Glycemic control mechanisms after canine islet autografting • IPITA 1995• @1MEMO 20251204

Glycemic control mechanisms after canine islet autografting • IPITA 1995 • Michel van der Burg • Miracles.Media • @1MEMO 20251204_1 • Talk, Tuesday June 20, 1995 at 10:30, 5th IPITA, Eden Roc Resort Hotel, Miami Beach, FL (USA) • Digitally remastered by author, publisher, Michel van der Burg • TakeNode 5227c200-e029-4406-b344-15350fe3b93d • #GLP1 #GIP #diabetes #islets #transplant

Pioneer work on GLP-1 and GIP incretin hormones

Slideshow (digitally remastered) of my talk Tuesday June 20, 1995 at 10:30, at the 5th International Congress on Pancreas and Islet Transplantation (IPITA), in Miami Beach, FL (USA). My first talk at a world congress presenting the outcome of all our detailed metabolic studies in our preclinical model of canine islet autotransplantation , including our pioneer work on the incretin effects of the gut hormones GLP-1 (glucagon-like peptide-1) and GIP (Glucose-dependent Insulinotropic Polypeptide) — now, in 2025, well-known from the drugs that mimic the action of these natural hormones, to treat type 2 diabetes and obesity, by stimulating insulin release, improving blood sugar control, and reducing appetite.

May 1992 we were the first to show direct potentiation of the insulin response from isolated islets by (near-)physiological doses of both the gut hormones GLP-1 and GIP during perifusion (in vitro) of canine islets at 7.5-10 mM glucose levels (1,2), and January 1994 we next confirmed the incretin effect of a (near-)physiological dose of GLP-1 after canine islet transplantation , in a poster presented at the 13th Workshop of the AIDSPIT (aka AIDPIT) Study Group , Igls, Austria, January 23–25, 1994 (3).

Background of the work was posted earlier this year (4) and the work was published Nov 1994 in my PhD thesis Pancreatic islet transplantation , available online (5).

Welcome Reception 5th IPITA on Sunday 18 June 1995, at the congress venue , the Eden Roc Resort Hotel, in Miami Beach, FL (USA) • Michel van der Burg • Miracles.Media • @1MEMO 20251204_2 • TakeNode 8eb04df1-8280-4de3-a206-4b63413c3025

June 1995, 5th IPITA Talk

Tuesday June 20, 1995 at 10:30, at the 5th International Congress on Pancreas and Islet Transplantation (IPITA), in Miami Beach, FL (USA), was my first talk at a world congress presenting the outcome of all our detailed metabolic studies in the canine islet transplants , including the incretin effect of GLP-1, and also our findings with isolated canine islets during perifusion, in vitro, of the direct insulinotropic effects of the gut hormones GIP and GLP-1 (6) . That slideshow presentation (digitally remastered*) is posted here (1MEMO 20251204_1).

The proceedings paper delivered at the 5th IPITA is included in the Notes (7) , as well as the referenced previous papers (8,9)

Notes

1. Van der Burg MPM, Guicherit OR, Scherft JP, Frölich M, Terpstra JL, Gooszen HG. Functie na intralienale transplantatie van eilandjes van Langerhans in de hond ( Function after intrasplenic transplantation of islets of Langerhans in dogs ). Surgeon days ’92 of the Dutch Surgery Society, Rotterdam (The Netherlands) May 21–22, 1992. In : Gut factors controlling pancreatic islets • 20240910 • Michel van der Burg • michelvanderburg•com • URL https://michelvanderburg.com/2024/09/10/gut-factors-controlling-pancreatic-islets-20240910/

2. Van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Pept. 1995 Dec 7;60(1):61-7. doi: 10.1016/0167-0115(95)00122-4. PMID: 8747785.

3. Metabolic control after canine islet transplantation. M.P.M. van der Burg, P.T.R. van Suylichem, O.R. Guicherit, J.H.M. van Deijnen, M. Frölich, and H.G. Gooszen (Universities of Leiden & Groningen, The Netherlands). Poster presented at the 13th Workshop of the AIDSPIT Study Group , Igls, Austria, January 23–25, 1994 • In : GLP-1 blood sugar regulation in islet transplants • AIDSPIT 1994 • Michel van der Burg • Miracles.Media • @1MEMO 20251114 • TakeNode 810cc881-d084-4d2e-b252-d432918b1946 • URL https://michelvanderburg.com/2025/11/14

4. Background of this work was posted in Dutch Islets Transplant Team • @1MEMO 20250125 • URL https://michelvanderburg.com/2025/01/25/dutch-islets-transplant-team-1memo-20250125/

5. Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation. (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Dec 4th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 .

