Minneapolis 1989 Poster UW Solution Islet Isolation | 20220822

Minneapolis 1989 Poster • 20240804 • michelvanderburg•com

September 1989 at a symposium of two international societies in Minneapolis, USA (1) , I presented this first poster on the ‘ invention ‘ of using the UW solution ( the University of Wisconsin organ preservation solution) during pancreatic islet isolation, with the exciting discovery of near-complete purification of islets of Langerhans in the laboratory for transplantation in diabetic patients.

The next month at a meeting in Barcelona, Spain, this rather radical new concept was introduced (2, 3). June 1989 submission for publication as a letter in the Lancet had had no succes ;)

Poster designed and produced on my personal Macintosh ED and Imagewriter-II matrix printer, allowing full width printing over multiple pages in landscape (a low cost do-it-yourself discovery by me, at a time when posters were prepared on poster boards by graphical professionals using photography and special lettering techniques for around $400). Original MacDraw II file conversion with LibreOffice Vanilla.

Notes

1) Van der Burg MPM, Gooszen HG, Ploeg RJ, Scherft JP, Field MJ, Guicherit OR, Terpstra JL, Van de Woude FT, Frölich M, Bruijn JA. Comparison of islet isolation techniques in dogs: Over 90% purified islets using UW solution. 4th International Symposium on Organ Procurement and Preservation and 2nd International Congress on Pancreatic and Islet Transplantation, Minneapolis (USA) September 17–22, 1989.

2) Van der Burg MPM, Gooszen HG, Ploeg RJ, Guicherit OR, Scherft JP, Terpstra JL, Bruijn JA, Frölich M. Pancreatic islet isolation with UW solution: a new concept. 4th Congress of the European Society for Organ Transplantation, Barcelona (Spain) November 1–4, 1989.

3) Van der Burg MPM, Gooszen HG, Ploeg RJ, Guicherit OR, Scherft JP, Terpstra JL, Bruijn JA, Frölich M. Pancreatic islet isolation with UW solution: a new concept. Transplant Proc 1990; 22: 2050–2051.

Updates

20240804 – This remastered 20240804 edition of the Minneapolis 1989 poster , now shows the original photo of the islet prep as posted at the meeting in 1989. In addition the correct line spacing is now restored.
The previous poster published online two years ago (20220822) showed a different photo print (higher magnification, and without the bar indicator) of the same islet prep, not the correct image.

20241211 – Minor text updates

Citation info : Minneapolis 1989 Poster UW Solution Islet Isolation | 20220822 | Michel van der Burg | michelvanderburg•com | TakeNode 91d70715-3fb3-4380-906a-0daf1b5ddd6a

Human Islet Purification Miami 1998 • 20181102

Efficacy of the novel iodixanol-UWS density gradient for human islet purification

Slideshow (video) of my January 1999 oral presentation in Igls (Innsbruck), Austria, at the 18th Workshop of the AIDSPIT (Artificial Insulin Delivery Systems, Pancreas and Islet Transplantation) Study Group. (Now known as AIDPIT).

These results of this innovative islet purification method developed in our Leiden islet laboratory (LUMC) were the outcome of work I had done spring 1998 with the islet group of Camillo Ricordi in the “fast track” center for diabetes research at the Diabetes Research Institute in Miami , Florida, USA.

Notes

The slideshow (pdf) was first uploaded Nov. 11, 2010 at at Scribd , and Archive.org • URL http://archive.org/details/EfficacyOfTheNovelIodixanol-uwsDensityGradientForHumanIslet

Original video title (file name) : 20181102 ~ Human Islet Purification Miami 1998

Updates:
20241202 – Text updated with extra info. Youtube video in post replaced by Vimeo edition. Copied 1-memo post to michelvanderburg.com .

