Coding 1995 Internet Access Macintosh • @1MEMO 20250515

Screenshot April 11, 1995 at 21:48 of the desktop with a Filemaker window (•Brieffile, correspondence) on my Apple Macintosh PowerBook 160. A PICT file image that I recently gained access to.
Desktop shows icon (renamed) SLIP of the InterSLIP application I used to get modem access (phone line) to the Leiden University gateway to internet, email, news, gopher, ftp etc .
I wrote the protocol (two scripts) required by this SLIP application to get access to the Leiden University gateway to the internet.
From May 1995 Leiden University distributed my scripts to the other Macintosh users of our University.

Below the two scripts published May 18, 1995 ….

Below the ‘Minimal dialing script’ :

! Original script by Fred Morris
! Modifications and comments by Adam C. Engst
! 21-Feb-94 = Added redial feature (ACE)

@originate
! Initialize mode from string in InterSLIP configuration
note “Initializing modem”
write “^4\13”
pause 20
! Dial modem from number in InterSLIP configuration
note “Dialing ^1”
write “atdt^1\13”
! Check only for BUSY or CONNECT, then redial or connect
matchstr 1 10 “BUSY”
matchstr 2 20 “CONNECT”
matchread 500

@label 10
! The line is busy, so let’s redial the modem indefinitely
! This will also redial after the (matchread 500) timeout if you get
endless rings
note “Line busy, redialing ^1”
! First hang up and reinitialize modem from string in InterSLIP configuration
write “+++”
pause 60
write “ath\13”
write “^4\13”
! Then go back and dial again using phone number in InterSLIP configuration
jump originate

@label 20
! Everything worked fine, you’re connected now
note “Dialed successfully”
exit 0

@answer
@hangup
! Hang up the modem and reinitialize from string in InterSLIP configuration
! The only way to get here is to manually click the Disconnect button
note “Hanging up modem”
write “+++”
pause 60
write “ath\13”
write “^4\13”
exit 0

Below the “FullCRIgateScript” for access via 278282 with password and
username check:

! InterSLIP gateway script for CRIgate with password access
! original script by Adam C. Engst
! modified by MvdBurg@RULLF2.MedFac.LeidenUniv.NL (MPM van der Burg)
!
@originate
!write “\13”
note “Waiting for prompt”
matchclr
matchstr 1 1 “Annex username: “
matchread 900
note “Gateway not responding!”
exit -1
!
@label 1
note “Sending user name”
write “^5\13”
matchclr
matchstr 1 2 “Annex password:”
matchread 900
note “Gateway not responding!”
exit -1
!
!
@label 2
note “Sending password”
write “^6\13”
matchclr
matchstr 1 4 “Permission granted”
matchstr 2 3 “Username/Password Incorrect”
matchread 900
jump 99
!
@label 3
note “Username/Password Incorrect”
jump 99
!
@label 4
note “Requesting SLIP”
matchclr
matchstr 1 5 “drive-in: “
matchread 900
jump 99
!
@label 5
write “slip\13”
matchclr
matchstr 1 10 “Switching to SLIP.”
matchstr 2 6 “CLI: Command not found.”
matchread 900
note “Cannot invoke SLIP mode”
jump 99
!
@label 6
note “Command not found”
jump 99
!
@label 10
note “Searching IP address”
matchclr
matchstr 1 25 “Your address is “
matchread 900
note “No AddressLine found”
jump 99
!
@label 25
note “SLIP address found”
matchclr
matchexp 1 30 “[0-9][0-9]\.[0-9][0-9]\.[0-9][0-9]\.[0-9][0-9]\.”
matchread 500
note “No IP address found”
jump 99
!
@label 99
pause 1
pause 60
exit -1
!
! We’ve found an IP address, so inform MacTCP of it.
!
@label 30
note “Setting IP address”
setip “^0”
note “^0”
Pause 30
write “set port internet slip enable”
write “\13”
!
note “Slip enabled”
pause 20
!
matchclr
exit 0

!
! Answer and Hangup modes are currently unused in gateway
! scripts, but just for future compatibility, exit successfully.
!
@answer
@hangup
! Hang up the modem and reinitialize from string in InterSLIP configuration
! The only way to get here is to manually click the Disconnect button
note “Hanging up modem”
write “+++”
pause 60
write “ath\13”
write “^4\13”
exit 0

TAGs :
#coding #script #modem #dial #Leiden #University #SLIP #Apple #Powerbook #Mosaic #internet #history #desktop #intransition

Citation info : Coding 1995 Internet Access Macintosh • Michel van der Burg • Miracles•Media • @1MEMO 20250515 • TakeNode • URL 1-memo.com/2025/05/15 •

Islet Transplants Performance 5th ESOT 1991 • @1MEMO 20250109

Islet Transplant Performance 5th ESOT 1991 • Michel van der Burg • Miracles.Media • @1MEMO 20250109_1 • TakeNode 75edbb4e-4360-43b8-8783-363ffd34c0ef

Poster (1) presented at the 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991. The poster was originally created on a Macintosh computer Oct 6, 1991, using the MacDraw II application, with graphs from Cricket Graph. That 1991 poster was remastered today in LibreOffice.

