Tag: canine

Quantification of canine pancreatic islet isolation • 1st IPITA 1988 • @1MEMO 20251206

Quantification of canine pancreatic islet isolation • 1st IPITA , Stockholm (Sweden) March 27–29 1988 • Digitally remastered by author, publisher, Michel van der Burg • Miracles•Media • 1MEMO_20251206_01 • TakeNode 430989b2-52f0-4e36-8dbd-cf7bf030668c • #IEQ #diabetes #islets #transplant #methods #LUMC #IPITA

After founding the Leiden Islet Laboratory (1) in the University Hospital Leiden in Holland (later known as LUMC), and reporting the outcome of our first year of canine islet research at a national meeting November 1987 (2), next, this first poster ‘Quantification of canine pancreatic islet isolation’ for a world congress was presented March 1988 at the First International Congress on Pancreatic and Islet Transplantation (IPITA)* in Stockholm, Sweden (3,4).

This pilot work introduced our novel approach of volume quantification of islet isolation outcome by measuring the volume of the isolated islets . In addition we compared the outcome with the total volume of islets measured in the canine pancreas.
Note, that a similar approach, using islet volume measurements for the quantification in islet isolation assessment in man and large animals, was proposed the next year in an International Workshop during the 2nd Congress on Pancreas and Islet Transplantation, Sep. 1989, in Minneapolis, by Camillo Ricordi and some 150 participants from 30 institutes actively involved in islet isolation and transplantation — proposing a procedure for standardized islet volume measurements (based on the number of islets in diameter classes incrementing 50 µm) and reporting outcome in Equivalent Islet Numbers (total volume expressed in the equivalent number of spheres with a 150 µm diameter; abbreviated as EIN, currently known as the number of IEQs (Islet EQuivalents), which greatly enhanced islet research (5).

Unfortunately back problems prevented me to attend that 1st IPITA congress, I was looking forward to, but, fortunately, the poster was presented by my colleagues Hein Gooszen (Surgery Department) and Marijke Frölich (Chemical Pathology Department) of the University Hospital Leiden. Ms. Marijke Frölich also presented a nice poster (7,8) on our quantification of the islets cells , the hormones, in the right lobe (aka , the duodenal segment , the ventral lobe) and left lobe of the canine pancreas (aka, the body and distal tail , the dorsal lobe) of the beagle pancreas , showing the diversity of the islets in these two segments of the pancreas — corresponding to the head and tail of the human pancreas, respectively.
Below the excellent airbrush work by an artist from the Audiovisual Services of Leiden University, for our poster ‘Distribution of insulin, glucagon, pancreatic polypeptide and somatostatin in the canine pancreas’.

Airbrush art on poster board created by the Audiovisual Services of Leiden University. Showing the location of pancreatic tissue sampled from the proximal and distal part of the right and left lobe of the beagle pancreas. The hormone values did not differ significantly between the proximal and distal parts of each lobe. Michel van der Burg • Miracles•Media • 1MEMO_20251206_02 • TakeNode 10b1d696-9d7f-4fa3-a8f9-7a3211546f24


Poster board showing the distribution of the four different types of islet cells by assessment of the hormones insulin, glucagon, pancreatic polypeptide, and somatostatin between the right lobe (RL) and left lobe (LL) of the canine pancreas. The number of insulin, glucagon, and PP producing islet cells differs considerably between both lobes. Michel van der Burg • Miracles•Media • 1MEMO_20251206_03 • TakeNode 47333616-b85d-404e-b103-2ccb3b86d406

I created the original 1988 poster ‘Quantification of canine pancreatic islet isolation’ on March 9-10, 1988 using MacDraw on a personal Macintosh ED , using the bold Chicago font, and the Imagewriter-II matrix printer , except for the header provided by my colleagues , which was together with my colleagues’ posters designed and produced as usual at the time on rigid foam boards by the Audiovisual Services department of the Leiden University. Also the original photo print was pinned on the mounted poster (thus, not digitally processed at the time).
This digital remastered 2025 edition (1MEMO_20251206_1) was produced in LibreOffice Vanilla with conversion of the original 1988 MacDraw file, correcting the disrupted formatting, replacing the non-available Chicago font with the font ‘Chalkboard’ , adding a scan of the Figure 1 photo print , plus a recent photo taken of the original poster board, archived, header. Figure 1 uses a copy of that original photo print , with the addition now, in this remastered edition, of arrows helping identifying the islets , and addition of the text ‘(arrrows)’ in the figure legend.