6. Oral presentation : Van der Burg MPM, Van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glycemic control mechanisms after canine islet autografting. 5th International Congress on Pancreas and Islet Transplantation (IPITA), Miami Beach (USA) June 20, 1995.

7. 5th IPITA Proceedings publication : Van der Burg MPM, Van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glycemic control mechanisms after canine islet autografting. Transplant Proc. 1995 Dec;27(6):3187-8. PMID: 8539903. PDF available below PMID_8539903

PMID_8539903 Download

Referenced previous publications :

8. Van der Burg MPM, Gooszen HG. The metabolic efficiency of islet grafts: an overview. In: Islet transplantation — current status of clinical application and experimental results. Hesse UJ , Pichlmaier H, Eds., Lengerich Germany, Wolfgang Pabst Verlag, 1992, p.93–99. Pabst hash code 0383b3abad3ae774b146ea0425c2227a PDF available below 1MEMO_20251204_4

1MEMO_20251204_4 Download

9. Van der Burg MPM, Guicherit OR, Scherft JP, Lemkes HHPJ, Frölich M, Gooszen HG. Postprandial performance after canine islet transplantation: the importance of gut factors (Abstract). Neth J Med 1992; 41: A25. PDF available below 1MEMO_20251204_3

1MEMO_20251204_3 Download

Other previous publications :

10. Van der Burg MPM, Guicherit OR, Van Suylichem PTR, Frölich M, Van Deijnen JHM, Gooszen HG. Importance of the entero-insular axis for islet graft function (Abstract). Cell Transplant 1994; 3: 257. Abstract – Second International Congress of the Cell Transplant Society May 1–4, 1994 Hyatt Regency Hotel • Minneapolis, Minnesota . https://doi.org/10.1177/096368979400300302 (Abstracts published , posters not presented). PDF available below 10.1177_096368979400300302

10.1177_096368979400300302 Download

11. Chapter 8, Doctoral Thesis, Leiden University, November 1994 : Metabolic control after intrasplenic islet autotransplantation in dogs: ß-cell secretory capacity, insulin action, and the enteroinsular axis. Michael P.M. van der Burg, Paul T.R. van Suylichem, Onna R. Guicherit, Marijke Frölich, Herman H.P.J. Lemkes, and Hein G. Gooszen. In : Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation ( Chapter 8). (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Nov 24th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 .
Direct link to file Chapter 8 , URL https://scholarlypublications.universiteitleiden.nl/access/item%3A3486615/view .

12. Doctoral Thesis, Leiden University, November 1994 (Chapter 6) : lnsulinotropic properties of cholecystokinin, gastric inhibitory polypeptide, and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Michael P.M. van der Burg, Onna R. Guicherit, Marijke Frölich and Hein G. Gooszen. In : Van der Burg MPM. Pancreatic islet transplantation: studies on the technique and efficacy of islet isolation and transplantation ( Chapter 6). (Doctoral Thesis, Leiden University, 1994), Paperback (1994, Nov 9) Boskoop: Van der Burg (ISBN 9080216410); E-book (2022, Oct 25) Boskoop: Michel van der Burg | Miracles•Media ( ISBN 9789080216402). Retrieved (Nov 24th, 2025) from Leiden University Scholarly Publications, Persistent URL https://hdl.handle.net/1887/3486604 .
Direct link to file Chapter 6 , URL https://scholarlypublications.universiteitleiden.nl/access/item%3A3486613/view

Full papers

13. Van der Burg MPM, Guicherit OR, Frölich M, Gooszen HG. Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets. Regul Pept. 1995 Dec 7;60(1):61-7. doi: 10.1016/0167-0115(95)00122-4. PMID: 8747785.

14. Van der Burg MPM, Van Suylichem PTR, Guicherit OR, Frölich M, Lemkes HHPJ, Gooszen HG. Glucoregulation after canine islet transplantation: contribution of insulin secretory capacity, insulin action, and the entero-insular axis. Cell Transplant. 1997 Sep-Oct;6(5):497-503. doi: 10.1177/096368979700600509. PMID: 9331501. PDF link https://pure.rug.nl/ws/portalfiles/portal/58359942/096368979700600509.pdf

* 1MEMO 20251204_1 . Digitally remastered presentation, using the images from the original slides handout, and the handout of the text spoken during the original presentation , now read using my personal synthesized voice.

