Tag: purification

Viability of Fresh vs Cultured Pig Islets for Transplants • Cell Transplant Society 1999 Montreux • 20241204


Oral presentation Michel van der Burg, Cell Transplant Society congress 1999 in Montreux, Switzerland

A novel iodixanol density gradient for purification of pancreatic islets was developed in the difficult pig model of islet isolation. Viability of freshly isolated islets is not guaranteed.
After short-term storage of the islets in culture, pig islets restored normal blood sugar levels after xeno transplantation in diabetic mice.


1-Day Cultered Islets (extra slide) • @1MEMO 20241204_1

Slideshow (pdf)

Viability of Fresh vs Cultured Pig Islets for Transplants • Montreux Cell Transplant Society 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20241204_2 • TakeNode e745d625-27c2-4eb8-b4c2-4300623c3303 • File 1MEMO_20241204_2

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Rijkelijkhuizen JKRA, Bouwman E, Van der Burg MPM. Viability of fresh vs cultured pig islets for transplants. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999

Other Presentations in Montreux

Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Adult pig islet recovery during Liberase isolation, OptiPrep purification and culture for transplantation in nude mice. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999 . The Sydney 1999 presentation of this work has been posted – @1MEMO 20241203

Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Terpstra OT, Ricordi C. Optiprep for human islet purification. 4th International congress of the Cell Transplant Society, Montreux (Switzerland) March 21–24, 1999 . The Igls 1999 presentation of this work has been posted – @1MEMO 20241202

Citation info : Viability of Fresh vs Cultured Pig Islets for Transplants • Cell Transplant Society 1999 Montreux • Michel van der Burg • Miracles.Media • @1MEMO 20241204 • TakeNode 3ba1c894-eb71-4c3f-89aa-198c48cd480c • URL michelvanderburg.com/2024/12/04/

Iodixanol Purification of Porcine Islets • Sydney IPITA 1999 • 20241203

Slides oral presentation Michel van der Burg, IPITA congress 1999 in Sydney, Australia

A novel iodixanol density gradient for purification of pancreatic islets was developed in the difficult pig model. The pig islets restored normal blood sugar levels after xeno transplantation in diabetic mice.

Slideshow (pdf) oral presentation Michel van der Burg, IPITA congress 1999 in Sydney, Australia

Recovery of adult pig islets during isolation using Liberase vs collagenase-P, purification in OptiPrep-UWS, and culture for transplantation in nude mice • Sydney IPITA 1999 • Michel van der Burg • Miracles.Media • @1MEMO 20241203_2 • TakeNode 5f53ae49-6fb8-4bef-a434-ac2ce8151c2a • File 1MEMO_20241203_2

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Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Recovery of adult pig
islets during isolation using Liberase vs collagenase-P, purification in OptiPrep-UWS, and
culture for transplantation in nude mice. 7th World congress of the International Pancreas & Islet Transplant Association, Sydney (Australia) August 22–25, 1999

Citation info : Iodixanol Purification of Porcine Islets • Sydney IPITA 1999 • Michel van der Burg • Miracles.Media • 1MEMO 20241203 • TakeNode ea1f1abd-6227-4ae6-985b-f8188310cd24 • URL michelvanderburg.com/2024/12/03/

Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • 20241202

Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202_1

I started an archive at Archive.org November 11, 2010 with this slideshow (pdf) of my January 1999 oral presentation in Igls (Innsbruck), Austria, at the 18th Workshop of the AIDSPIT (Artificial Insulin Delivery Systems, Pancreas and Islet Transplantation) Study Group. (Now known as AIDPIT).

The presented results of this innovative islet purification method — developed over nearly 10 years (from 1989) in our Leiden islet laboratory (LUMC) — were the outcome of work I had done spring 1998 with the human islet transplantation group of Camillo Ricordi at the Diabetes Research Institute in Miami , Florida, USA.

Slideshow (pdf) Archive.org

Description

Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999

Slides oral presentation Michel van der Burg, Aidspit meeting 25 Jan 1999, in Igls, Austria

Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification. 18th Workshop Aidspit, Igls (Austria) Jan. 24-26, 1999

Program :
18TH WORKSHOP OF THE AIDSPIT STUDY GROUP – JANUARY 24-26, 1999 – IGLS/AUSTRIA . MONDAY, JAN 25, 1999 – KONGRESSHAUS IGLS, MAIN LECTURE HALL
SESSION 2 – ISLET ISOLATION AND TRANSPLANTATION
CHAIRMEN:K. FEDERLIN, GIESSEN, GERMANY – G. POZZA, MILAN, ITALY 9.00 – 10.30 FREE COMMUNICATIONS
9.45- Efficacy of the novel iodixanol-UWS density gradient for human islet purification.
Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C; Leiden, The Netherlands and Miami, USA

File : mvdb19990118_1534_AIDSPIT.pdf | Uploaded 20101111 and replaced by the same pdf with updated metadata of the document on 20101112 (see Notes section).