Other papers presented at the 1st IPITA congress from our research group, on pancreas transplantation, are listed below (9,10,11,12).

David Sutherland

Several other centers reported at this 1st IPITA on succesful islet autotransplantation in large animals, including a series of presentations on canine islet transplantation from David Sutherland’s islet research group in Minneapolis.

We had already started collaborating, by the end of 1987, with David Sutherland and Ms. Jane Field , the head of Sutherland’s islet research laboratory in Minneapolis , planning Jane’s working visit for June 1988 in our Leiden Islet Laboratory, in order to help me set up in Leiden, the current Minneapolis procedure for canine islet isolation and transplantation (13,14).

The next year, Sep-Oct 1989, I worked again with Jane Field and David Sutherland, now in Minneapolis (14,15,16) in the islet research laboratory mostly, and also observing David Sutherland at work during clinical segmental pancreas autotransplantaton, where I made this portrait photograph (1MEMO_20251206_06).

David Sutherland • Clinical Pancreas Transplant, Minneapolis, 1989 • Michel van der Burg • Miracles.Media • 1MEMO_20251206_06 • TakeNode f4769bdf-c7a8-436a-bc5c-2d1505226c05 • David Sutherland performing a clinical segmental pancreas autotransplantation, Minneapolis, 1989. Photo by Michel van der Burg . URL https://michelvanderburg.com/2025/12/06

David Sutherland, the ‘father’ of pancreas and islet transplantation, passed away March 25, 2025.

David jokingly called the pancreas the “Big Dirty Island”.

Notes

* The founding meeting of the IPITA was being held actually at the 4th IPITA meeting — the 4th International Congress on Pancreas and Islet Transplantation — June 27–30, 1993 in Amsterdam (The Netherlands) hosted by Dr. Reinout van Schilfgaarde.

1. Leiden Islet Laboratory History • 20240803 • Michel van der Burg • michelvanderburg•com • URL https://michelvanderburg.com/2024/08/03/leiden-islet-laboratory-history-20240803/

2. Cradle of Islet Transplants • 20240829 • Michel van der Burg • michelvanderburg•com • https://michelvanderburg.com/2024/08/29/cradle-of-islet-transplants-20240829/

3. Van der Burg MPM, Gooszen HG, Terpstra JL, Frölich M, Scherft JP, Lemkes HHPJ. Quantification of canine pancreatic islet isolation. First International Congress on Pancreatic and Islet Transplantation, Stockholm (Sweden) March 27–29, 1988. (Poster). Remastered 2025 poster edition : 1MEMO_20251206_01.

4. Van der Burg MPM, Gooszen HG, Terpstra JL, Frölich M, Scherft JP, Lemkes HHPJ. Quantification of canine pancreatic islet isolation (Abstract). Diabetes 1989; 38 (Suppl. 1): 274. (Abstract). View/Download file 1MEMO_20251206_04

1MEMO_20251206_04Download

5. Quantitative And Qualitative Standards For Islet Isolation Assessment In Man And Large Mammals. International Workshop during the 2nd Congress on Pancreas and Islet Transplantation. Camillo Ricordi. Archive Michel van der Burg • Miracles•Media • 1MEMO_20251206_07 • TakeNode 3d90aa54-9fe1-4b29-8161-aa6c4fda6652 • View/Download file 1MEMO_20251206_07

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6. Ricordi C, Gray DW, Hering BJ, Kaufman DB, Warnock GL, Kneteman NM, Lake SP, London NJ, Socci C, Alejandro R, et al. Islet isolation assessment in man and large animals. Acta Diabetol Lat. 1990 Jul-Sep;27(3):185-95. doi: 10.1007/BF02581331. PMID: 2075782.

7. Frölich M, Van der Burg MPM, Gooszen HG, Jansen JBMJ, Lamers CBHW. Distribution of insulin, glucagon, pancreatic polypeptide and somatostatin in the canine pancreas. First International Congress on Pancreatic and Islet Transplantation, Stockholm, Sweden, March 27–29 1988. (Poster).