#GLP1 #GIP #diabetes #islets #transplant

Citation info : Glycemic control mechanisms after canine islet autografting • IPITA 1995 • Michel van der Burg • Miracles.Media • @1MEMO 20251204 • URL https://michelvanderburg.com/2025/12/04

Coding 1995 Internet Access Macintosh • @1MEMO 20250515

Screenshot April 11, 1995 at 21:48 of the desktop with a Filemaker window (•Brieffile, correspondence) on my Apple Macintosh PowerBook 160. A PICT file image that I recently gained access to.
Desktop shows icon (renamed) SLIP of the InterSLIP application I used to get modem access (phone line) to the Leiden University gateway to internet, email, news, gopher, ftp etc .
I wrote the protocol (two scripts) required by this SLIP application to get access to the Leiden University gateway to the internet.
From May 1995 Leiden University distributed my scripts to the other Macintosh users of our University.

Below the two scripts published May 18, 1995 ….

Below the ‘Minimal dialing script’ :

! Original script by Fred Morris
! Modifications and comments by Adam C. Engst
! 21-Feb-94 = Added redial feature (ACE)

@originate
! Initialize mode from string in InterSLIP configuration
note “Initializing modem”
write “^4\13”
pause 20
! Dial modem from number in InterSLIP configuration
note “Dialing ^1”
write “atdt^1\13”
! Check only for BUSY or CONNECT, then redial or connect
matchstr 1 10 “BUSY”
matchstr 2 20 “CONNECT”
matchread 500

@label 10
! The line is busy, so let’s redial the modem indefinitely
! This will also redial after the (matchread 500) timeout if you get
endless rings
note “Line busy, redialing ^1”
! First hang up and reinitialize modem from string in InterSLIP configuration
write “+++”
pause 60
write “ath\13”
write “^4\13”
! Then go back and dial again using phone number in InterSLIP configuration
jump originate

@label 20
! Everything worked fine, you’re connected now
note “Dialed successfully”
exit 0

@answer
@hangup
! Hang up the modem and reinitialize from string in InterSLIP configuration
! The only way to get here is to manually click the Disconnect button
note “Hanging up modem”
write “+++”
pause 60
write “ath\13”
write “^4\13”
exit 0

Below the “FullCRIgateScript” for access via 278282 with password and
username check:

! InterSLIP gateway script for CRIgate with password access
! original script by Adam C. Engst
! modified by MvdBurg@RULLF2.MedFac.LeidenUniv.NL (MPM van der Burg)
!
@originate
!write “\13”
note “Waiting for prompt”
matchclr
matchstr 1 1 “Annex username: “
matchread 900
note “Gateway not responding!”
exit -1
!
@label 1
note “Sending user name”
write “^5\13”
matchclr
matchstr 1 2 “Annex password:”
matchread 900
note “Gateway not responding!”
exit -1
!
!
@label 2
note “Sending password”
write “^6\13”
matchclr
matchstr 1 4 “Permission granted”
matchstr 2 3 “Username/Password Incorrect”
matchread 900
jump 99
!
@label 3
note “Username/Password Incorrect”
jump 99
!
@label 4
note “Requesting SLIP”
matchclr
matchstr 1 5 “drive-in: “
matchread 900
jump 99
!
@label 5
write “slip\13”
matchclr
matchstr 1 10 “Switching to SLIP.”
matchstr 2 6 “CLI: Command not found.”
matchread 900
note “Cannot invoke SLIP mode”
jump 99
!
@label 6
note “Command not found”
jump 99
!
@label 10
note “Searching IP address”
matchclr
matchstr 1 25 “Your address is “
matchread 900
note “No AddressLine found”
jump 99
!
@label 25
note “SLIP address found”
matchclr
matchexp 1 30 “[0-9][0-9]\.[0-9][0-9]\.[0-9][0-9]\.[0-9][0-9]\.”
matchread 500
note “No IP address found”
jump 99
!
@label 99
pause 1
pause 60
exit -1
!
! We’ve found an IP address, so inform MacTCP of it.
!
@label 30
note “Setting IP address”
setip “^0”
note “^0”
Pause 30
write “set port internet slip enable”
write “\13”
!
note “Slip enabled”
pause 20
!
matchclr
exit 0

!
! Answer and Hangup modes are currently unused in gateway
! scripts, but just for future compatibility, exit successfully.
!
@answer
@hangup
! Hang up the modem and reinitialize from string in InterSLIP configuration
! The only way to get here is to manually click the Disconnect button
note “Hanging up modem”
write “+++”
pause 60
write “ath\13”
write “^4\13”
exit 0

