Successful Islet Transplantation After Purification in Iodixanol-UWS • IPITA 1999 Sydney • @1MEMO 20260326

Pig islets after isolation in Iodixanol-UWS • 7th IPITA World Congress 1999 • 1MEMO_20260326_1 • Michel van der Burg • Miracles•Media • TakeNode 467e773c-fc4b-416e-99d5-c88df76ddff4 • Presentation by Michel van der Burg (Notes #5).

Shortly after our introduction at the previous IPITA World Congress, September 1997 in Milan (Italy) of the novel Iodixanol -UWS solution for isolation of juvenile pig islets (1), we reported successful xeno-transplantation in diabetic Swiss nude (immunodeficient) mice of adult pig islets isolated with this Iodixanol -UWS method, first Tuesday, Jan 27, 1998 at the 17th Workshop of the AIDSPIT Study Group in Igls, Austria (2a), and next April 1998 at the 10th Congress of the Dutch Transplantation Society (2b). We had started these first pig islet xenotransplants in mice also that same month of September 1997 — highlighted in the image below ( 1MEMO_20260326_12 )

First pig islet xeno transplants in mice • 1MEMO_20260326_12 • Michel van der Burg • Miracles•Media • TakeNode d4cc1f11-c107-4011-8dbe-9649da76e3ff • September 1997, we performed our first two pig islet xenotransplants into diabetic Swiss nude mice. The pig islets were isolated with Iodixanol-UWS from an adult pig pancreas (top panel: sample of the islets stained red with dithizone, fresh after isolation and purification). These pure islets were kept 1 week in a culture solution, before transplantation of the islets by injecting the islets under the kidney capsule in 2 diabetic Swiss nude mice. Both mice had normal blood glucose from the day one after transplantation, and upon removal of the kidneys for examination at 1 month after transplantation , we observed excellent islets under the kidney capsule , as shown in the bottom panel image of a kidney section stained with H&E (note : the inserted portrait of the Swiss nude mouse, actually is our #7 pig islet xenograft, alive and kicking, Dec 1997).

Starting with the September 1997 IPITA meeting in Milan, I was sharing in detail the materials and methods of our purification procedure, face to face and in post-congress correspondence, with my colleagues of the other islets centers, thus facilitating fast implementation by Bernhard Hering and Shinichi Matsumoto of the Iodixanol-UWS purification method in the Minneapolis islet center, contributing to their successful single-donor pig islet allo-transplants, as reported by Shinichi Matsumoto May 1998 at the 24th Annual Scientific Meeting of the American Society of Transplant Surgeons, in Chicago (3).

That month, May 1998, I also finished the first series of human islet purifications with the novel Iodixanol-UWS density gradient, while working on human islet isolations in the Miami team at the Diabetes Research Institute (Scientific director Prof. C. Ricordi, MD) of the University of Miami , in Miami, Florida, USA . The successful outcome of the human islet purification work done in Miami was reported from January 1999 in a talk and poster presentations , including a poster presentation at the 7th World Congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 1999 (4).

Viability of Fresh vs Cultured Pig Islets for Transplants • 1MEMO_20260326_2

Slideshow presentation by Josephine Rijkelijkhuizen • 7th IPITA World Congress 1999 • Viability of Fresh vs Cultured Pig Islets for Transplants • 1MEMO_20260326_2 • Michel van der Burg • Miracles•Media • TakeNode 7164b724-65bf-4d3f-8a40-89b188957d1b • Notes #6

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Handout • PDF (Viability of Fresh vs Cultured Pig Islets for Transplants)
7th IPITA World Congress 1999 • 1MEMO_20260326_3 • Michel van der Burg • Miracles•Media • TakeNode 995e90b3-d797-435c-a510-00a0e35bc424 • Handout presentation by Josephine Rijkelijkhuizen (Notes #6).

In our oral presentations at the 7th World Congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 1999, we demonstrated both the complete recovery of pure intact pig islets during isolation in UWS and purification in the novel solution of Iodixanol (Optiprep) in UWS, and the superior viability of these islets following short-term storage in culture solution (‘culture’) , allowing successful xeno transplantation of the pig islets in mice (5,6), rats (7), and monkeys (8) .