8. Frölich M, Van der Burg MPM, Gooszen HG, Jansen JBMJ, Lamers CBHW. Distribution of insulin, glucagon, pancreatic polypeptide, and somatostatin in canine pancreas (Abstract). Diabetes 1989; 38 (Suppl. 1): 257. (Abstract) . Download file 1MEMO_20251206_04

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9. Gooszen HG, Van der Burg MPM, Guicherit OR, Jansen JBMJ, Van Schilfgaarde R, Lamers CBHW, Frölich M. A crossover study on the effects of duct obliteration, coeliac denervation and autotransplantation on glucose and meal stimulated insulin, glucagon and pancreatic polypeptide levels. First International Congress on Pancreatic and Islet Transplantation, Stockholm, Sweden, March 27–29 1988. (Talk).

10. Gooszen HG, van der Burg MP, Guicherit OR, Jansen JB, Frölich M, van Schilfgaarde R, Lamers CB. Crossover study on effects of duct obliteration, celiac denervation, and autotransplantation on glucose- and meal-stimulated insulin, glucagon, and pancreatic polypeptide levels. Diabetes. 1989 Jan;38 Suppl 1:114-6. doi: 10.2337/diab.38.1.s114. PMID: 2642831. (Proceedings paper).

11. Gooszen HG, Van Schilfgaarde R, Van der Burg MPM, van Lawick van Pabst WP, Frölich M, Bosman FT. Quantitative assessment of changes in insulin secretion after canine ductobliterated segmental pancreatic autotransplantation in relation to the histological background. First International Congress on Pancreatic and Islet Transplantation, Stockholm, Sweden, March 27–29 1988. (Poster).

12. Gooszen HG, Van Schilfgaarde R, Van der Burg MPM, Van Lawick van Pabst WP, Frölich M, Bosman FT. Quantitative assessment of changes in insulin secretion after canine duct-obliterated segmental-pancreas autotransplantation in relation to histological background (Abstract). Diabetes 1989; 38 (Suppl. 1): 255–256. (Abstract). View/Download file 1MEMO_20251206_05

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13. December 1987 , we started planning together Jane Field’s working visit thanks to our nephrologist Fokko van der Woude who after post doc research in Minneapolis had just started as an associate Professor at the Leiden University , working in our Leiden University Hospital (LUMC). Fokko J. van der Woude (1953-2006) passed away too soon after a long battle with cancer.

14. Pancreas & Islet Transplantation Program • 20240912 • Michel van der Burg • michelvanderburg•com • URL https://michelvanderburg.com/2024/09/12/pancreas-islet-transplantation-program-20240912/

15. Visiting research fellow in the Department of Surgery at the University of Minnesota (DER Sutherland, MD PhD) & Department of Cell Biology (OD Hegre, MD PhD), University of Minnesota, Minneapolis Minnesota (USA) , September 14-October 6 1989

16. 2nd International Congress on Pancreatic and Islet Transplantation, and 4th International Symposium on Organ Procurement and Preservation. Minneapolis (USA) September 17–22, 1989.

Citation info : Quantification of canine pancreatic islet isolation • 1st IPITA 1988 • Michel van der Burg • Miracles.Media • @1MEMO 20251206 • URL https://michelvanderburg.com/2025/12/06

Assessment of isolated islet equivalents • Cell Transplant Society 1996 • @1MEMO 20251205


Assessment of isolated islet equivalents • Cell Transplant Society 1996 • Michel van der Burg • Miracles.Media • 1MEMO_20251205_1 • Poster 3rd International Congress Cell Transplant Society, Miami (USA) Sep 29 – Oct 2, 1996


Assessment of isolated islet equivalents • Cell Transplant Society 1996 • Michel van der Burg • Miracles.Media • 1MEMO_20251205_1 • Poster 3rd International Congress Cell Transplant Society, Miami (USA) Sep 29 – Oct 2, 1996 • TakeNode 1300e7cd-85a4-4cd0-9cbc-a88746e625ab • #IEQ #diabetes #islets #transplant #methods #CTRMS

IEQ – Islet Equivalent

IEQ islet stands for Islet Equivalent, a standard unit used to measure the total volume of islets (clusters of insulin cells and other hormone-producing cells in the pancreas). It is defined as a single spherical islet with a diameter of 150 µm. The IEQ is a crucial parameter for assessing the quantity of islets for procedures like clinical islet transplantation, which is a treatment for type 1 diabetes.