We presented 2 posters at the 5th ESOT, this poster (1), and another one on the effects of denervation with pancreas transplantation (2).

Two years after the 4th ESOT (in Barcelona, Nov 1989) where we introduced the UW-Solution as an islet isolation solution before density gradient purification, we now had a talk at this 5th ESOT on the use of the UW-Solution as the islet isolation solution, both before purification, and also during the purification in an innovative density gradient (3) — the foundation for current clinical purification methods. The talk was recorded on tape (except for the intro), and will be posted here soon too.

Notes

1) Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. Postprandial performance after canine islet transplantation: the importance of gut factors. 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991.

2) Guicherit OR, Van der Burg MPM, Lemkes HHPJ, Frölich M, Gooszen HG. Pancreatic
denervation causes reduced insulin sensitivity. 5 th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) Oct 7–10 1991.

3) Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 5th Congress European Society for Organ Transplantation, Maastricht (The Netherlands) October 7–10, 1991.

Citation info : Islet Transplant Performance 5th ESOT 1991 • Michel van der Burg • Miracles.Media • @1MEMO 20250109 • URL michelvanderburg.com/2025/01/09/

Greek Poster • @1MEMO 20241009

Greek Poster • Michel van der Burg • Miracles•Media • 20241009

Funny. LibreOffice Vanilla Issue.

Greek characters appeared in this poster originally designed with english text and produced on a Macintosh computer and Imagewriter dotmatrix printer, September 3, 1990, using the MacDraw II application, with graphs from Cricket Graph.
That 1990 Mac file was remastered today in LibreOffice Vanilla (other minor issues in graphs not corrected). Generally LibreOffice does a great job with these ancient files. I recently posted several of such posters at site michelvanderburg.com.
However, some of the posters retrieved from my archive show up with Greek characters when opened in LibreOffice, like this poster produced in English (with roman characters).
Those problematic posters have in common that the Apple Chicago font was used – the interface font in Apple’s early versions of Mac OS from 1984 to 1997. Clearly LibreOffice cannot handle this Chicago font correctly. I have not found a solution for this.
Text in other posters, produced with Helvetica, had only minor issues in LibreOffice, Helvetica italics text appeared as regular Times.

The original poster and abstract (below) were presented at the 8th International Symposium on Gastrointestinal Hormones, in Timmendorfer Strand Baltic Sea (FRG) September 4–8, 1990.

Abstract

Contribution of gut factors to canine isolated islet function
M.P.M. van der Burg, O.R. Guicherit, J.B.M.J. Jansen, C.B.H.W. Lamers, J.P. Scherft, M. Frölich, H.G. Gooszen.
Departments of Surgery, Gastroenterology. Endocrinology and Cel Biology. University Hospital, Leiden, The Netherlands.

Techniques for isolating islets of Langerhans from the large mammalian pancreas have improved to the point where islet transplantation as a therapeutical approach to human diabetes has become more realistic. The quality of metabolic control is however largely unknown. We studied canine isolated-islet function both in vivo after autotransplantation, as well as in vitro by perifusion.
Five normal dogs underwent total pancreatectomy. Islets were isolated from the excised pancreas by collagenase digestion, dispersion and purification with filtration and density gradients. Isolated islets were autotransplanted into the spleen of the dog by retrograde venous infusion. Graft function was assessed up to 3 mo by determining the glucose and insulin response to an intravenous glucose injection (IVGTT), i.v. arginine injection during 35 mM glucose clamp (AT), and a meal. In addition (n = 4) the in vitro insulin response of overnight cultured canine islets was studied by perifusion with cholecystokinin (CCK-33) and glucose-dependent insulinotropic polypeptide (GIP).
The islet dose at transplantation ranged from 3500 – 13000 islets/kg b.w. One animal became overtly hyperglycemic (fasting glucose 18 mM) within 7 days after receiving 3500 islets/kg b.w. The other grafts (> 6000 islets/kg b.w.) were successful (fasting glucose <7mM) but demonstrated, compared to pre-operative values, a 50% reduced glucose clearance and insulin response at IVGTT, and a 90% reduced insulin secreting capacity at AT. Postprandially hyperglycemia (~ 10 mM) and in contrast to i.v. glucose and arginine, a normal insulin response was observed. The in vitro insulin response at 7.5 mM glucose to equimolar (0.1-1- 10 nM) concentrations of either CCK, GIP or both, demonstrated a sustained dose-response to GIP (up to 5x basal values) and a significant albeit small and transient effect of CCK as of 1 nM. No synergistic effect was observed.
In dogs ‘one-to-one’ transplantation was successful in 4/5 recipients of isolated islets. The difference in the effect of islet transplantation on the insulin response to intravenous glucose or – arginine and a meal, may be related to the postprandial, hyperglycemia enhanced, activation of the entero-insular axis, especially GIP.