Citation info (Archive file) : Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202 • URL https://archive.org/details/EfficacyOfTheNovelIodixanol-uwsDensityGradientForHumanIslet • TakeNode 54cc8b66-672e-4b13-913c-449038ed0521

Video Slideshow

The slideshow video embedded below, was first posted in 2018 as Human Islet Purification Miami 1998 • 20181102

Notes

The slideshow pdf file is available for download at Archive.org. I advise against the other download options presented by Archive.org , which do not correctly represent the slideshow.

The file was also uploaded to Scribd at the same time – URL https://www.scribd.com/document/42197150/Efficacy-of-the-novel-iodixanol-UWS-density-gradient-for-human-islet-purification-michelvanderburg-19990125

Abstract reference

Van der Burg MPM, Ranuncoli A, Molano R, Kirlew T, Ringers J, Bouwman E, Ricordi C. Efficacy of the novel iodixanol-UWS density gradient for human islet purification (Abstract). Acta Diabetol 1998; 35: 247.

Citation info (this post) : Efficacy of the novel iodixanol-UWS density gradient for human islet purification • Igls Aidspit 1999 • Michel van der Burg • Miracles.Media • 20241202 • URL michelvanderburg.com/2024/12/02/

ADD Diabetes Group 1989, St Edmund Hall, Oxford • 20241019

St Edmund Hall, University of Oxford, July 1989 • Michel van der Burg • Miracles•Media • 20241019_1 • TakeNode af426345-4fb3-48a3-8d5e-4368c56826aa

Venue of the Seventh Annual Meeting of the Anglo–Danish–Dutch Diabetes Group*, Oxford (UK) July 5–9, 1989.

The first international meeting were our new concept was presented, of pancreatic islet isolation in the University of Wisconsin Solution (UW-Solution or UWS) a solution designed recently for preservation of a donor pancreas during cold storage prior to transplantation.

Quote :


“…A few months ago, we adopted an entirely new approach to islet isolation.
Recently a new cold storage organ preservation solution was developed at the University of Wisconsin, the UW solution, allowing long term, 24 to 72 h, cold storage of the canine pancreas.
Since islet isolation is largely performed in the cold, we tested this preservation solution as the isolation medium throughout the isolation procedure. This slide shows that using the UW solution, purity has increased to over 90%…”

In addition our first successful pre-clinical transplant, we had performed the last month, was reported as breaking news at the end of the talk.

Quote :

“Three weeks ago we did our first autotransplantation of isolated islets from the whole gland by retrograde infusion into the spleen. The dog is doing fine: fasting glucose amounts to 5-6 mM and postprandial glucose between 6-8 mM ; postprandial insulin increases 6-7 fold. Thank you.”


St Edmund Hall, Oxford, 1989, 7th Meeting ADDD Group • Michel van der Burg • Miracles•Media • 20241019_2 • TakeNode ca40adf0-3b86-4ada-93a8-126c6768d420

Notes


Seventh Annual Meeting Anglo Danish Dutch Diabetes Group • Michel van der Burg • Miracles•Media • 20241019_3 • TakeNode bffdea47-9464-4f7b-8299-ec299d41ca4d

* Group supported through the Nordisk UK Professional Programme.

Van der Burg MPM, Gooszen HG, Scherft JP, Terpstra JL, Frölich M, Guicherit OR, Lemkes HHPJ, Bruijn JA. The isolation of canine pancreatic islets for transplantation. Seventh Annual Meeting Anglo Danish Dutch Diabetes Group, Oxford (UK) July 5–9, 1989.

Van der Burg MPM, Gooszen HG, Guicherit OR, Jansen JBMJ, Frölich M, Lamers CBHW. The mechanism of pancreatic duct obliteration induced hyperglycemia. Seventh Annual Meeting Anglo Danish Dutch Diabetes Group, Oxford (UK) July 5–9, 1989.

Citation info : ADD Diabetes Group 1989, St Edmund Hall, Oxford • Michel van der Burg • Miracles•Media • 20241019 • URL 1-memo•com

Pittsburgh 1992 Cell Transplant Society • 20240812

Poster Cell Transplant Society, Pittsburgh (USA) June 3, 1992 • 20240812_1 • michelvanderburg•com • TakeNode ca498136-3e99-4486-b641-f9f61894f19c

Congress

Pittsburgh (USA) May 31–June 3, 1992. Founding meeting, and 1st Int. Congress of the Cell Transplant Society.

Michel van der Burg, Co-chair ‘Methods’ Poster Discussion CTS , Pittsburgh June 3, 1992 • 20240812_2 • michelvanderburg•com • TakeNode 509ce973-d218-4f9c-84ef-ffa7c4fdb0d1

Poster creation

The poster was designed and produced May 1992 on a Macintosh computer using MacDraw II (with bar graphs from Cricket Graph) and printed landscape across a chain of several sheets of paper using the Imagewriter dotmatrix printer.

For this 2024 remastered poster below, the 1992 MacDraw II file was converted and edited using LibreOffice Vanilla, and next Pixelmator Pro was used for additional ‘repair’ (conversion issues with the many layers used for ‘tubes’ creation) and the insertion of the photo scans.