Modified international procedure

Following a consensus report in 1989 – 1990 by Ricordi et al (1a, 1b) on islet isolation assessment, the total volume of isolated islets is generally expressed in the number of islet equivalents (IEQs) — defined as islets of 150 µm diameter— and many centers use the advocated now classic international procedure (CIP) of sizing all islets in classes with 50-µm increments (i.e., 50–100, 100–150, etc.) for calculation of the number of IEQs. For each class the number of IEQs are calculated by multiplying the islet counts with a conversion factor — based on the mean volume of that class.

A modified international procedure — based on our conventional Leiden procedure (2,3) with islet sizing in classes with 25-µm increments — is suggested for accurate assessment of the IEQs especially when isolation yields mostly smaller islets, in our paper ‘Assessment of isolated islet equivalents’ presented at the 3rd International Congress Cell Transplant Society, Miami (USA) Sep 29 – Oct 2, 1996, in both a poster (4) , and proceedings paper (5). A photo of the original poster , (retrieved somewhat wrinkled 30 years later from my archive), is posted here, as well as a pdf from the (corrected proof) manuscript of the proceedings paper below (6).

The Cell Transplant Society was founded in 1991, and has been renamed the Cell Transplant and Regenerative Medicine Society (CTRMS).

Notes

1a. Ricordi C, Gray DW, Hering BJ, Kaufman DB, Warnock GL, Kneteman NM, Lake SP, London NJ, Socci C, Alejandro R, et al. Islet isolation assessment in man and large animals. Acta Diabetol Lat. 1990 Jul-Sep;27(3):185-95. doi: 10.1007/BF02581331. PMID: 2075782.

1b. Quantitative And Qualitative Standards For Islet Isolation Assessment In Man And Large Mammals. International Workshop during the 2nd Congress on Pancreas and Islet Transplantation (1989). Camillo Ricordi. Archive Michel van der Burg • Miracles•Media • 1MEMO_20251206_07 . Available from Dec 6, 2025 URL https://michelvanderburg.com/2025/12/06/

2. Van der Burg MPM, Guicherit OR, Frölich M, Scherft JP, Prins FA, Bruijn JA, Gooszen HG. Assessment of islet isolation efficacy in dogs. Cell Transplant. 1994 Jan-Feb;3(1):91-101. doi: 10.1177/096368979400300113. PMID: 8162296.

3. Paper (Notes 2) included as Chapter 3 in my doctoral thesis (Leiden University, November 1994) : Assessment of islet isolation efficacy in dogs. In : Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation ( Chapter 3). (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Nov 24th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 . Direct link to file Chapter 3 , URL https://scholarlypublications.universiteitleiden.nl/access/item%3A3486610/view

4. Van der Burg MPM, Scheringa M, Basir I, Bouwman E. Assessment of isolated islet equivalents. Poster presented at the Third Int. Congress Cell Transplant Soc., Miami (USA) Sept. 29 – Oct. 2, 1996.
In : Assessment of isolated islet equivalents • Cell Transplant Society 1996 • Michel van der Burg • Miracles.Media • @1MEMO 20251205 • URL https://michelvanderburg.com/2025/12/05 • File 1MEMO_20251205_1 • TakeNode 1300e7cd-85a4-4cd0-9cbc-a88746e625ab

5. Van der Burg MPM, Scheringa M, Basir I, Bouwman E. Assessment of isolated islet equivalents. Transplant Proc. 1997 Jun;29(4):1971-1973. doi: 10.1016/s0041-1345(97)00188-7. PMID: 9193483.

6. Manuscript (corrected proof for Transplant Proc paper PMID: 9193483). Van der Burg MPM, Scheringa M, Basir I, Bouwman E. Assessment of isolated islet equivalents • 1MEMO_20251205_2 • TakeNode 48380f3c-b3f7-416e-90bb-ad6c53129e46 • PDF available below , File 1MEMO_20251205_2

1MEMO_20251205_2Download

#IEQ #diabetes #islets #transplant #methods #CTRMS

Citation info : Assessment of isolated islet equivalents • Cell Transplant Society 1996 • Michel van der Burg • Miracles.Media • @1MEMO 20251205 • TakeNode 1300e7cd-85a4-4cd0-9cbc-a88746e625ab / 48380f3c-b3f7-416e-90bb-ad6c53129e46 • URL https://michelvanderburg.com/2025/12/05

GLP-1 blood sugar regulation in islet transplants • AIDSPIT 1994 • @1MEMO 20251114

