Human Islet Purification Miami 1998 • 20181102

Efficacy of the novel iodixanol-UWS density gradient for human islet purification

Slideshow (video) of my January 1999 oral presentation in Igls (Innsbruck), Austria, at the 18th Workshop of the AIDSPIT (Artificial Insulin Delivery Systems, Pancreas and Islet Transplantation) Study Group. (Now known as AIDPIT).

These results of this innovative islet purification method developed in our Leiden islet laboratory (LUMC) were the outcome of work I had done spring 1998 with the islet group of Camillo Ricordi in the “fast track” center for diabetes research at the Diabetes Research Institute in Miami , Florida, USA.

Notes

The slideshow (pdf) was first uploaded Nov. 11, 2010 at at Scribd , and Archive.org • URL http://archive.org/details/EfficacyOfTheNovelIodixanol-uwsDensityGradientForHumanIslet

Original video title (file name) : 20181102 ~ Human Islet Purification Miami 1998

Updates:
20241202 – Text updated with extra info. Youtube video in post replaced by Vimeo edition. Copied 1-memo post to michelvanderburg.com .

Citation info : Human Islet Purification Miami 1998 • Michel van der Burg • Miracles.Media • 20181102 • URL michelvanderburg.com/2018/11/02/ • TakeNode 3f2ec4d2-afe0-4580-b055-92a2f231a4fa

Lessons From Clinical Islet Transplantation

① memo 20170208 ~ Lessons From Clinical Islet Transplantation ~ Chairman K. Federlin (Giessen, Germany) opened the seminar Tuesday, January 25, 1994 and David Scharp started with his lessons from the Center in St. Louis (USA) – 13th Workshop of the AIDSPIT Study Group , Igls, Austria ( note : sound re-recorded from speaker of Olympus mini-tape dictaphone )

24th AIDPIT Workshop Igls • @1MEMO 20160125

24th Workshop of the AIDPIT Study Group (Artificial Insulin Delivery, Pancreas and Islet Transplantation), in Igls (Innsbruck) Austria, 23-25 January 2005. Short impression filmed January 25th, further details in endcard of film.

#AIDPIT #Igls #Innsbruck #Tyrol #history #AIDSPIT #Austria #islet #Langerhans #isolation #transplantation #pancreas #diabetes #congress #insulin #michelvanderburg #symposium #EASD

Update

Update 20260125 : The original video embedded (Vimeo file, 640×480) is replaced by embedding a second, remastered, video edition (2022) produced by upscaling the original video file (converted to size 1920 × 1440), adding a caption, and uploaded to YouTube Jan 25, 2022. Post text changed conform current layout. A more detailed post is published in addition, URL https://1-memo.com/2026/01/25/

Citation info : 24th AIDPIT Workshop – Igls • Michel van der Burg • Miracles•Media • @1MEMO 20160125 • TakeNode 3baced38-ee79-4127-932d-0136293529e7 • URL https://1-memo.com/2016/01/25/

Out Of Service | 20140911

Out Of Service | 20140911 | 1-memo•com | In the morning of 9/11 2001 logistics forced me to officially announce a temporarily – 1 week – ‘out of service’ of our Leiden islet isolation and transplant center. Our center was started earlier that summer of 2001 at the Leiden University Medical Center. So, I decided to have a short vacation abroad – heaven, after being on call 24/7 almost non stop for years. Later that day at home, I was downstairs watching TV — because upstairs near my workroom the central heating was being replaced – when I saw the CNN breaking news about the plane crashing into the World Trade Center. That day would become a landmark in history and the course of my life.

Background image : Microscopy picture I took of purified human Islets of Langerhans – tiny clumps (size around 1/10 mm or 1/25 inch) of 10s to 1000s of cells that produce hormones like insulin – these cells are just visible when you enlarge this 50x microscopy picture : as tiny translucent ‘balls’ or ‘spheres’. The red color is from a stain that binds to the insulin cells.
Iodixanol-UWS purified human islets, stained with dithizone. Original magnification 50x.

Title: mvdb20041118_114555m0400.tif
Creator: Michael P.M. van der Burg
Caption/Description: Iodixanol-UWS purified human islets, stained with dithizone. Original magnification 50x. Copyright Notice: Copyright © 2004 M.P.M. van der Burg. All rights reserved. Digital Creation Date: 18 Nov 2004

References

Image published in : Transplantatie van eilandjes van Langerhans bij patiënten met diabetes mellitus type 1. Ned Tijdschr Geneeskd 2005 1 januari;149(1) – URL https://www.ntvg.nl/system/files/publications/2005100120001a.pdf

Efficacy of the novel iodixanol-UWS density gradient for human islet purification (Presentation publ. Archive Org – michelvanderburg, 19990125 )
https://archive.org/details/EfficacyOfTheNovelIodixanol-uwsDensityGradientForHumanIslet

25 Years of the Ricordi Automated Method for Islet Isolation. Piemonti L, Pileggi A. in CellR4 2013; 1(1): e128. – URL https://www.cellr4.org/article/128

Credit : Out Of Service | 20140911 | 1-memo•com | TakeNode ID e22f209c-14da-40d8-b11a-74a29b1e80b4

TAGS #michelvanderburg #1Memo #oncall #LeidenUniversity #OutOfService #Islet #Langerhans #isolation #purification #transplantation #clinic #human #center #diabetes #CNN #WorldTradeCenter #breakingnews #crash #CV #LUMC #microscopy #dithizone #stain #cell #insulin #therapy #Iodixanol #UWS #UniversityofWisconsin #solution

25 Year Islet Transplants

July 3, 2014 – Holland, Leiden University Hospital, 25 years ago , short excerpt – highlighting the moment of transplanting – from the 19890613 surgery journal at our start of the first pre-clinical succesfull islets graft transplants in the Netherlands and most of Europe, using a simple yet very novel approach that eventually recently became the worldwide -usa at least- standard for transplanting clinical grade purified islets in insulin-dependent diabetic patients – link to article on the 25 year timeline of progress in research and patient treatment , here : http://www.cellr4.org/article/128 .

25 Year Islet Transplants | 20140703 | 1-memo.com