Tag: porcine

Iodixanol-UWS Purification and Culture of Pig Islets Xeno Transplants • Cell Transplant Society 1999 • @1MEMO 20250202


Iodixanol-UWS Purification and Culture of Pig Islets Xeno Transplants • Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20250202

Slideshow (digitally remastered) oral presentation Michel van der Burg, Cell Transplant Society congress 1999 in Montreux, Switzerland (1)

The Leiden Islet Laboratory developed a novel Iodixanol-UWS density gradient for purification of the islets of Langerhans from the pancreas in the difficult pig model.

Pig islet isolation from the pancreas of slaughterhouse sows was markedly improved by keeping the islets in the UW donor organ preservation solution (UWS) both during the isolation of islets (using the Liberase enzyme blend) and purification of the islets using our novel Iodixanol-UWS density gradient.

Xeno transplantation of these 1-day-cultured pig islets restored normal blood sugar levels in diabetic mice.

PDF slideshow oral presentation Michel van der Burg, Cell Transplant Society congress 1999 in Montreux, Switzerland

Iodixanol-UWS Purification and Culture of Pig Islets Xeno Transplants • Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20250202_2 • TakeNode 8bd79256-78b2-4d8e-8d48-6da41102dc2d • Download file available below 1MEMO_20250202_2

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Notes

1) Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Adult pig islet recovery during Liberase isolation, OptiPrep purification and culture for transplantation in nude mice. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999 .

2) Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Adult pig islet recovery during Liberase isolation, OptiPrep purification and culture for transplantation in nude mice (Abstract). Cell Transplant 1999; 8: 185. Download abstract, available below 1MEMO_20250202_3

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Citation info : Iodixanol-UWS Purification and Culture of Pig Islets Xeno Transplants • Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20250202 • TakeNode f9d1fdc5-21e4-4932-b995-4c97639945fe • URL michelvanderburg.com/2025/02/02/

Purification Islets in Iodixanol-UWS • Leiden Start 1996 • @1MEMO 20250126

Purification Islets in Iodixanol-UWS • Leiden Start 1996 • Michel van der Burg • Miracles.Media • @1MEMO 20250126_1 • TakeNode c2a26daf-2c4f-4051-931d-16babc872c82

August 1995, I started designing the clinical promising islet purification solution of Iodixanal in UW-Solution (UWS). I mixed Optiprep – a 60 % solution of iodixanol in water – with a 2-times concentrated UWS, to prepare this density gradient solution. The first standard operation procedure of this Iodixanol-UWS gradient for our pig islet isolation research was completed November 1995.


This purification solution was a logical follow-up of our highly succesful Percoll-UWS density gradient — a similar solution of impermeants (that do not readily enter cells) with similar osmolarity (close to that of the tissue) , more practical though, and with the important advantage that iodixanol is clinical tested.
The Leiden Islet Lab had just moved into the Surgery Lab of the new LUMC building, and the islet team had grown, focussing on xenotransplantation. With more people isolating pig islets, more convenient solutions were tried.

Both Percoll-UWS and and the more convenient Histopaque-UWS were being used from 1992 for small scale islet isolations from juvenile pig pancreases.

July 1996, I introduced the Optiprep-UWS gradient to replace our Percoll-UWS gradient for larger scale porcine islet isolations , in comparison with the Histopaque gradient.

This work was first presented September 1997, both in this poster presented at the Xenotransplantation Congress in Nantes (France) and later that month also in a talk at the 6th Congress of the International Pancreas and Islet Transplant Association (IPITA) in Milan (Italy) — presentations that will be online at this site soon.

Notes

Van der Burg MPM, Basir I, Bouwman E. Non-traumatic porcine islet density gradient purification in a novel iodixanol in University of Wisconsin solution. The 4th Int. Congress for Xenotransplantation, Nantes (France) Sept. 7–11, 1997.

Update 20250128 – update intro design timeline

Citation info : Purification Islets in Iodixanol-UWS • Leiden Start 1996 • Michel van der Burg • Miracles.Media • @1MEMO 20250126 • URL michelvanderburg.com/2025/01/26/

Viability of Fresh vs Cultured Pig Islets for Transplants • Cell Transplant Society 1999 Montreux • 20241204


Oral presentation Michel van der Burg, Cell Transplant Society congress 1999 in Montreux, Switzerland

A novel iodixanol density gradient for purification of pancreatic islets was developed in the difficult pig model of islet isolation. Viability of freshly isolated islets is not guaranteed.
After short-term storage of the islets in culture, pig islets restored normal blood sugar levels after xeno transplantation in diabetic mice.


1-Day Cultered Islets (extra slide) • @1MEMO 20241204_1

Slideshow (pdf)

Viability of Fresh vs Cultured Pig Islets for Transplants • Montreux Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20241204_2 • TakeNode e745d625-27c2-4eb8-b4c2-4300623c3303 • File 1MEMO_20241204_2

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Rijkelijkhuizen JKRA, Bouwman E, Van der Burg MPM. Viability of fresh vs cultured pig islets for transplants. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999

Other Presentations in Montreux

Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Adult pig islet recovery during Liberase isolation, OptiPrep purification and culture for transplantation in nude mice. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999 . The Sydney 1999 presentation of this work has been posted – @1MEMO 20241203

Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Terpstra OT, Ricordi C. Optiprep for human islet purification. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999 . The Igls 1999 presentation of this work has been posted – @1MEMO 20241202

Citation info : Viability of Fresh vs Cultured Pig Islets for Transplants • Cell Transplant Society 1999 Montreux • Michel van der Burg • Miracles.Media • @1MEMO 20241204 • TakeNode 3ba1c894-eb71-4c3f-89aa-198c48cd480c • URL michelvanderburg.com/2024/12/04/

Iodixanol Purification of Porcine Islets • Sydney IPITA 1999 • 20241203

Slides oral presentation Michel van der Burg, IPITA congress 1999 in Sydney, Australia

A novel iodixanol density gradient for purification of pancreatic islets was developed in the difficult pig model. The pig islets restored normal blood sugar levels after xeno transplantation in diabetic mice.

Slideshow (pdf) oral presentation Michel van der Burg, IPITA congress 1999 in Sydney, Australia

Recovery of adult pig islets during isolation using Liberase vs collagenase-P, purification in OptiPrep-UWS, and culture for transplantation in nude mice • Sydney IPITA 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20241203_2 • TakeNode 5f53ae49-6fb8-4bef-a434-ac2ce8151c2a • File 1MEMO_20241203_2

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Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Recovery of adult pig
islets during isolation using Liberase vs collagenase-P, purification in OptiPrep-UWS, and
culture for transplantation in nude mice. 7th World congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999

Citation info : Iodixanol Purification of Porcine Islets • Sydney IPITA 1999 • Michel van der Burg • Miracles.Media • 1MEMO 20241203 • TakeNode ea1f1abd-6227-4ae6-985b-f8188310cd24 • URL michelvanderburg.com/2024/12/03/