Poster 13th Workshop of the AIDSPIT Study Group , Igls (Austria) January 23–25, 1994• Digitally remastered by author, publisher, M.P.M. van der Burg • Miracles•Media • 20251114 • TakeNode 810cc881-d084-4d2e-b252-d432918b1946 • #GLP1

GLP-1 blood sugar regulation in islet transplants

GLP-1 (glucagon-like peptide-1) is now well-known from the anti-diabetic drugs that mimic the action of this natural hormone GLP-1, to treat type 2 diabetes and obesity. They work by stimulating insulin release, reducing glucagon secretion, slowing stomach emptying, and suppressing appetite, which leads to lower blood sugar and weight loss.

May 1992 we were the first to show direct potentiation of the insulin response from isolated islets by a (near-)physiological dose of the gut hormone GLP-1 during perifusion (in vitro) of canine islets at 7.5-10 mM glucose levels (1,2).

Jan 1994 we next showed this incretin effect of GLP-1 also after islet transplantation (in vivo, in dogs) in this poster (3) and abstract (4) presented at the 13th Workshop of the AIDSPIT (aka AIDPIT) Study Group , Igls, Austria, January 23–25, 1994.

Background of the work was posted earlier this year (5) and the work was published Nov 1994 in my PhD thesis Pancreatic islet transplantation , available online (6).

Notes

1) Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Terpstra JL, Gooszen HG. Function after intrasplenic transplantation of islets of Langerhans in dogs (translated from dutch: Functie na intralienale transplantatie van eilandjes van Langerhans in de hond). Surgeon days ’92 of the Dutch Surgery Society, Rotterdam (The Netherlands) May 21–22, 1992. In : Gut factors controlling pancreatic islets • 20240910 | Michel van der Burg | michelvanderburg•com URL https://michelvanderburg.com/2024/09/10/gut-factors-controlling-pancreatic-islets-20240910/

2) van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Pept. 1995 Dec 7;60(1):61-7. doi: 10.1016/0167-0115(95)00122-4. PMID: 8747785.

3) Metabolic control after canine islet transplantation. M.P.M. van der Burg, P.T.R. van Suylichem, O.R. Guicherit, J.H.M. van Deijnen, M. Frölich, and H.G. Gooszen (Universities of Leiden & Groningen, The Netherlands). Poster presented at the 13th Workshop of the AIDSPIT Study Group , Igls, Austria, January 23–25, 1994 •

4) Metabolic control after canine islet transplantation. Van der Burg M.P.M., Guicherit O.R., van Suylichem P.T.R., Frölich M., van Deijnen J.H.M. , Gooszen H.G. (Leiden, The Netherlands; Groningen, The Netherlands). Horm metab Res 1994; 26: 63. (Abstract). View/Download file 1MEMO_20251113_2

1MEMO_20251113_2Download

5) Background of this work was posted in Dutch Islets Transplant Team • @1MEMO 20250125 • URL https://michelvanderburg.com/2025/01/25/dutch-islets-transplant-team-1memo-20250125/

6) Michael P.M. van der Burg. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation. (Doctoral Thesis, Faculty of Medicine, Leiden University Medical Center (LUMC) , Leiden University), Boskoop: M.P.M. van der Burg | Miracles.Media (ISBN electronic, pdf, 9789080216402 ; ISBN print 9789080216419, 9080216410), 1994: 192 p. URL Thesis Repository Leiden University https://hdl.handle.net/1887/3486604

PM – Recording of the 13th AIDSPIT seminar on lessons from clinical islet transplantation was posted yesterday (7).

7) Clinical Islet Transplantation 13th AIDSPIT • @1MEMO 20251113 • URL https://michelvanderburg.com/2025/11/13/clinical-islet-transplantation-13th-aidspit-1memo-20251113/

#GLP1 #diabetes #islets #transplant #AIDSPIT

Updates

20251127 title updated – insert : AIDSPIT 1994

Citation info : GLP-1 blood sugar regulation in islet transplants • AIDSPIT 1994 • Michel van der Burg • Miracles.Media • @1MEMO 20251114 • TakeNode 810cc881-d084-4d2e-b252-d432918b1946 • URL https://michelvanderburg.com/2025/11/14

Clinical Islet Transplantation 13th AIDSPIT • @1MEMO 20251113


Seminar on lessons from clinical islet transplantation

Chairman K. Federlin (Giessen, Germany) opened the seminar Tuesday, January 25, 1994 and David Scharp began with his lesson from the Center in St. Louis (USA) at the 13th Workshop of the AIDSPIT * Study Group , Igls, Austria, January 23–25, 1994.