Citation info : Human Islet Purification Miami 1998 • Michel van der Burg • Miracles.Media • 20181102 • URL michelvanderburg.com/2018/11/02/ • TakeNode 3f2ec4d2-afe0-4580-b055-92a2f231a4fa

Out Of Service | 20140911

Out Of Service | 20140911 | 1-memo•com | In the morning of 9/11 2001 logistics forced me to officially announce a temporarily – 1 week – ‘out of service’ of our Leiden islet isolation and transplant center. Our center was started earlier that summer of 2001 at the Leiden University Medical Center. So, I decided to have a short vacation abroad – heaven, after being on call 24/7 almost non stop for years. Later that day at home, I was downstairs watching TV — because upstairs near my workroom the central heating was being replaced – when I saw the CNN breaking news about the plane crashing into the World Trade Center. That day would become a landmark in history and the course of my life.

Background image : Microscopy picture I took of purified human Islets of Langerhans – tiny clumps (size around 1/10 mm or 1/25 inch) of 10s to 1000s of cells that produce hormones like insulin – these cells are just visible when you enlarge this 50x microscopy picture : as tiny translucent ‘balls’ or ‘spheres’. The red color is from a stain that binds to the insulin cells.
Iodixanol-UWS purified human islets, stained with dithizone. Original magnification 50x.

Title: mvdb20041118_114555m0400.tif
Creator: Michael P.M. van der Burg
Caption/Description: Iodixanol-UWS purified human islets, stained with dithizone. Original magnification 50x. Copyright Notice: Copyright © 2004 M.P.M. van der Burg. All rights reserved. Digital Creation Date: 18 Nov 2004

References

Image published in : Transplantatie van eilandjes van Langerhans bij patiënten met diabetes mellitus type 1. Ned Tijdschr Geneeskd 2005 1 januari;149(1) – URL https://www.ntvg.nl/system/files/publications/2005100120001a.pdf

Efficacy of the novel iodixanol-UWS density gradient for human islet purification (Presentation publ. Archive Org – michelvanderburg, 19990125 )
https://archive.org/details/EfficacyOfTheNovelIodixanol-uwsDensityGradientForHumanIslet

25 Years of the Ricordi Automated Method for Islet Isolation. Piemonti L, Pileggi A. in CellR4 2013; 1(1): e128. – URL https://www.cellr4.org/article/128

Credit : Out Of Service | 20140911 | 1-memo•com | TakeNode ID e22f209c-14da-40d8-b11a-74a29b1e80b4

TAGS #michelvanderburg #1Memo #oncall #LeidenUniversity #OutOfService #Islet #Langerhans #isolation #purification #transplantation #clinic #human #center #diabetes #CNN #WorldTradeCenter #breakingnews #crash #CV #LUMC #microscopy #dithizone #stain #cell #insulin #therapy #Iodixanol #UWS #UniversityofWisconsin #solution

25 Year Islet Transplants • @1MEMO_20140703

17:35 – Michel enters with transplant.

17:50 – Vygon 6 cannulation

17:52 – 12 mg Heparin IV

17:57 – Transplant

18:00 – 2nd transplant
Activated Clotting Time (ACT) 362″

18:05 – Clamps removed

25 Year Islet Transplants • @1MEMO_20140703 • Michel van der Burg • Miracles•Media • TakeNode c28e9c36-22bc-4409-b26a-2f9f90d9d39b • July 3, 2014 – Holland, Leiden University Hospital, 25 years ago : Photocopy June 13, 1989 surgery journal highlighting the moment of islet transplantion at our start of a first series of succesfull islets graft transplants in the Netherlands, using a simple yet very novel approach that eventually, recently, became a worldwide standard for transplanting clinical grade purified islets in insulin-dependent diabetic patients.
Link to article on the 25 year timeline of progress in research and patient treatment , here : https://www.cellr4.org/article/128 .

Notes

Update 20260319_1021 : adding english translation dutch surgery journal (as quote), and minor text modifications, as part of the process of republishing this post at michelvanderburg.com as part of the science portfolio dd 20260319 https://michelvanderburg.com/category/science/ from the original post at 1-memo•com: 25 Year Islet Transplants | 20140703 | 1-memo.com • URL https://1-memo.com/2014/07/03/25-year-islet-transplants/

#islet #transplantation #diabetes #LUMC #Leiden #history #AZL #UWS #purification #premiere