreOffice.
We presented 2 posters at the 5th ESOT, this poster (1), and another one on the effects of denervation with pancreas transplantation (2).
Two years after the 4th ESOT (in Barcelona, Nov 1989) where we introduced the UW-Solution as an islet isolation solution before density gradient purification, we now had a talk at this 5th ESOT on the use of the UW-Solution as the islet isolation solution, both before purification, and also during the purification in an innovative density gradient (3) — the foundation for current clinical purification methods. The talk was recorded on tape (except for the intro), and will be posted here soon too.
Notes
1) Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. Postprandial performance after canine islet transplantation: the importance of gut factors. 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991.
2) Guicherit OR, Van der Burg MPM, Lemkes HHPJ, Frölich M, Gooszen HG. Pancreatic denervation causes reduced insulin sensitivity. 5 th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) Oct 7–10 1991.
3) Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991.
Citation info : Islet Transplant Performance 5th ESOT 1991 • Michel van der Burg • Miracles.Media • @1MEMO 20250109 • URL michelvanderburg.com/2025/01/09/
Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202_1
I started an archive at Archive.org November 11, 2010 with this slideshow (pdf) of my January 1999 oral presentation in Igls (Innsbruck), Austria, at the 18th Workshop of the AIDSPIT (Artificial Insulin Delivery Systems, Pancreas and Islet Transplantation) Study Group. (Now known as AIDPIT).
The presented results of this innovative islet purification method — developed over nearly 10 years (from 1989) in our Leiden islet laboratory (LUMC) — were the outcome of work I had done spring 1998 with the human islet transplantation group of Camillo Ricordi at the Diabetes Research Institute in Miami , Florida, USA.
Slideshow (pdf) Archive.org
Description
Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999
Slides oral presentation Michel van der Burg, Aidspit meeting 25 Jan 1999, in Igls, Austria
Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification. 18th Workshop Aidspit, Igls (Austria) Jan. 24-26, 1999
Program : 18TH WORKSHOP OF THE AIDSPIT STUDY GROUP – JANUARY 24-26, 1999 – IGLS/AUSTRIA . MONDAY, JAN 25, 1999 – KONGRESSHAUS IGLS, MAIN LECTURE HALL SESSION 2 – ISLET ISOLATION AND TRANSPLANTATION CHAIRMEN:K. FEDERLIN, GIESSEN, GERMANY – G. POZZA, MILAN, ITALY 9.00 – 10.30 FREE COMMUNICATIONS 9.45- Efficacy of the novel iodixanol-UWS density gradient for human islet purification. Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C; Leiden, The Netherlands and Miami, USA
File : mvdb19990118_1534_AIDSPIT.pdf | Uploaded 20101111 and replaced by the same pdf with updated metadata of the document on 20101112 (see Notes section).
The slideshow video embedded below, was first posted in 2018 as Human Islet Purification Miami 1998 • 20181102
Notes
The slideshow pdf file is available for download at Archive.org. I advise against the other download options presented by Archive.org , which do not correctly represent the slideshow.
Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification (Abstract). Acta Diabetol 1998; 35: 247.
Citation info (this post) : Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202 • URL michelvanderburg.com/2024/12/02/