The 7th IPITA congress abstracts were published online only at the official website of the IPITA, in Oxford at the time (www.jr2.ox.ac.uk/ipita), and cannot be retrieved now (9). However, I kept an archive with photocopies of our abstracts published in print in the Abstract Book of the 7th IPITA , posted below.

One of our papers was included among the top 10 papers selected for submission of a full paper in a special issue of the journal Cell Transplantation — see below.

Josephine Rijkelijkhuizen at the 7th IPITA World Congress 1999 • 1MEMO_20260326_4 • Photo by Michel van der Burg • Miracles•Media • TakeNode 0c2ef6fb-bdff-4895-9dde-eb242f957cb9

Top 10 Paper

Our study — collaborative research of our Surgery Department (LUMC), and workers of the Biomedical Primate Research Centre in Rijswijk (Netherlands) — presented by our student researcher Josephine Rijkelijkhuizen, showing that cultured pig islets, isolated and purified in Iodixanol-UWS, can be transplanted without hyperacute rejection in cynomolgous monkeys , was selected in the top 10 outstanding studies at the memorable Sidney World Congress of the International Pancreas & Islet Transplant Association (9) , and the full paper included in a special issue of the journal Cell Transplantation (10).

Our studies , together, demonstrated the importance of both donor organ selection (choosing adult organs), selection of non-toxic, endotoxin-free media, such as iodixanol and UWS, as the isolation solutions, and selection of islets that survive storage in culture, for a successful outcome after transplantation in this difficult model of xeno-transplantation, grafting porcine islets in other species.

Notes

1) Innovative Islet Purification in Iodixanol-UWS • IPITA 97 • Michel van der Burg • Miracles.Media • @1MEMO 20250128 • URL michelvanderburg.com/2025/01/28/

2a) Iodixanol-UWS Purification for Porcine Islet Transplantation • AIDSPIT 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250131 • URL michelvanderburg.com/2025/01/31/

2b) Fresh vs Cultured Pig Islets for Xenografts • NTV Bootcongres 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250201 • URL michelvanderburg.com/2025/02/01/

3) Matsumoto S, Zhang H J, Gilmore T, van der Burg MPM, Sutherland DER, Hering BJ. Large scale isopycnic islet purification utilizing non-toxic, endotoxin-free media facilitate immediate single-donor pig islet allograft function (Abstract). Transplantation 1998; 66: S30 • 1MEMO_20260326_10 • Michel van der Burg • Miracles.Media • TakeNode a7e8ec21-1757-45f4-90c6-1a5f457e0d9f , View/download PDF below.

1MEMO_20260326_10 Download

Presentation at the 24th Annual Scientific Meeting of the American Society of Transplant Surgeons, Chicago (USA) May 1998. 24th ASTS Program book, Abstract #A-120 • 1MEMO_20260326_5 • Michel van der Burg • Miracles.Media • TakeNode 9efdd5cb-f6da-4dec-b9d9-63cf0b254b30

4) Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Terpstra OT, Ricordi C. New powerful tool for human islet purification: OptiPrep-UWS? 7th World Congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999. Abstract in Programme and Abstract Book, page 169, P298 . In : New Powerful Tool For Human Islet Purification • OptiPrep-UWS ? • IPITA 1999 Sydney • @1MEMO 20260325 • Michel van der Burg • Miracles.Media • URL michelvanderburg.com/2026/03/25/

5) Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Recovery of adult pig islets during isolation using Liberase vs collagenase-P, purification in OptiPrep-UWS, and culture for transplantation in nude mice. 7th World congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999. Programme and Abstract Book, page 164, #281 . 1MEMO_20241203_3 • In : Iodixanol Purification of Porcine Islets • Sydney IPITA 1999 • 1MEMO 20241203 • Michel van der Burg • Miracles.Media • TakeNode 64ace253-d25b-4b6d-af5b-08060712c471 • URL michelvanderburg.com/2024/12/03/

6) Rijkelijkhuizen JKRA, Bouwman E, Van der Burg MPM. Pre-transplant culture markedly improves pig islet graft survival in nude mice. 7th World congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999. Programme and Abstract Book, page 117, #162 . 1MEMO_20260326_6 • Michel van der Burg • Miracles.Media • TakeNode 5ce0c54f-a6a1-4cc9-8f68-9a1cd723badf