TAGs :
#coding #script #modem #dial #Leiden #University #SLIP #Apple #Powerbook #Mosaic #internet #history #desktop #intransition

Citation info : Coding 1995 Internet Access Macintosh • Michel van der Burg • Miracles•Media • @1MEMO 20250515 • TakeNode • URL 1-memo.com/2025/05/15 •

Daily Cookbook • @1MEMO 20250209

‘Spruitjessoep’ , dutch Brussels sprout soup, in the Daily Cookbook (Dagkookboek by Marianne Stuit) I bought new — two copies for both me and my friend — in 1971-2. I was living in Oegstgeest, Holland, near Leiden, as a student Biology (Biochemistry) at the Leiden University. I vividly remember, sliding across the floor (with a sprained ankle) between my tiny kitchen and student room – in the attic of my landlady’s house – with a large enamel pot of Brussels sprout soup.

Citation info : Daily Cookbook • Michel van der Burg • Miracles.Media • @1MEMO 20250209 • TakeNode 092c41bb-18f0-4430-adb4-74b66b1c6933

Fresh vs Cultured Pig Islets for Xenografts • NTV Bootcongres 1998 • @1MEMO 20250201

Fresh vs Cultured Pig Islets for Xenografts • NTV Bootcongres 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250201_1 • TakeNode 71d39e7e-ab5e-42b6-8cf2-a17d464ce132

Van der Burg MPM, Rijkelijkhuizen JKRA, Zwaan RP, Bouwman E. Culture of isolated pig islets pre-xenotransplantation greatly improves the quality and survival of the graft (Kweek van geïsoleerde varkenseilandjes pre-xenotransplantatie verbetert aanzienlijk de kwaliteit en overleving van het transplantaat). Tenth Congress of the Dutch Transplantation Society (Bootcongres 1998), Kerkrade (The Netherlands) April 21–23, 1998.

Download (pdf) NTV bulletin (abstract) & poster , file 1MEMO_20250201

1MEMO_20250201🔗Download

EN – Abstract

PRE-TRANSPLANT SHORT-TERM CULTURE OF ISOLATED PIG ISLETS MARKEDLY IMPROVES THE QUALITY AND SURVIVAL OF THE XENOGRAFT
Van der Burg MPM,* Rijkelijkhuizen JKRA, Zwaan RP, and Bouwman E, Leiden University Medical Centre, Leiden, The Netherlands.

Pre-transplant culture of isolated islets reduces the graft’s immunogenicity in various models. Little is known, however, on the effect of culture in pig islet xenotransplantation — probably, because porcine islets have been found difficult to culture. Because pilot transplants of freshly-isolated pig islets in strongly immunosuppressed rats resulted in primary non function, we first studied 1-7 day culture of the isolated pig islets at 37°C in RPMI plus 10% porcine serum. Islets were isolated from large sows (n =6) by an improved method, resulting in intact islets (size 185±16 µm) and no loss during purification, yielding 2448 islets (IEQs)/g with a >95% purity and 90±2% viability as assessed by acridine orange – propidium iodide (AOPI) staining. During culture, however, islet recovery was 24±9% at day 1 and 17±6% at day 7 (NS vs day 1). In order to delineate whether the culture conditions or the quality of freshly isolated islets caused the islet loss during culture, we compared graft survival in nude mice at 1 mo after transplantation under the kidney capsule of ~2500 fresh islets in normoglycemic recipients or ~1000 cultured islets in STZ-diabetic (>20 mM) recipients. After transplanting fresh islets, histology of the kidneys — sectioned every 500 µm — demonstrated substantial scarring and (near-)absence of islets. Cultured islet transplants, by contrast, rendered 5/6 recipients normoglycemic, and showed a substantial mass of well-preserved islets with little scarring at the grafts’ site. Thus, viability by AOPI-staining of fresh islets poorly predicts the survival in vivo and in vitro. A non-immune mediated disintegration of part of the fresh islets may substantially reduce the functional capacity of the graft, both direct by lowering the effective islet dose, and indirect first because scarring may hamper the engraftment of viable tissue, and second because the cellular debris most probably will attract macrophages and induce the release of harmful cytokines.