Notes


Video image: Iodixanol-UWS purified human islets, stained with dithizone (March 18, 1999) • Michel van der Burg , Leiden Islet Laboratory LUMC .

Second, remastered, edition created from the 1st video edition published 20170208 via Vimeo at 1-memo.com .

* AIDSPIT later known as AIDPIT.

Recorded with Olympus mini-tape dictaphone, and re-recorded from the speaker of the dictaphone.

My presentations at the event

Cell preservation in University of Wisconsin solution during isolation of canine islets of Langerhans. Van der Burg M.P.M., Guicherit O.R., Frölich M., Gooszen H.G. (Leiden, The Netherlands) . Horm metab Res 1994; 26: 63. (Abstract). File 1MEMO_20251113_2

Metabolic control after canine islet transplantation. Van der Burg M.P.M., Guicherit O.R., van Suylichem P.T.R., Frölich M., van Deijnen J.H.M. , Gooszen H.G. (Leiden, The Netherlands; Groningen, The Netherlands). Horm metab Res 1994; 26: 63. (Abstract). File 1MEMO_20251113_2

View/Download file 1MEMO_20251113_2

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Citation info : Clinical Islet Transplantation 13th AIDSPIT • Michel van der Burg • Miracles.Media • @1MEMO 20251113 • TakeNode 24209f1e-67d5-4540-9115-89efcc6090c9 • URL https://michelvanderburg.com/2025/11/13/clinical-islet-transplantation-13th-aidspit-1memo-20251113/

Transplants of UW-Isolated Islets • Transplantation Society 1990 Paper • @1MEMO 20250118

Congress of The Transplantation Society, Lobby of the Fairmont Hotel, San Francisco, California, USA, August 1990 • Michel van der Burg • Miracles.Media • @1MEMO 20250118_1 • TakeNode 4af71bca-edaa-4adb-9dce-556534844f5b

Following papers presented in 1989 demonstrating that over 90% pure islets are isolated from pancreases when using the UW organ preservation solution (1), the outcome of transplantation of highly purified UW-isolated islets was reported in a proceedings paper presented August 1990 at the XIII International Congress of The Transplantation Society in San Francisco, USA (2,3).
The Transplantation Proceedings paper, published Feb 1991 is now available online below (3).

Notes

1) Pancreatic Islet Isolation With UW Solution • A New Concept • Michel van der Burg • Miracles.Media • @1MEMO 20241224 • URL michelvanderburg.com/2024/12/24/

2) Van der Burg MPM, Guicherit OR, Ploeg RJ, Frölich M, Bruijn JA, Scherft JP, Gooszen HG.
Metabolic control after autotransplantation of highly purified canine pancreatic islets isolated in UW-solution. XIII International Congress of The Transplantation Society, San Francisco (USA) August 19–24, 1990.

3) van der Burg MPM, Guicherit OR, Ploeg RJ, Frölich M, Bruijn JA, Scherft JP, Gooszen HG. Metabolic control after autotransplantation of highly purified canine pancreatic islets isolated in UW solution. Transplant Proc. 1991 Feb;23(1 Pt 1):785-6. PMID: 1990691. PDF available below

PMID_1990691🔗Download

Citation info : Transplants of UW-Isolated Islets • Transplantation Society 1990 Paper • Michel van der Burg • Miracles.Media • @1MEMO 20250118 • URL michelvanderburg.com/2025/01/18/

Science in print is out of date ? @1MEMO 20250115

Front Page • 20250115_1 • Miracles.Media • Link to full text paper PMID_1465965 🔗

Yesterday, Jan 14th 2025, I posted a video of the slideshow and audio track of my talk on the innovative Percoll-UWS density gradient for the purification of canine and human pancreatic islets, at the 5th Congres European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991 (1,2).

That paper was presented the next year , in a poster discussion I co-chaired at the 1st International Congress of the Cell Transplant Society in Pittsburgh (USA) May 31–June 3, 1992 (3,4).
The conference proceedings paper of that meeting has been published in Transplantation Proceedings in 1992 (5).