7) Rijkelijkhuizen JKRA, Van der Burg MPM, Bouwman E. Longterm survival of porcine islets in the rat. 7th World congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999. Programme and Abstract Book, page 112, #152 . 1MEMO_20260326_7 • Michel van der Burg • Miracles.Media • TakeNode bc9a48ee-23ff-48f1-ab2c-5bcef071ebaa

8) Rijkelijkhuizen JKRA, Bouwman E, Van der Burg MPM, Ringers J, Ossevoort MA, Kuhn EM, Frost P, Jonker M. Successful suppression of the early rejection of pig islets in monkeys. 7th World congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999. Programme and Abstract Book, page 144, #231 . 1MEMO_20260326_8 • Michel van der Burg • Miracles.Media • TakeNode 29bb03b4-8b02-4aa9-9b33-715ca006c28e

9) Ricordi C. Prime Time for Pancreas and Islet Transplantation at the Turn of the Millennium. Cell Transplant. 2000 Nov;9(6):853-855. doi: 10.1177/096368970000900611. PMID: 28886292. URL https://journals.sagepub.com/doi/epdf/10.1177/096368970000900611 (Free Full Paper)

10) Rijkelijkhuizen JK, Bouwman E, van der Burg MP, Ringers J, Ossevoort MA, Kuhn EM, Frost P, Jonker M. Successful suppression of the early rejection of pig islets in monkeys. Cell Transplant. 2000 Nov-Dec;9(6):909-12. doi: 10.1177/096368970000900618. PMID: 11202577. URL https://journals.sagepub.com/doi/epdf/10.1177/096368970000900618 (Free Full Paper) • Collaborative research From Biomedical Primate Research Centre (M.O., E.M.K.,
J.W., K.H., M.J.), Rijswijk, and Leiden University Medical Centre (J.K.R.A.R., J.R., M.vdB., E.B.), Leiden, The Netherlands .

Sydney Convention Center • 1MEMO_20260326_9 • Michel van der Burg • Miracles.Media • TakeNode 1ce6bbf2-adf1-4ce3-8c69-cd2d1339be81 • Sydney Convention Center – Darling Harbour (demolished in 2013)

Cultured pig islets for xenotransplantation • 1MEMO_20260326_11 • Michel van der Burg • Miracles•Media • TakeNode bd15f087-29cd-4e99-a555-9fbfbd1729bc • Reflection contrast microscopy (ultrathin section) of 1-week cultured pig islets . During storage in culture remodeling of the isolated islets occurs, showing rounded islets , with some central necrosis after prolonged culture. Transplantation of 1250 islets (IEQs) from this same 1-week cultured pig islet preparation cured all (n=3) diabetic nude mice indefinitely (over 150 days). In contrast transplants of freshly isolated islets (not cultured) using the same dose of 1250 islets (IEQs) from this same islet preparation, did not cure diabetic nude mice (n=2). Adult pig pancreas procurement by Michel van der Burg from the nearby Westgeest slaughterhouse (in Zoetermeer) facility for large sows processing by Ballering Export (Son, Netherlands; a company of second cousins, of mine).
. Islet isolation (using Iodixanol-UWS) and transplants team work by Josephine Rijkelijkhuizen and Michel van der Burg, Leiden Islet Laboratory (LUMC), May 1998. Reflection contrast microscopy by Frans A. Prins and Michel van der Burg. Not previously published data and photo by Michel van der Burg.

#IPITA #Sydney #surgery #DRI #Miami #clinical #laboratory #purification #optiprep #iodixanol #density #gradient #UWS #pancreas #human #pig #rat #mouse #monkey #xeno #islets #islet #Langerhans #isolation #UW #university #Wisconsin #transplantation #diabetes #man #history #Holland #Netherlands #Leiden #hospital #LUMC #1memo #michelvanderburg #Rijkelijkhuizen #Matsumoto #Hering #Minneapolis #Ricordi

Citation info : Successful Islet Transplantation After Purification in Iodixanol-UWS • IPITA 1999 Sydney • @1MEMO 20260326 • Michel van der Burg • Miracles.Media • URL michelvanderburg.com/2026/03/26/

New Powerful Tool For Human Islet Purification • OptiPrep-UWS ? • IPITA 1999 Sydney • @1MEMO 20260325

7th IPITA World Congress 1999 • 1MEMO_20260325_1 • Michel van der Burg • Miracles•Media • TakeNode 770715b8-ca68-4c00-8a24-24fe0d216c10

Poster presentation (#P298) Michel van der Burg, 7th World Congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999 (1).