Citation info : Fresh vs Cultured Pig Islets for Xenografts • NTV Bootcongres 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250201 • URL michelvanderburg.com/2025/02/01/

Iodixanol-UWS Purification for Porcine Islet Transplantation • AIDSPIT 1998 • @1MEMO 20250131

Iodixanol-UWS Purification for Porcine Islet Transplantation • AIDSPIT 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250131

Slideshow (digitally remastered) of first talk on pancreatic islet xeno-transplantation following adult pig islet purification in our novel Iodixanol-UWS density gradient. Presented at the 17th Workshop of the AIDSPIT Study Group in Igls, Austria, Tuesday, Jan 27, 1998 (1, 2).

Isolation of porcine pancreatic islets (from the pancreas of large sows) was markedly improved by keeping the islets in the UW donor organ preservation solution (UWS) both during the isolation of islets (using the Liberase enzyme blend) and purification of the islets using a novel Iodixanol-UWS density gradient.
Transplantation of the pig islets (after 1 day storage in culture solution) restored normal blood sugar levels in diabetic mice.

Notes

1) Van der Burg MPM, Zwaan RP, Bouwman E. Markedly improved outcome of adult porcine islet isolation, purification, and culture using Liberase-PI versus collagenase-P, and a novel gradient of Optiprep in University of Wisconsin solution. 17th Workshop of the Study Group on Artificial Insulin Delivery Systems, Pancreas and Islet Transplantation (AIDSPIT) of the European Association for the Study of Diabetes (EASD), Igls (Austria) January 25—27,1998

2) Van der Burg MPM, Zwaan RP, Bouwman E. Markedly improved outcome of adult porcine islet isolation, purification, and culture using Liberase-PI versus collagenase-P, and a novel gradient of Optiprep in University of Wisconsin solution (Abstract). Horm Metab Res 1998; 30(1): A23 . DOI: 10.1055/s-2007-978834 .
Download abstract 1MEMO_20250131_2 below :

1MEMO_20250131_2🔗Download

Citation info : Iodixanol-UWS Purification for Porcine Islet Transplantation • AIDSPIT 1998 • Michel van der Burg • Miracles.Media • @1MEMO 20250131 • TakeNode 1f185515-7aeb-44ac-b721-2cec76df67b7 • URL michelvanderburg.com/2025/01/31/

Innovative Islet Purification in Iodixanol-UWS • IPITA 97 • @1MEMO 20250128

No porcine islet loss during density gradient purification in a novel iodixanol in University of Wisconsin solution. First talk on our introduction of the innovative Iodixanol-UWS density gradients for islet purification , showing no islet loss in the difficult model of pig islet isolation — presented at the 6th IPITA congress in Milan (Italy) , Thursday, 25th september 1997 (1).

Background

In 1995 the Leiden Islet Lab had moved into the Surgery Lab in the new LUMC building, and the islet team had grown, focussing on xenotransplantation. With more people isolating pig islets, more convenient islet isolation solutions were tried.

Both Percoll-UWS (2) and Histopaque-UWS (3) were being used from 1992 in first series of islet isolations from juvenile pig pancreases.

August 1995, I started designing the clinical promising islet purification solution of Iodixanal in UW-Solution (UWS). I mixed Optiprep – a 60 % solution of iodixanol in water – with a 2-times concentrated UWS, to prepare this density gradient solution. The first standard operation procedure of this Iodixanol-UWS gradient for our pig islet isolation research was completed November 1995.

This purification solution was a logical follow-up of our highly succesful Percoll-UWS density gradient (2) — a similar solution of impermeants (that do not readily enter cells) with similar osmolarity (close to that of the tissue) , more practical though, and with the important advantage that iodixanol is clinical tested.

July 1996, I introduced the Iodixanol-UWS gradient to replace the Percoll-UWS gradient for large scale pancreatic islet research in this difficult model of islet isolation from juvenile pig pancreases.

The work was presented September 1997. First a poster was presented that month at the Xenotransplantation Congress in Nantes , France (4). Next, two talks followed at the 6th Congress of the International Pancreas and Islet Transplant Association (IPITA) in Milan (Italy).

Here the (remastered) slideshow of that first talk at the 6th IPITA Congress in Milan, presented Thursday, 25th september 1997 (1) . The slideshow of the 2nd talk , presented at the last day of that congress, was posted yesterday (5).

Notes

1) Van der Burg MPM, Basir I, Bouwman E. No porcine islet loss during density gradient purification in a novel iodixanol in University of Wisconsin solution. Sixth Congress of the Int. Pancreas and Islet Transplant Association (IPITA), Milan (Italy) Sept. 24–27, 1997.