The dutch publisher Elsevier, is publishing Transplantation Proceedings from 1997, and provides (restricted) access to full text online.

Science in print is out of date ?

Previous editions (before 1997) of the journal Transplantation Proceedings , were published by Appleton & Lange, like this 1992 paper (5), and full text is not found online. Also, I am afraid, many libraries have thrown away their stocks of print editions. I, myself, have not kept the bulky Transplantation Proceedings volumes I once bought … thrown away for lack of space , and what I kept is not within reach in practice, piled up in the attic, like the many hundreds of scientific slides…

A pdf copy of that 1992 paper is now available below (5) ;) And copies will be added in upcoming updates of those previous posts.

Notes

1) Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 5th Congres European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991.

2) Percoll UWS Purification Pancreatic Islets • ESOT 1991 Talk • Michel van der Burg • Miracles.Media • @1MEMO 20250114 • URL michelvanderburg.com/2025/10/14/

3) Van der Burg MPM, Guicherit OR, Scherft JP, Bruijn JA, Frölich M, Gooszen HG. Islet preservation during isolation: a new concept in cell transplantation. First International Congress of the Cell Transplant Society, Pittsburgh (USA) May 31 – June 3, 1992.

4) Pittsburgh 1992 Cell Transplant Society • 20240812 • Michel van der Burg • michelvanderburg•com URL https://michelvanderburg.com/2024/08/12/pittsburgh-1992-cell-transplant-society-20240812/

5) Van der Burg MPM, Guicherit OR, Frölich M, Bruijn JA, Gooszen HG. Islet preservation during isolation: a new concept in cell transplantation. Transplant Proc. 1992 Dec;24(6):2840-1. PMID: 1465965.

SCAN PDF FILE available below

PMID_1465965🔗Download

Citation info : Science in print is out of date ? • Michel van der Burg • Miracles.Media • @1MEMO 20250115 • URL michelvanderburg.com/2025/01/15/

Percoll UWS Purification Pancreatic Islets • ESOT 1991 Talk • @1MEMO 20250114


Percoll UWS Purification Pancreatic Islets • ESOT 1991 Talk • Michel van der Burg • Miracles.Media • @1MEMO 20250114 • TakeNode 459d71fd-069f-4f6d-9c8f-924ebf132222

Two years, after we introduced the UW-Solution ( UWS ) as the islet isolation solution at the 4th ESOT meeting (1,2) , this talk on the innovative Percoll-UWS density gradient for the purification of canine and human pancreatic islets, was presented at the 5th ESOT — Wednesday Oct 9 , 1991, at 2 PM at the 5th ESOT meeting in Maastricht, Netherlands (3) .

That 5th ESOT meeting was recorded on tape by Audio Archives Int. I purchased some tapes, including the tape of that afternoon session that had started with my talk — and found that the taping of this session had started a few minutes too late … during my talk, the moment I started presenting the Results.

That original tape is now included in this remastered slideshow of my talk presented in this video (20250114) here , starting at 2 min 51 sec.
Audio of the first part of my speech , missing in the original 5th ESOT tape, I have recorded this week (from the handout of the speech, written out at full lenght) and added as a voice over at the start of this remastered slideshow.


Cassette Tapes , Audio Archives Int, 5th ESOT • Michel van der Burg • Miracles.Media • @1MEMO 20250114_2 • TakeNode 97730a39-b3db-4f56-a08a-77c4d0406be0

Background

In November 1989, I had my first chance for a presentation at a major international conference, the 4th ESOT meeting in Barcelona, of a paper centered on the rationale for employing the University of Wisconsin (UW) organ preservation solution in pancreatic islet isolation.

We demonstrated in 1989, that the use of the UW-Solution ( UWS ) for isolation of canine pancreatic islets prior to ‘conventional’ dextran gradient purification, consistently results in a greatly improved purification outcome, of >90% purified islets, and successful transplantation of the canine islets.

Thus , the UWS was used as the isolation solution both before , and after the purification of islets — but not yet during the purification step, during centrifugation of the pancreatic tissue in a density gradient.

A conventional (Hanks’ balanced salts) solution was still being used for preparing density gradients with dextran in our laboratory.

Following our islet transplants series June-August 1989 , next a working visit in Minneapolis Sep-Oct ’89, and presentations at the Nov ’89 ESOT congress in Barcelona , we had started a new series of islet isolation experiments from December 1989.