Van der Burg MPM, Guicherit OR, Jansen JBMJ, Lamers CBHW, Scherft JP, Frölich M, Gooszen HG. Contribution of gut factors to canine isolated islet function (Abstract). Digestion 1990; 46 (Suppl 1): 14–15. https://doi.org/10.1159/000200356

PDF abstract shown below – file 20241009_1_Digestion.pdf

Citation info : Greek Poster • Michel van der Burg • Miracles•Media • 20241009 • URL michelvanderburg.com/2024/10/09 | TakeNode 10afaddd-08b7-4dcd-ad7a-43228420ad59

Gut factors controlling pancreatic islets • 20240910

Function after intrasplenic transplantation of islets of Langerhans in dogs.

The effect of gut hormones GLP-1 , GIP , and CCK-33 together with glucose on the insulin secretion capacity of isolated islets tested during storage of the islets in the laboratory (in vitro by perifusion), and after transplantation (in vivo).

Dutch poster (1) presented at the Surgeon Days ’92 of the Dutch Surgery Society, Rotterdam (The Netherlands) May 21–22, 1992.

An English edition of this presentation was presented in 1991, without the GLP-1 data (2) and posted again Sep 6, 2024 (3).

The poster was originally designed and produced on a Macintosh computer and Imagewriter dotmatrix printer, May 1992, using the MacDraw II application, with graphs from Cricket Graph. That 1992 Mac file was remastered today in LibreOffice Vanilla.

Notes

1. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Terpstra JL, Gooszen HG. Functie na intralienale transplantatie van eilandjes van Langerhans in de hond ( Function after intrasplenic transplantation of islets of Langerhans in dogs ). Surgeon days ’92 of the Dutch Surgery Society, Rotterdam (The Netherlands) May 21–22, 1992.

2. Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. CCK and GIP control of canine isolated islet function. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

3. Gut feelings of pancreatic islets • 20240906 | Michel van der Burg | michelvanderburg•com | URL https://michelvanderburg.com/2024/09/06/gut-feelings-of-pancreatic-islets-20240906/

4. Van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide, and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Peptides 1995; 60: 61–67.

5. Pancreatic islet transplantation Thesis Repository Leiden University | 20221125 | Michel van der Burg | Miracles•Media | URL https://michelvanderburg.com/2022/11/25/pancreatic-islet-transplantation-thesis-repository-leiden-university-20221125/

Citation info : Gut factors controlling pancreatic islets • 20240910 | Michel van der Burg | michelvanderburg•com | TakeNode 56ddc057-ffe9-4b70-bd33-180ea4b07877

Gut feelings of pancreatic islets • 20240906

The effect of gut hormones together with glucose on the insulin secretion capacity of isolated islets tested during storage of the islets in the laboratory, and after transplantation.

CCK and GIP control of canine isolated islet function.

Poster (1) presented at the 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

The poster was originally designed and produced on a Macintosh computer and Imagewriter dotmatrix printer, June 3, 1991, using the MacDraw II application, with graphs from Cricket Graph. That 1991 Mac file was remastered today in LibreOffice Vanilla.

Notes

1. Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. CCK and GIP control of canine isolated islet function. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

2. Pancreatic islet transplantation Thesis Repository Leiden University | 20221125 | Michel van der Burg | Miracles•Media | URL https://michelvanderburg.com/2022/11/25/pancreatic-islet-transplantation-thesis-repository-leiden-university-20221125/

Citation info : Gut feelings of pancreatic islets • 20240906 | Michel van der Burg | michelvanderburg•com | TakeNode 5e52cddc-469d-4f91-bd23-e833566be41e

Updates • 20240808

Lyon 1991 Percoll Poster | 20240731 • 20240808 | Michel van der Burg | michelvanderburg•com | TakeNode 8cd3161a-2405-4586-aaeb-08096e1a636e

Two posts have been updated / upgraded :

  1. Leiden Islet Laboratory History ( 20240803 ) was updated last night , by adding some history notes. | URL https://michelvanderburg.com/2024/08/03/leiden-islet-laboratory-history-20240803/
  2. Percoll UWS Pancreatic Islet Purification (20240731) now has a remastered poster image , see below.