Outcome of the first series of human islet purifications with the novel Iodixanol -UWS density gradient — developed originally in the difficult model of pig islet isolation in our Leiden Islet Laboratory of the LUMC (2) — and, performed in 1998 during my working visit at the “fast track” center of the Diabetes Research Institute (Scientific director Prof. C. Ricordi, MD) of the University of Miami , in Miami, Florida, USA .

Notes

1) Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Terpstra OT, Ricordi C. New powerful tool for human islet purification: OptiPrep-UWS? 7th World Congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999. Abstract in Programme and Abstract Book, page 169, P298 .

Abstracts of previous IPITA meetings had been published in print in scientific journals (2), however, at the turn of the millennium in 1999, with the rise of the internet , the 7th IPITA congress abstracts were published online only at the official website of the IPITA, in Oxford at the time (www.jr2.ox.ac.uk/ipita), and cannot be retrieved now (3).
I kept an archive with photocopies of our abstracts published in print in the Abstract Book of the 7th IPITA. Below that abstract of poster presentation P298 at page 169 in the Programme and Abstract Book.

7th IPITA World Congress 1999 • 1MEMO_20260325_2 • Michel van der Burg • Miracles•Media • TakeNode 9a8affeb-120a-4484-a390-fe058ac64af4

A previous poster on this human islet work had been presented at the 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999. See:
Optiprep For Human Islet Purification • Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20250203 • URL michelvanderburg.com/2025/02/03/

A previous talk on this human islet work had been presented Monday, 25 January 1999, at the 18th Workshop of the AIDSPIT Study Group in Igls , Austria. See :
Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202 • URL michelvanderburg.com/2024/12/02/

2) Innovative Islet Purification in Iodixanol-UWS • IPITA 97 • Michel van der Burg • Miracles.Media • @1MEMO 20250128 • TakeNode • URL michelvanderburg.com/2025/01/28/

3) Ricordi C. Prime Time for Pancreas and Islet Transplantation at the Turn of the Millennium. Cell Transplant. 2000 Nov;9(6):853-855. doi: 10.1177/096368970000900611. PMID: 28886292. URL https://journals.sagepub.com/doi/epdf/10.1177/096368970000900611

Citation info : New Powerful Tool For Human Islet Purification • OptiPrep-UWS ? • IPITA 1999 Sydney • @1MEMO 20260325 • Michel van der Burg • Miracles.Media • URL michelvanderburg.com/2026/03/25/

Assessment of isolated islet equivalents • Cell Transplant Society 1996 • @1MEMO 20251205

Assessment of isolated islet equivalents • Cell Transplant Society 1996 • Michel van der Burg • Miracles.Media • 1MEMO_20251205_1 • Poster 3rd International Congress Cell Transplant Society, Miami (USA) Sep 29 – Oct 2, 1996 • TakeNode 1300e7cd-85a4-4cd0-9cbc-a88746e625ab • #IEQ #diabetes #islets #transplant #methods #CTRMS

IEQ – Islet Equivalent

IEQ islet stands for Islet Equivalent, a standard unit used to measure the total volume of islets (clusters of insulin cells and other hormone-producing cells in the pancreas). It is defined as a single spherical islet with a diameter of 150 µm. The IEQ is a crucial parameter for assessing the quantity of islets for procedures like clinical islet transplantation, which is a treatment for type 1 diabetes.

Modified international procedure

Following a consensus report in 1989 – 1990 by Ricordi et al (1a, 1b) on islet isolation assessment, the total volume of isolated islets is generally expressed in the number of islet equivalents (IEQs) — defined as islets of 150 µm diameter— and many centers use the advocated now classic international procedure (CIP) of sizing all islets in classes with 50-µm increments (i.e., 50–100, 100–150, etc.) for calculation of the number of IEQs. For each class the number of IEQs are calculated by multiplying the islet counts with a conversion factor — based on the mean volume of that class.