During the first two isolations, Dec 1989, of this new series, I wondered whether the dextran gradient could be optimized , for example by layering the islet suspension in UWS (the pancreatic digest suspension) on top of the dextran bottom layer, or perhaps layering the digest on top of a bottom layer of dextran dissolved in UWS…

The new , much shorter, Labcraft density hydrometers, I had purchased during the Minneapolis visit Nov. 1989 , would greatly facilitate designing new density gradients.

The same lot of dextran (Sigma’s industrial-grade dextran) had been used for all these studies. That stock ran out, so a switch was made Jan 2, 1990 to using a new batch of that dextran powder, resulting however in a significantly impaired recovery of islets during purification (4).

Thus, designing a new density gradient became urgent January 1990.

Dextran-UWS Gradient • First density gradient in UW-Solution

March 1990 , new density gradient research was started during islet isolation , splitting the suspension of digested pancreatic tissue (‘digest’) for comparison of different density gradients, of either Sigma’s dextran, and Pharmacia’s essentially pure dextran T70 product in Hanks’ solution … and also the first ‘UW gradient’ tested : a dextran gradient in UW-Solution (4).

Percoll-UWS • Successful density gradient in UW-Solution

From April 1990, dextran T70 was used also in dextran-UW gradients, and islet purification research was further extended that month, by designing a density gradient of Percoll in UW Solution, and from May 1990, designing a Pentastarch-UW gradient. Posters on these new density gradients in UWS were presented June 1991 at the 3rd IPITA Congress in Lyon, France (4,5,6).

The paper presented in this 5th ESOT talk, was presented the next year (1992) again in full detail in a poster at the 1st meeting of the Cell Transplant Society in Pittsburgh , and a remastered edition was posted online recently (7).

Proceedings paper below (8).

Slides originally created on a Macintosh computer October 1991, using the MacDraw II application, with graphs from Cricket Graph. Those 1991 files were remastered this month using LibreOffice, as well as the likewise produced Pittsburgh poster for this video of the 5th ESOT talk.
Handout speech and report files originally created in the Mac Desk Accessory ‘Acta’, currently accessed by Opal.

Notes

1) Pancreatic Islet Isolation With UW Solution • A New Concept • Michel van der Burg • Miracles.Media • @1MEMO 20241224 • URL michelvanderburg.com/2024/12/24/

2) Pancreatic Islet Isolation in UW Solution & Transplantation • ESOT 1989 Talk • Michel van der Burg • Miracles.Media • @1MEMO 20241229 • URL michelvanderburg.com/2024/12/29/

3) Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 5th Congres European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991.

4) Dextran UWS Pancreatic Islet Purification • Michel van der Burg • Miracles.Media • @1MEMO 20250104 • URL michelvanderburg.com/2025/01/04/

5) Percoll UWS Pancreatic Islet Purification • 20240731 • Michel van der Burg • michelvanderburg•com

6) PentaStarch UWS Pancreatic Islet Purification • 20240802 • Michel van der Burg • michelvanderburg•com

7) Pittsburgh 1992 Cell Transplant Society • 20240812 • Michel van der Burg • michelvanderburg•com

8) Van der Burg MPM, Guicherit OR, Frölich M, Bruijn JA, Gooszen HG. Islet preservation during isolation: a new concept in cell transplantation. Transplant Proc. 1992 Dec;24(6):2840-1. PMID: 1465965. PDF available below
PMID_1465965 🔗

End Card

5th Congres European Society for Organ Transplantation, Maastricht (The Netherlands) October 9, 1991 14:00

0:07 Photo taken at 4th ESOT in Barcelona, 1989 (two years earlier)

0:06-2:51 Voice Over Michel van der Burg • Recorded Jan 11th, 2025 • Substitute for audio missing in original tape recording

2:51 Start Original Audio Tape 5th ESOT session by Audio Archives Int

6:52 Start Discussion • Chair : Reinout van Schilfgaarde (Groningen, Netherlands) • With contributions by Bernhard Hering (Giessen, Germany), R. Seelis (Edmonton, Canada), Derek Gray (Oxford, UK), and Reinout van Schilfgaarde (Groningen, Netherlands).

Citation info : Percoll UWS Purification Pancreatic Islets • ESOT 1991 Talk • Michel van der Burg • Miracles.Media • @1MEMO 20250114 • URL michelvanderburg.com/2025/01/14/