Poster Pittsburgh 1992 Cell Transplant Society, remastered • 20240812_3 • michelvanderburg•com • TakeNode 26159e06-9f29-4fab-a5f3-a595469b352e

Notes

Van der Burg MPM, Guicherit OR, Scherft JP, Bruijn JA, Frölich M, Gooszen HG. Islet preservation during isolation: a new concept in cell transplantation. First International Congress of the Cell Transplant Society, Pittsburgh (USA) May 31 – June 3, 1992.

Van der Burg MPM, Guicherit OR, Frölich M, Bruijn JA, Gooszen HG. Islet preservation during isolation: a new concept in cell transplantation. Transplant Proc. 1992 Dec;24(6):2840-1. PMID: 1465965. PDF FILE available below
PMID_1465965 🔗

Updated 20250115 : proceedings paper added

Citation info : Pittsburgh 1992 Cell Transplant Society • 20240812 • Michel van der Burg • michelvanderburg•com

Updates • 20240808

Lyon 1991 Percoll Poster | 20240731 • 20240808 | Michel van der Burg | michelvanderburg•com | TakeNode 8cd3161a-2405-4586-aaeb-08096e1a636e

Two posts have been updated / upgraded :

  1. Leiden Islet Laboratory History ( 20240803 ) was updated last night , by adding some history notes. | URL https://michelvanderburg.com/2024/08/03/leiden-islet-laboratory-history-20240803/
  2. Percoll UWS Pancreatic Islet Purification (20240731) now has a remastered poster image , see below.

Lyon 1991 Percoll Poster | 20240731 • 20240808

20240808 – remastered poster : Following the first publication here last month (20240731) of the original 1991 MacDraw poster opened (converted) in LibreOffice Vanilla (1), I learned how to do some editing in the LibreOffice app to further restore poster layout details such as line spacing, and font style. An archive photo of another poster presentation in Pittsburgh in 1992 (that will be posted here soon) guided this restoration.

Current workflow :
1) opening the MacDraw II file in LibreOffice Vanilla
2) editing in in LibreOffice Vanilla , saving as LibreOffice Vanilla file (OpenDocument Drawing – extension odg).
3) opening the photo tiff image in the Mac’s Preview app, and copying the displayed image to the clipboard
4) pasting the clipboard image in the LibreOffice doc , and dragging to adjust size and location 5) exporting the file as PDF (image resolution 300 DPI ; PDF/A-1b ; PDF/UA). 6) Converting the PDF in the Preview app to TIFF 144 pixels/inch. 7) Adding caption , metadata etc with JPEG conversion in PhotoMill.

The 20240731 post now has this updated 20240808 – poster image.

Citation info : Lyon 1991 Percoll Poster | 20240731 • 20240808 | Michel van der Burg | michelvanderburg•com | TakeNode 8cd3161a-2405-4586-aaeb-08096e1a636e

Minneapolis 1989 Poster | 20240804

Minneapolis 1989 Poster • 20240804 • michelvanderburg•com

September 1989 at a symposium of two international societies in Minneapolis, USA (1) , I presented this first poster on the ‘ invention ‘ of using the UW solution ( the University of Wisconsin organ preservation solution) during pancreatic islet isolation, with the exciting discovery of near-complete purification of islets of Langerhans in the laboratory for transplantation in diabetic patients.

Here the remastered 20240804 edition of that Minneapolis 1989 poster presentation.

The UWS data had been added ad hoc in the poster – that is , it wasn’t part of the accepted abstract. The first proceedings paper on our UWS work was also submitted at this meeting , and was published April ’90 (3).

A previous edition of the remastered poster was published here two years ago (2) but that edition had a different photo print (higher magnification, and without the bar indicator) of the purified islet preparation inserted, not the original image. This has been corrected now. In addition the original line spacing is now restored.

Notes

1) Van der Burg MPM, Gooszen HG, Ploeg RJ, Scherft JP, Field MJ, Guicherit OR, Terpstra JL, Van de Woude FT, Frölich M, Bruijn JA. Comparison of islet isolation techniques in dogs: Over 90% purified islets using UW solution. 4th International Symposium on Organ Procurement and Preservation and 2nd International Congress on Pancreatic and Islet Transplantation, Minneapolis (USA) September 17–22, 1989.

2) Minneapolis 1989 Poster UW Solution Islet Isolation | 20220822 | Michel van der Burg | michelvanderburg•com | URL https://michelvanderburg.com/2022/08/22

3). Van der Burg MPM, Gooszen HG, Ploeg RJ, Scherft JP, Field MJ, Guicherit OR, Terpstra JL, Van de Woude FJ, Frölich M, Bruijn JA. Comparison of islet isolation techniques in dogs: Over 90% purified islets using UW solution. Transplant Proc. 1990 Apr;22(2):795-6. PMID: 2158174.
This paper appears to be not readily available online – a reprint copy is available here PMID_2158174 🔗

PMID_2158174Download

Updates

20241211 : Minor text updates 

20260128 : Added Reference 3 and pdf file of the proceedings paper

Citation info : Minneapolis 1989 Poster | 20240804 | Michel van der Burg | michelvanderburg•com | TakeNode 91d70715-3fb3-4380-906a-0daf1b5ddd6a