A modified international procedure — based on our conventional Leiden procedure (2,3) with islet sizing in classes with 25-µm increments — is suggested for accurate assessment of the IEQs especially when isolation yields mostly smaller islets, in our paper ‘Assessment of isolated islet equivalents’ presented at the 3rd International Congress Cell Transplant Society, Miami (USA) Sep 29 – Oct 2, 1996, in both a poster (4) , and proceedings paper (5). A photo of the original poster , (retrieved somewhat wrinkled 30 years later from my archive), is posted here, as well as a pdf from the (corrected proof) manuscript of the proceedings paper below (6).

The Cell Transplant Society was founded in 1991, and has been renamed the Cell Transplant and Regenerative Medicine Society (CTRMS).

Notes

1a. Ricordi C, Gray DW, Hering BJ, Kaufman DB, Warnock GL, Kneteman NM, Lake SP, London NJ, Socci C, Alejandro R, et al. Islet isolation assessment in man and large animals. Acta Diabetol Lat. 1990 Jul-Sep;27(3):185-95. doi: 10.1007/BF02581331. PMID: 2075782.

1b. Quantitative And Qualitative Standards For Islet Isolation Assessment In Man And Large Mammals. International Workshop during the 2nd Congress on Pancreas and Islet Transplantation (1989). Camillo Ricordi. Archive Michel van der Burg • Miracles•Media • 1MEMO_20251206_07 . Available from Dec 6, 2025 URL https://michelvanderburg.com/2025/12/06/

2. Van der Burg MPM, Guicherit OR, Frölich M, Scherft JP, Prins FA, Bruijn JA, Gooszen HG. Assessment of islet isolation efficacy in dogs. Cell Transplant. 1994 Jan-Feb;3(1):91-101. doi: 10.1177/096368979400300113. PMID: 8162296.

3. Paper (Notes 2) included as Chapter 3 in my doctoral thesis (Leiden University, November 1994) : Assessment of islet isolation efficacy in dogs. In : Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation ( Chapter 3). (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Nov 24th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 . Direct link to file Chapter 3 , URL https://scholarlypublications.universiteitleiden.nl/access/item%3A3486610/view

4. Van der Burg MPM, Scheringa M, Basir I, Bouwman E. Assessment of isolated islet equivalents. Poster presented at the Third Int. Congress Cell Transplant Soc., Miami (USA) Sept. 29 – Oct. 2, 1996.
In : Assessment of isolated islet equivalents • Cell Transplant Society 1996 • Michel van der Burg • Miracles.Media • @1MEMO 20251205 • URL https://michelvanderburg.com/2025/12/05 • File 1MEMO_20251205_1 • TakeNode 1300e7cd-85a4-4cd0-9cbc-a88746e625ab

5. Van der Burg MPM, Scheringa M, Basir I, Bouwman E. Assessment of isolated islet equivalents. Transplant Proc. 1997 Jun;29(4):1971-1973. doi: 10.1016/s0041-1345(97)00188-7. PMID: 9193483.

6. Manuscript (corrected proof for Transplant Proc paper PMID: 9193483). Van der Burg MPM, Scheringa M, Basir I, Bouwman E. Assessment of isolated islet equivalents • 1MEMO_20251205_2 • TakeNode 48380f3c-b3f7-416e-90bb-ad6c53129e46 • PDF available below , File 1MEMO_20251205_2

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#IEQ #diabetes #islets #transplant #methods #CTRMS

Citation info : Assessment of isolated islet equivalents • Cell Transplant Society 1996 • Michel van der Burg • Miracles.Media • @1MEMO 20251205 • TakeNode 1300e7cd-85a4-4cd0-9cbc-a88746e625ab / 48380f3c-b3f7-416e-90bb-ad6c53129e46 • URL https://michelvanderburg.com/2025/12/05

Glycemic control mechanisms after canine islet autografting • IPITA 1995• @1MEMO 20251204

Glycemic control mechanisms after canine islet autografting • IPITA 1995 • Michel van der Burg • Miracles.Media • @1MEMO 20251204_1 • Talk, Tuesday June 20, 1995 at 10:30, 5th IPITA, Eden Roc Resort Hotel, Miami Beach, FL (USA) • Digitally remastered by author, publisher, Michel van der Burg • TakeNode 5227c200-e029-4406-b344-15350fe3b93d • #GLP1 #GIP #diabetes #islets #transplant

Pioneer work on GLP-1 and GIP incretin hormones

Slideshow (digitally remastered) of my talk Tuesday June 20, 1995 at 10:30, at the 5th International Congress on Pancreas and Islet Transplantation (IPITA), in Miami Beach, FL (USA). My first talk at a world congress presenting the outcome of all our detailed metabolic studies in our preclinical model of canine islet autotransplantation , including our pioneer work on the incretin effects of the gut hormones GLP-1 (glucagon-like peptide-1) and GIP (Glucose-dependent Insulinotropic Polypeptide) — now, in 2025, well-known from the drugs that mimic the action of these natural hormones, to treat type 2 diabetes and obesity, by stimulating insulin release, improving blood sugar control, and reducing appetite.