2) Percoll UWS Purification Pancreatic Islets • ESOT 1991 Talk • Michel van der Burg • Miracles.Media • @1MEMO 20250114 • URL michelvanderburg.com/2025/01/14/

3) Basir I, van der Burg MPM, Scheringa M, Töns A, Bouwman E. Improved outcome of pig islet isolation by Pefabloc inhibition of trypsin. Transplant Proc. 1997 Jun;29(4):1939-41. doi: 10.1016/s0041-1345(97)00168-1. PMID: 9193462

4) Van der Burg MPM, Basir I, Bouwman E. Non-traumatic porcine islet density gradient purification in a novel iodixanol in University of Wisconsin solution. The 4th Int. Congress for Xenotransplantation, Nantes (France) Sept. 7–11, 1997. Online in : Purification Islets in Iodixanol-UWS • Leiden Start 1996 • Michel van der Burg • Miracles.Media • @1MEMO 20250126 • URL michelvanderburg.com/2025/01/26/

5) Van der Burg MPM, Basir I, Zwaan RP, Bouwman E. Porcine islet preservation during isolation in University of Wisconsin solution. Sixth Congress of the Int. Pancreas and Islet Transplant Association (IPITA), Milan (Italy) Sept. 24–27, 1997. Online in : Porcine Islet Preservation In UWS • IPITA 97 • Michel van der Burg • Miracles.Media • @1MEMO 20250127• URL michelvanderburg.com/2025/01/27/

Milan Papers • @1MEMO 20250205 • Manuscripts (Preprints) and Abstracts copies of the presentations at the IPITA 1997 meeting in Milan (Italy). PDF downloads at michelvanderburg•com/2025/02/05/

Citation info : Innovative Islet Purification in Iodixanol-UWS • IPITA 97 • Michel van der Burg • Miracles.Media • @1MEMO 20250128 • TakeNode • URL michelvanderburg.com/2025/01/28/

Porcine Islet Preservation In UWS • IPITA 97 • @1MEMO 20250127

This purification solution was a logical follow-up of our highly succesful Percoll-UWS density gradient — a similar solution of impermeants (that do not readily enter cells) with similar osmolarity (close to that of the tissue) , more practical though, and with the important advantage that iodixanol is clinical tested.

The Leiden Islet Lab had just moved into the Surgery Lab in the new LUMC building, and the islet team had grown, focussing on xenotransplantation. With more people isolating pig islets, more convenient solutions were tried.

Both Percoll-UWS and Histopaque-UWS were being used from 1992 for small scale islet isolations from juvenile pig pancreases.

July 1996, I introduced the Optiprep-UWS gradient to replace our Percoll-UWS gradient for larger scale porcine islet isolations.

The work was first presented September 1997, both in a poster presented at the Xenotransplantation Congress in Nantes , France (1), and later that month in two talks at the 6th Congress of the International Pancreas and Islet Transplant Association (IPITA) in Milan (Italy). The slideshow of the first talk (2) will be online at this site soon.
Here the remastered slideshow of the 2nd talk at the 6th IPITA Congress in Milan, presented Saturday, 27th September, 1997 (3).

Notes

1) Van der Burg MPM, Basir I, Bouwman E. Non-traumatic porcine islet density gradient purification in a novel iodixanol in University of Wisconsin solution. The 4th Int. Congress for Xenotransplantation, Nantes (France) Sept. 7–11, 1997. Online in : Purification Islets in Iodixanol-UWS • Leiden Start 1996 • Michel van der Burg • Miracles.Media • @1MEMO 20250126 • URL michelvanderburg.com/2025/01/26/

2) Van der Burg MPM, Basir I, Bouwman E. No porcine islet loss during density gradient purification in a novel iodixanol in University of Wisconsin solution. Sixth Congress of the Int. Pancreas and Islet Transplant Association (IPITA), Milan (Italy) Sept. 24–27, 1997.

3) Van der Burg MPM, Basir I, Zwaan RP, Bouwman E. Porcine islet preservation during isolation in University of Wisconsin solution. Sixth Congress of the Int. Pancreas and Islet Transplant Association (IPITA), Milan (Italy) Sept. 24–27, 1997.

Update 20250128 – update intro design timeline

Update 20250205 – added Milan papers

Citation info : Porcine Islet Preservation In UWS • IPITA 97 • Michel van der Burg • Miracles.Media • @1MEMO 20250127 • TakeNode 6d6fca2e-014c-40cf-8110-725b267c0b0e • URL michelvanderburg.com/2025/01/27/