Lyon 1991 Percoll Poster | 20240731 • 20240808

20240808 – remastered poster : Following the first publication here last month (20240731) of the original 1991 MacDraw poster opened (converted) in LibreOffice Vanilla (1), I learned how to do some editing in the LibreOffice app to further restore poster layout details such as line spacing, and font style. An archive photo of another poster presentation in Pittsburgh in 1992 (that will be posted here soon) guided this restoration.

Current workflow :
1) opening the MacDraw II file in LibreOffice Vanilla
2) editing in in LibreOffice Vanilla , saving as LibreOffice Vanilla file (OpenDocument Drawing – extension odg).
3) opening the photo tiff image in the Mac’s Preview app, and copying the displayed image to the clipboard
4) pasting the clipboard image in the LibreOffice doc , and dragging to adjust size and location 5) exporting the file as PDF (image resolution 300 DPI ; PDF/A-1b ; PDF/UA). 6) Converting the PDF in the Preview app to TIFF 144 pixels/inch. 7) Adding caption , metadata etc with JPEG conversion in PhotoMill.

The 20240731 post now has this updated 20240808 – poster image.

Citation info : Lyon 1991 Percoll Poster | 20240731 • 20240808 | Michel van der Burg | michelvanderburg•com | TakeNode 8cd3161a-2405-4586-aaeb-08096e1a636e

PentaStarch UWS Pancreatic Islet Purification • 20240802

Following first introduction of the use UWS (the UW organ preservation solution) for pancreatic islet isolation in 1989 , next also purification of the islets in UWS was presented in 1991, with two options.
One option was an iso-osmotic density gradient solution of Percoll in UWS. The second option was a density gradient solution created by increasing the Pentastarch concentration in UWS (7) . Note that Pentastarch is a component of UWS.

The Percoll-UWS poster was posted recently (8)
Here the remastered poster presentation on Pentastarch-UWS :
Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

The poster was designed and produced on my personal Macintosh computer and Imagewriter dotmatrix printer, the night of June 3-4, 1991, using the MacDraw II application.
The Apple Imagewriter-II matrix printer, allows printing landscape across a chain of several sheets of paper, strips, the width of the poster (here 3 sheets long). The strips of the poster (here 3 strips) were then glued on site along the perforation, creating the 36/24 inch poster.
Finally the photo print of the purified islets was pasted in the poster at the spot at the venue in Lyon. In Lyon a photo of a dithizone stained Pentastarch purified islet sample was shown. To save time the archive was not searched for that image, and another image of Pentastarch purified islets was inserted in this remastered edition, a reflection contrast micrograph of an ultrathin section of Pentastarch purified islets immuno-stained with gold on insulin in the islet cells.

Nowadays, it is a challenge opening such old Mac files … Luckily, I was able to open this MacDraw II poster file in LibreOffice Vanilla and now learned to use the app to further correct most conversion issues. Pixelmator Pro was used for additional ‘repair’ and insertion of the photo scan.

Notes

1. Minneapolis 1989 Poster UW Solution Islet Isolation | 20220822 | Michel van der Burg | michelvanderburg•com | URL https://michelvanderburg.com/2022/08/22/minneapolis-1989-poster-uw-solution-islet-isolation-20220822/

2. Van der Burg MPM, Guicherit OR, Prins FA, Scherft JP, Frölich M, Gooszen HG. Percoll in UW: efficient iso-osmotic canine and human islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

3. Van der Burg MPM, Guicherit OR, Ploeg RJ, Scherft JP, Frölich M, Gooszen HG. Pentastarch in UW (SUW): a new method for complete islet purification. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

4. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Gooszen HG. Sigma’s industrial grade dextran is not suitable for islet isolation. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

5. Van der Burg MPM, Guicherit OR, Ebert R, Jansen JBMJ, Creutzfeld W, Lamers CBHW, Frölich M, Gooszen HG. CCK and GIP control of canine isolated islet function. 3rd International Congress on Pancreatic and Islet Transplantation, Symposium on Artificial Insulin Delivery System, Lyon (France) June 6–8, 1991.

6. Pancreatic islet transplantation Thesis Repository Leiden University | 20221125 | Michel van der Burg | Miracles•Media | URL https://michelvanderburg.com/2022/11/25/pancreatic-islet-transplantation-thesis-repository-leiden-university-20221125/

7. Pentastarch is the synthetic colloid component of UWS (ViaSpan / Belzer solution), a purified fraction (250 kD) of hydroxyethyl starch. Pentastarch powder was used here to increase the Pentastarch concentration and density of the UWS.

8. Percoll UWS Pancreatic Islet Purification • 20240731 | Michel van der Burg | michelvanderburg•com

Citation info : PentaStarch UWS Pancreatic Islet Purification • 20240802 | Michel van der Burg | michelvanderburg•com | TakeNode d11f1cef-a343-4466-8814-3e52a0261cae