May 1992 we were the first to show direct potentiation of the insulin response from isolated islets by (near-)physiological doses of both the gut hormones GLP-1 and GIP during perifusion (in vitro) of canine islets at 7.5-10 mM glucose levels (1,2), and January 1994 we next confirmed the incretin effect of a (near-)physiological dose of GLP-1 after canine islet transplantation , in a poster presented at the 13th Workshop of the AIDSPIT (aka AIDPIT) Study Group , Igls, Austria, January 23–25, 1994 (3).

Background of the work was posted earlier this year (4) and the work was published Nov 1994 in my PhD thesis Pancreatic islet transplantation , available online (5).

Welcome Reception 5th IPITA on Sunday 18 June 1995, at the congress venue , the Eden Roc Resort Hotel, in Miami Beach, FL (USA) • Michel van der Burg • Miracles.Media • @1MEMO 20251204_2 • TakeNode 8eb04df1-8280-4de3-a206-4b63413c3025

June 1995, 5th IPITA Talk

Tuesday June 20, 1995 at 10:30, at the 5th International Congress on Pancreas and Islet Transplantation (IPITA), in Miami Beach, FL (USA), was my first talk at a world congress presenting the outcome of all our detailed metabolic studies in the canine islet transplants , including the incretin effect of GLP-1, and also our findings with isolated canine islets during perifusion, in vitro, of the direct insulinotropic effects of the gut hormones GIP and GLP-1 (6) . That slideshow presentation (digitally remastered*) is posted here (1MEMO 20251204_1).

The proceedings paper delivered at the 5th IPITA is included in the Notes (7) , as well as the referenced previous papers (8,9)

Notes

1. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Terpstra JL, Gooszen HG. Functie na intralienale transplantatie van eilandjes van Langerhans in de hond ( Function after intrasplenic transplantation of islets of Langerhans in dogs ). Surgeon days ’92 of the Dutch Surgery Society, Rotterdam (The Netherlands) May 21–22, 1992. In : Gut factors controlling pancreatic islets • 20240910 • Michel van der Burg • michelvanderburg•com • URL https://michelvanderburg.com/2024/09/10/gut-factors-controlling-pancreatic-islets-20240910/

2. Van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Pept. 1995 Dec 7;60(1):61-7. doi: 10.1016/0167-0115(95)00122-4. PMID: 8747785.

3. Metabolic control after canine islet transplantation. M.P.M. van der Burg, P.T.R. van Suylichem, O.R. Guicherit, J.H.M. van Deijnen, M. Frölich, and H.G. Gooszen (Universities of Leiden & Groningen, The Netherlands). Poster presented at the 13th Workshop of the AIDSPIT Study Group , Igls, Austria, January 23–25, 1994 • In : GLP-1 blood sugar regulation in islet transplants • AIDSPIT 1994 • Michel van der Burg • Miracles.Media • @1MEMO 20251114 • TakeNode 810cc881-d084-4d2e-b252-d432918b1946 • URL https://michelvanderburg.com/2025/11/14

4. Background of this work was posted in Dutch Islets Transplant Team • @1MEMO 20250125 • URL https://michelvanderburg.com/2025/01/25/dutch-islets-transplant-team-1memo-20250125/

5. Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation. (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Dec 4th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 .

6. Oral presentation : Van der Burg MPM, Van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glycemic control mechanisms after canine islet autografting. 5th International Congress on Pancreas and Islet Transplantation (IPITA), Miami Beach (USA) June 20, 1995.

7. 5th IPITA Proceedings publication : Van der Burg MPM, Van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glycemic control mechanisms after canine islet autografting. Transplant Proc. 1995 Dec;27(6):3187-8. PMID: 8539903. PDF available below PMID_8539903

PMID_8539903 Download

Referenced previous publications :

8. Van der Burg MPM, Gooszen HG. The metabolic efficiency of islet grafts: an overview. In: Islet transplantation — current status of clinical application and experimental results. Hesse UJ , Pichlmaier H, Eds., Lengerich Germany, Wolfgang Pabst Verlag, 1992, p.93–99. Pabst hash code 0383b3abad3ae774b146ea0425c2227a PDF available below 1MEMO_20251204_4

1MEMO_20251204_4 Download

9. Van der Burg MPM, Guicherit OR, Scherft JP, Lemkes HHPJ, Frölich M, Gooszen HG. Postprandial performance after canine islet transplantation: the importance of gut factors (Abstract). Neth J Med 1992; 41: A25. PDF available below 1MEMO_20251204_3

1MEMO_20251204_3 Download

Other previous publications :

10. Van der Burg MPM, Guicherit OR, Van Suylichem PTR, Frölich M, Van Deijnen JHM, Gooszen HG. Importance of the entero-insular axis for islet graft function (Abstract). Cell Transplant 1994; 3: 257. Abstract – Second International Congress of the Cell Transplant Society May 1–4, 1994 Hyatt Regency Hotel • Minneapolis, Minnesota . https://doi.org/10.1177/096368979400300302 (Abstracts published , posters not presented). PDF available below 10.1177_096368979400300302

10.1177_096368979400300302 Download

11. Chapter 8, Doctoral Thesis, Leiden University, November 1994 : Metabolic control after intrasplenic islet autotransplantation in dogs: ß-cell secretory capacity, insulin action, and the enteroinsular axis. Michael P.M. van der Burg, Paul T.R. van Suylichem, Onna R. Guicherit, Marijke Frölich, Herman H.P.J. Lemkes, and Hein G. Gooszen. In : Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation ( Chapter 8). (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Nov 24th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 .
Direct link to file Chapter 8 , URL https://scholarlypublications.universiteitleiden.nl/access/item%3A3486615/view .

12. Doctoral Thesis, Leiden University, November 1994 (Chapter 6) : lnsulinotropic properties of cholecystokinin, gastric inhibitory polypeptide, and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Michael P.M. van der Burg, Onna R. Guicherit, Marijke Frölich and Hein G. Gooszen. In : Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation ( Chapter 6). (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Nov 24th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 .
Direct link to file Chapter 6 , URL https://scholarlypublications.universiteitleiden.nl/access/item%3A3486613/view

Full papers

13. Van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Pept. 1995 Dec 7;60(1):61-7. doi: 10.1016/0167-0115(95)00122-4. PMID: 8747785.

14. Van der Burg MPM, Van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glucoregulation after canine islet transplantation: contribution of insulin secretory capacity, insulin action, and the entero-insular axis. Cell Transplant. 1997 Sep-Oct;6(5):497-503. doi: 10.1177/096368979700600509. PMID: 9331501. PDF link https://pure.rug.nl/ws/portalfiles/portal/58359942/096368979700600509.pdf

* 1MEMO 20251204_1 . Digitally remastered presentation, using the images from the original slides handout, and the handout of the text spoken during the original presentation , now read using my personal synthesized voice.

#GLP1 #GIP #diabetes #islets #transplant

Citation info : Glycemic control mechanisms after canine islet autografting • IPITA 1995 • Michel van der Burg • Miracles.Media • @1MEMO 20251204 • URL https://michelvanderburg.com/2025/12/04

Iodixanol-UWS Human Islet Purification 1998 Abstract • @1MEMO 20251102

Front page Acta Diabetologica • Download Abstract Below

Abstract published in Acta Diabetologica 1998 of Iodixanol-UWS Human Islet Purification in 1998 at the Diabetes Research Institute in Miami. Abstracts book of the 18th Workshop of the AIDSPIT Study Group in Igls, Austria, Jan 1999.

Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification (Abstract). Acta Diabetol 1998; 35: 247.
View / Download abstract (file 1MEMO_20251102_1.pdf) below .

1MEMO_20251102_1 Download

Notes

Abstract added in full post :
Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • 20241202 • URL michelvanderburg.com/2024/12/02 •

Citation info : Iodixanol-UWS Human Islet Purification 1998 Abstract • Michel van der Burg • Miracles.Media • @1MEMO 20251102 • URL michelvanderburg.com/2025/11/02/

Human Islet Isolation • DRI Miami 1998 • @1MEMO 20250204

Summer 1997, Onno Terpstra, the head of our Surgery Department, asked me to explore our options for starting clinical islet isolation and transplantation in our center – the Leiden University Medical Center (LUMC), and prepare a roadmap.

September 1997, at the IPITA congress in Milan (1) , I learned from Camillo Ricordi, the scientific director of the Diabetes Research Institute (DRI) in Miami – where many guest collaborators work – that visiting the DRI some weeks might be a good option to gain sufficient first work experience in his clinical islet transplantation group.

December 1997, with a ‘Roadmap’, I got the green light to start the operationalization of clinical islet transplantation in the LUMC, first with a working visit to Miami to study the state of the art ‘Ricordi’ method of human islet isolation, and then start the human islet isolation work in our Surgery lab in Leiden.

In 1990 I had already gained some initial research experience in our Leiden Islet Laboratory with a small number of isolations of islets from human donor organs, using our innovative method of density gradient purification based on the UW solution (2).

During the first three months of 1998 I worked intensively with my student colleague Josephine Rijkelijkhuizen so that she would be able to continue our xenotransplantation research project as independently as possible in our Surgery research team.

April 1998, after participating in the 1st Workshop on Clinical Islet Transplantation in New Orleans (3) – the ideal introduction – a fruitful working visit to the DRI in Miami followed (4,5), with intensive collaboration with the DRI team on, among other things, six human islet isolations, and also the simultaneously (parallel) conducted research into the purification of the human islets in our Iodixanol gradient in UW-Solution (6).

Here a slideshow illustrating this team up with the DRI team in Miami , working on human islet isolation and purification in the innovative Iodixanol-UWS gradient, April-May 1998.

Notes

1) Innovative Islet Purification in Iodixanol-UWS • IPITA 97 • Michel van der Burg • Miracles.Media • @1MEMO 20250128 • TakeNode • URL michelvanderburg.com/2025/01/28/

2) Percoll UWS Purification Pancreatic Islets • ESOT 1991 Talk • Michel van der Burg • Miracles.Media • @1MEMO 20250114 • URL michelvanderburg.com/2025/01/14/

3) Invited participant 1st Workshop on Clinical Islet Transplantation, New Orleans (LA, USA) April 17–19, 1998

4) Working visit April – May 1998 at the “fast track” center of the Diabetes Research Institute (Scientific director Prof. C. Ricordi, MD) of the University of Miami (Miami, FL, USA)

5) DRI Dream Team • Michel van der Burg • Miracles.Media • @1MEMO 20250105 • URL michelvanderburg.com/2025/01/05/

6) Human Islet Purification Miami 1998 • Michel van der Burg • Miracles.Media • 20181102 • URL michelvanderburg.com/2018/11/02/

Citation info : Human Islet Isolation • DRI Miami 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250204 • TakeNode 86e4a319-a769-44d5-8fa5-de2ca90ab63c • URL michelvanderburg.com/2025/02/04/

Optiprep For Human Islet Purification • Cell Transplant Society 1999 • @1MEMO 20250203

Optiprep For Human Islet Purification • Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20250203 • TakeNode bad757b8-674c-4371-938c-ac73287bc457

Poster (#57) presentation Michel van der Burg, Cell Transplant Society congress 1999 in Montreux, Switzerland (1,2)

Outcome of the first series of human islet purifications with the novel Iodixanol -UWS density gradient — developed in the Leiden Islet Laboratory of the LUMC — performed during my working visit April – May 1998 at the “fast track” center of the Diabetes Research Institute (Scientific director Prof. C. Ricordi, MD) of the University of Miami (Miami, FL, USA).

Notes

1) Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Terpstra OT, Ricordi C. Optiprep for human islet purification. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999 .

2) Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Terpstra OT, Ricordi C. Optiprep for human islet purification (Abstract). Cell Transplant 1999; 8: 184. Download abstract below 1MEMO_20250202_3🔗
https://michelvanderburg.com/wp-content/uploads/2025/02/1memo_20250202_3.pdf

Citation info : Optiprep For Human Islet Purification • Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20250203 • URL michelvanderburg.com/2025/02/03/